Gene expression profile of mouse bone marrow stromal cells determined by cDNA microarray analysis
(2003) In Cell and Tissue Research 311(2). p.227-237- Abstract
- Bone marrow stromal cells (BMSC) have gained increased attention because of their multipotency and adult stem cell character. They have been shown to differentiate into other cell types of the mesenchymal lineage and also into non-mesenchymal cells. The exact identity of the original cells, which are isolated from bone marrow by their selective adherence to plastic, remains unknown to date. We have established and characterized mouse BMSC cultures and analyzed three independent samples by cDNA microarrays. The expression profile was compared with two previous expression studies of human BMSC and revealed a high degree of concordance between different techniques and species. To gain clues about the positional context and biology of the... (More)
- Bone marrow stromal cells (BMSC) have gained increased attention because of their multipotency and adult stem cell character. They have been shown to differentiate into other cell types of the mesenchymal lineage and also into non-mesenchymal cells. The exact identity of the original cells, which are isolated from bone marrow by their selective adherence to plastic, remains unknown to date. We have established and characterized mouse BMSC cultures and analyzed three independent samples by cDNA microarrays. The expression profile was compared with two previous expression studies of human BMSC and revealed a high degree of concordance between different techniques and species. To gain clues about the positional context and biology of the isolated cells within the bone marrow stroma, we searched our data for genes that encode proteins of the extracellular matrix, cell adhesion proteins, cytoskeletal proteins and cytokines/cytokine receptors. This analysis revealed a close association of BMSC with vascular cells and indicated that BMSC resemble pericytes. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1128146
- author
- Wieczorek, Georg ; Steinhoff, Christine ; Schulz, Ralph ; Scheller, Marina ; Vingron, Martin ; Ropers, H-Hilger and Nuber, Ulrike LU
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Cell and Tissue Research
- volume
- 311
- issue
- 2
- pages
- 227 - 237
- publisher
- Springer
- external identifiers
-
- pmid:12596042
- scopus:0037294008
- pmid:12596042
- ISSN
- 1432-0878
- DOI
- 10.1007/s00441-002-0671-3
- language
- English
- LU publication?
- no
- id
- b21b06e9-9272-4cb7-87af-f9cf77eb796f (old id 1128146)
- date added to LUP
- 2016-04-01 11:34:04
- date last changed
- 2022-01-26 07:09:14
@article{b21b06e9-9272-4cb7-87af-f9cf77eb796f, abstract = {{Bone marrow stromal cells (BMSC) have gained increased attention because of their multipotency and adult stem cell character. They have been shown to differentiate into other cell types of the mesenchymal lineage and also into non-mesenchymal cells. The exact identity of the original cells, which are isolated from bone marrow by their selective adherence to plastic, remains unknown to date. We have established and characterized mouse BMSC cultures and analyzed three independent samples by cDNA microarrays. The expression profile was compared with two previous expression studies of human BMSC and revealed a high degree of concordance between different techniques and species. To gain clues about the positional context and biology of the isolated cells within the bone marrow stroma, we searched our data for genes that encode proteins of the extracellular matrix, cell adhesion proteins, cytoskeletal proteins and cytokines/cytokine receptors. This analysis revealed a close association of BMSC with vascular cells and indicated that BMSC resemble pericytes.}}, author = {{Wieczorek, Georg and Steinhoff, Christine and Schulz, Ralph and Scheller, Marina and Vingron, Martin and Ropers, H-Hilger and Nuber, Ulrike}}, issn = {{1432-0878}}, language = {{eng}}, number = {{2}}, pages = {{227--237}}, publisher = {{Springer}}, series = {{Cell and Tissue Research}}, title = {{Gene expression profile of mouse bone marrow stromal cells determined by cDNA microarray analysis}}, url = {{http://dx.doi.org/10.1007/s00441-002-0671-3}}, doi = {{10.1007/s00441-002-0671-3}}, volume = {{311}}, year = {{2003}}, }