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Immunohistochemical loss of the DNA mismatch repair proteins MSH2 and MSH6 in malignant fibrous histocytomas

Ericson Lindquist, Kajsa LU ; Engellau, Jacob LU ; Persson, Annette LU ; Lindblom, Annika; Domanski, Henryk LU ; Åkerman, Måns LU and Nilbert, Mef LU (2004) In Sarcoma 8(4). p.123-127
Abstract
Purpose: Soft tissue sarcomas (STS) account for less than 1% of all malignancies and constitute a heterogeneous tumor entity in which malignant fibrous histiocytomas (MFH) represent one-third and are characterized by a lack of type-specific differentiation. A defective mismatch repair (MMR) system cause the familial cancer syndrome hereditary non-polyposis colorectal cancer (HNPCC), and since occasional MFH have been described in HNPCC patients we assessed the contribution of defective MMR to the development of MFH.



Methods: MMR status was characterized in a series of 209 histopathologically reviewed MFH. Tissue microarray sections from the tumors were immunohistochemically stained for the MMR proteins MLH1, MSH2 and... (More)
Purpose: Soft tissue sarcomas (STS) account for less than 1% of all malignancies and constitute a heterogeneous tumor entity in which malignant fibrous histiocytomas (MFH) represent one-third and are characterized by a lack of type-specific differentiation. A defective mismatch repair (MMR) system cause the familial cancer syndrome hereditary non-polyposis colorectal cancer (HNPCC), and since occasional MFH have been described in HNPCC patients we assessed the contribution of defective MMR to the development of MFH.



Methods: MMR status was characterized in a series of 209 histopathologically reviewed MFH. Tissue microarray sections from the tumors were immunohistochemically stained for the MMR proteins MLH1, MSH2 and MSH6, and cases with aberrant staining were further characterized for microsatellite instability.



Results and Discussion: Two of the 209 STS–a storiform-pleomorphic MFH and a myxofibrosarcoma–showed concomitant loss of MSH2 and MSH6, but retained staining for MLH1 on both cases. The myxoid tumor also had a microsatellite unstable phenotype. These findings, together with previous observations of defective MMR in pleomorphic STS, indicate that these tumors may be part of the HNPCC-associated tumor spectrum and demonstrate that MMR defects occur in a small subset of STS. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Sarcoma
volume
8
issue
4
pages
123 - 127
publisher
Hindawi Publishing Corporation
ISSN
1357-714X
language
English
LU publication?
yes
id
a6ec7288-dfcf-40ce-b1ff-786040b6e544 (old id 1129340)
alternative location
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2395618/
date added to LUP
2008-06-19 11:21:05
date last changed
2016-06-09 10:38:29
@article{a6ec7288-dfcf-40ce-b1ff-786040b6e544,
  abstract     = {Purpose: Soft tissue sarcomas (STS) account for less than 1% of all malignancies and constitute a heterogeneous tumor entity in which malignant fibrous histiocytomas (MFH) represent one-third and are characterized by a lack of type-specific differentiation. A defective mismatch repair (MMR) system cause the familial cancer syndrome hereditary non-polyposis colorectal cancer (HNPCC), and since occasional MFH have been described in HNPCC patients we assessed the contribution of defective MMR to the development of MFH.<br/><br>
<br/><br>
Methods: MMR status was characterized in a series of 209 histopathologically reviewed MFH. Tissue microarray sections from the tumors were immunohistochemically stained for the MMR proteins MLH1, MSH2 and MSH6, and cases with aberrant staining were further characterized for microsatellite instability.<br/><br>
<br/><br>
Results and Discussion: Two of the 209 STS–a storiform-pleomorphic MFH and a myxofibrosarcoma–showed concomitant loss of MSH2 and MSH6, but retained staining for MLH1 on both cases. The myxoid tumor also had a microsatellite unstable phenotype. These findings, together with previous observations of defective MMR in pleomorphic STS, indicate that these tumors may be part of the HNPCC-associated tumor spectrum and demonstrate that MMR defects occur in a small subset of STS.},
  author       = {Ericson Lindquist, Kajsa and Engellau, Jacob and Persson, Annette and Lindblom, Annika and Domanski, Henryk and Åkerman, Måns and Nilbert, Mef},
  issn         = {1357-714X},
  language     = {eng},
  number       = {4},
  pages        = {123--127},
  publisher    = {Hindawi Publishing Corporation},
  series       = {Sarcoma},
  title        = {Immunohistochemical loss of the DNA mismatch repair proteins MSH2 and MSH6 in malignant fibrous histocytomas},
  volume       = {8},
  year         = {2004},
}