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Gene expression profile of mouse bone marrow stromal cells determined by cDNA microarray analysis

Wieczorek, Georg; Steinhoff, Christine; Schulz, Ralph; Scheller, Marina; Vingron, Martin; Ropers, H-Hilger and Nuber, Ulrike LU (2003) In Cell and Tissue Research1974-01-01+01:00 311(2). p.227-237
Abstract
Bone marrow stromal cells (BMSC) have gained increased attention because of their multipotency and adult stem cell character. They have been shown to differentiate into other cell types of the mesenchymal lineage and also into non-mesenchymal cells. The exact identity of the original cells, which are isolated from bone marrow by their selective adherence to plastic, remains unknown to date. We have established and characterized mouse BMSC cultures and analyzed three independent samples by cDNA microarrays. The expression profile was compared with two previous expression studies of human BMSC and revealed a high degree of concordance between different techniques and species. To gain clues about the positional context and biology of the... (More)
Bone marrow stromal cells (BMSC) have gained increased attention because of their multipotency and adult stem cell character. They have been shown to differentiate into other cell types of the mesenchymal lineage and also into non-mesenchymal cells. The exact identity of the original cells, which are isolated from bone marrow by their selective adherence to plastic, remains unknown to date. We have established and characterized mouse BMSC cultures and analyzed three independent samples by cDNA microarrays. The expression profile was compared with two previous expression studies of human BMSC and revealed a high degree of concordance between different techniques and species. To gain clues about the positional context and biology of the isolated cells within the bone marrow stroma, we searched our data for genes that encode proteins of the extracellular matrix, cell adhesion proteins, cytoskeletal proteins and cytokines/cytokine receptors. This analysis revealed a close association of BMSC with vascular cells and indicated that BMSC resemble pericytes. (Less)
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author
publishing date
type
Contribution to journal
publication status
published
subject
in
Cell and Tissue Research1974-01-01+01:00
volume
311
issue
2
pages
227 - 237
publisher
Springer
external identifiers
  • pmid:12596042
  • scopus:0037294008
ISSN
1432-0878
DOI
10.1007/s00441-002-0671-3
language
English
LU publication?
no
id
b21b06e9-9272-4cb7-87af-f9cf77eb796f (old id 1128146)
date added to LUP
2008-06-04 09:40:46
date last changed
2018-05-29 09:41:15
@article{b21b06e9-9272-4cb7-87af-f9cf77eb796f,
  abstract     = {Bone marrow stromal cells (BMSC) have gained increased attention because of their multipotency and adult stem cell character. They have been shown to differentiate into other cell types of the mesenchymal lineage and also into non-mesenchymal cells. The exact identity of the original cells, which are isolated from bone marrow by their selective adherence to plastic, remains unknown to date. We have established and characterized mouse BMSC cultures and analyzed three independent samples by cDNA microarrays. The expression profile was compared with two previous expression studies of human BMSC and revealed a high degree of concordance between different techniques and species. To gain clues about the positional context and biology of the isolated cells within the bone marrow stroma, we searched our data for genes that encode proteins of the extracellular matrix, cell adhesion proteins, cytoskeletal proteins and cytokines/cytokine receptors. This analysis revealed a close association of BMSC with vascular cells and indicated that BMSC resemble pericytes.},
  author       = {Wieczorek, Georg and Steinhoff, Christine and Schulz, Ralph and Scheller, Marina and Vingron, Martin and Ropers, H-Hilger and Nuber, Ulrike},
  issn         = {1432-0878},
  language     = {eng},
  number       = {2},
  pages        = {227--237},
  publisher    = {Springer},
  series       = {Cell and Tissue Research1974-01-01+01:00},
  title        = {Gene expression profile of mouse bone marrow stromal cells determined by cDNA microarray analysis},
  url          = {http://dx.doi.org/10.1007/s00441-002-0671-3},
  volume       = {311},
  year         = {2003},
}