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Profily endometrialni exprese mRNA u vybranych matrix metaloproteinaz a tkanoveho inhibitoru metaloproteinaz-1

Pilka, Radovan LU ; Kudela, M; Eriksson, P and Casslén, Bertil LU (2004) In Ceska gynekologie / Ceska lekarska spolecnost J. Ev. Purkyne 69(6). p.472-476
Abstract
OBJECTIVE: To examine the endometrial expression pattern of messenger RNA (mRNA) for selected matrix metalloproteinases (MMP) and tissue inhibitor of metalloproteinases-1 (TIMP-1). DESIGN: Experimental study. SETTING: Department of Obstetrics and gynecology of the Palacky University Medical School and University Hospital, Olomouc, Czech Republic, Department of Obsterics and Gynecology, University Hospital, Luna, Sweden. METHODS: We studied MMP-1, -3, -7, -10, -11, -12, -13, -14, -16, -26 and TIMP-1 mRNA in 39 normal endometrial samples obtained across the menstrual cycle. Based on histological examination, all specimens were classified according to an ideal 28 day menstrual cycle as early (n=8), mid (n=6) and late (n=7) proliferative... (More)
OBJECTIVE: To examine the endometrial expression pattern of messenger RNA (mRNA) for selected matrix metalloproteinases (MMP) and tissue inhibitor of metalloproteinases-1 (TIMP-1). DESIGN: Experimental study. SETTING: Department of Obstetrics and gynecology of the Palacky University Medical School and University Hospital, Olomouc, Czech Republic, Department of Obsterics and Gynecology, University Hospital, Luna, Sweden. METHODS: We studied MMP-1, -3, -7, -10, -11, -12, -13, -14, -16, -26 and TIMP-1 mRNA in 39 normal endometrial samples obtained across the menstrual cycle. Based on histological examination, all specimens were classified according to an ideal 28 day menstrual cycle as early (n=8), mid (n=6) and late (n=7) proliferative phase, early (n=4), mid (n=4) and late (n=8) secretory phase and menstrual (n=3) phase. mRNA extracted from frozen tissue samples was quantitated using real time PCR. RESULTS: Four distinct patterns of MMP mRNA expression were detected in cycling endometrium. MMP-1, -3, -10, and -12 were expressed predominantly in perimenstrual period, MMP-7 and -11 had highest levels in proliferative phase, MMP-13, -14 and -16 were expressed throughout the cycle and MMP-26 was found to be maximal in periovulatory period. Levels of TIMP-1 mRNA remained unchanged during the cycle. CONCLUSION: The specific endometrial expression profiles of MMPs during menstrual cycle point to their specific biologic roles during the cycle. MMP-26 exhibits a unique expression pattern. (Less)
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alternative title
Endometrial expression profiles of mRNA for selected matrix metalloproteinases and tissue inhibitor of metalloproteinases-1
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Contribution to journal
publication status
published
subject
in
Ceska gynekologie / Ceska lekarska spolecnost J. Ev. Purkyne
volume
69
issue
6
pages
472 - 476
publisher
Ceska Lekarska Spolecnost J. E. Purkyne
external identifiers
  • pmid:15633417
  • scopus:9644278158
ISSN
1210-7832
language
Other
LU publication?
yes
id
ae807576-c7de-4554-87f1-61b73d49f9e4 (old id 1129629)
date added to LUP
2008-06-18 09:31:26
date last changed
2017-01-01 06:48:20
@article{ae807576-c7de-4554-87f1-61b73d49f9e4,
  abstract     = {OBJECTIVE: To examine the endometrial expression pattern of messenger RNA (mRNA) for selected matrix metalloproteinases (MMP) and tissue inhibitor of metalloproteinases-1 (TIMP-1). DESIGN: Experimental study. SETTING: Department of Obstetrics and gynecology of the Palacky University Medical School and University Hospital, Olomouc, Czech Republic, Department of Obsterics and Gynecology, University Hospital, Luna, Sweden. METHODS: We studied MMP-1, -3, -7, -10, -11, -12, -13, -14, -16, -26 and TIMP-1 mRNA in 39 normal endometrial samples obtained across the menstrual cycle. Based on histological examination, all specimens were classified according to an ideal 28 day menstrual cycle as early (n=8), mid (n=6) and late (n=7) proliferative phase, early (n=4), mid (n=4) and late (n=8) secretory phase and menstrual (n=3) phase. mRNA extracted from frozen tissue samples was quantitated using real time PCR. RESULTS: Four distinct patterns of MMP mRNA expression were detected in cycling endometrium. MMP-1, -3, -10, and -12 were expressed predominantly in perimenstrual period, MMP-7 and -11 had highest levels in proliferative phase, MMP-13, -14 and -16 were expressed throughout the cycle and MMP-26 was found to be maximal in periovulatory period. Levels of TIMP-1 mRNA remained unchanged during the cycle. CONCLUSION: The specific endometrial expression profiles of MMPs during menstrual cycle point to their specific biologic roles during the cycle. MMP-26 exhibits a unique expression pattern.},
  author       = {Pilka, Radovan and Kudela, M and Eriksson, P and Casslén, Bertil},
  issn         = {1210-7832},
  language     = {mis},
  number       = {6},
  pages        = {472--476},
  publisher    = {Ceska Lekarska Spolecnost J. E. Purkyne},
  series       = {Ceska gynekologie / Ceska lekarska spolecnost J. Ev. Purkyne},
  title        = {Profily endometrialni exprese mRNA u vybranych matrix metaloproteinaz a tkanoveho inhibitoru metaloproteinaz-1},
  volume       = {69},
  year         = {2004},
}