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Mesenchymal stem cell content of human vertebral bone marrow

Ahrens, Norbert; Tormin, Ariane; Paulus, Margit; Roosterman, Daniela; Salama, Abdulgabar; Krenn, Veit; Neumann, Ulf and Scheding, Stefan LU (2004) In Transplantation 78(6). p.925-929
Abstract
Mesenchymal stem cells (MSCs) are capable of down-regulating alloimmune responses and promoting the engraftment of hematopoietic stem cells. MSCs may therefore be suitable for improving donor-specific tolerance induction in solid-organ transplantation. Cells from cadaveric vertebral bone marrow (V-BM), aspirated iliac crest-BM, and peripheral blood progenitor cells were compared. Cells were characterized by flow cytometry and colony assays. MSCs generated from V-BM were assayed for differentiation capacity and immunomodulatory function. A median 5.7 x 10(8) nucleated cells (NCs) were recovered per vertebral body. The mesenchymal progenitor, colony-forming unit-fibroblast, frequency in V-BM (11.6/10(5) NC, range: 6.0-20.0) was considerably... (More)
Mesenchymal stem cells (MSCs) are capable of down-regulating alloimmune responses and promoting the engraftment of hematopoietic stem cells. MSCs may therefore be suitable for improving donor-specific tolerance induction in solid-organ transplantation. Cells from cadaveric vertebral bone marrow (V-BM), aspirated iliac crest-BM, and peripheral blood progenitor cells were compared. Cells were characterized by flow cytometry and colony assays. MSCs generated from V-BM were assayed for differentiation capacity and immunomodulatory function. A median 5.7 x 10(8) nucleated cells (NCs) were recovered per vertebral body. The mesenchymal progenitor, colony-forming unit-fibroblast, frequency in V-BM (11.6/10(5) NC, range: 6.0-20.0) was considerably higher than in iliac crest-BM (1.4/10(5) NC, range: 0.4-2.6) and peripheral blood progenitor cells (not detectable). MSC generated from V-BM had the typical MSC phenotype (CD105(pos)CD73(pos)CD45(neg)CD34(neg)), displayed multilineage differentiation potential, and suppressed alloreactivity in mixed lymphocyte reactions. V-BM may be an excellent source for MSC cotransplantation approaches. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Transplantation
volume
78
issue
6
pages
925 - 929
publisher
Lippincott Williams & Wilkins
external identifiers
  • pmid:15385815
  • scopus:4644276628
ISSN
1534-6080
language
English
LU publication?
yes
id
8ed14f5c-44d1-4b54-8e0c-7c752a885c84 (old id 1129096)
date added to LUP
2008-06-12 11:27:45
date last changed
2017-09-10 04:27:11
@article{8ed14f5c-44d1-4b54-8e0c-7c752a885c84,
  abstract     = {Mesenchymal stem cells (MSCs) are capable of down-regulating alloimmune responses and promoting the engraftment of hematopoietic stem cells. MSCs may therefore be suitable for improving donor-specific tolerance induction in solid-organ transplantation. Cells from cadaveric vertebral bone marrow (V-BM), aspirated iliac crest-BM, and peripheral blood progenitor cells were compared. Cells were characterized by flow cytometry and colony assays. MSCs generated from V-BM were assayed for differentiation capacity and immunomodulatory function. A median 5.7 x 10(8) nucleated cells (NCs) were recovered per vertebral body. The mesenchymal progenitor, colony-forming unit-fibroblast, frequency in V-BM (11.6/10(5) NC, range: 6.0-20.0) was considerably higher than in iliac crest-BM (1.4/10(5) NC, range: 0.4-2.6) and peripheral blood progenitor cells (not detectable). MSC generated from V-BM had the typical MSC phenotype (CD105(pos)CD73(pos)CD45(neg)CD34(neg)), displayed multilineage differentiation potential, and suppressed alloreactivity in mixed lymphocyte reactions. V-BM may be an excellent source for MSC cotransplantation approaches.},
  author       = {Ahrens, Norbert and Tormin, Ariane and Paulus, Margit and Roosterman, Daniela and Salama, Abdulgabar and Krenn, Veit and Neumann, Ulf and Scheding, Stefan},
  issn         = {1534-6080},
  language     = {eng},
  number       = {6},
  pages        = {925--929},
  publisher    = {Lippincott Williams & Wilkins},
  series       = {Transplantation},
  title        = {Mesenchymal stem cell content of human vertebral bone marrow},
  volume       = {78},
  year         = {2004},
}