Advanced

Transgenic rescue demonstrates involvement of the Ian5 gene in T cell development in the rat.

Michalkiewicz, Mieczyslaw; Michalkiewicz, Teresa; Ettinger, Ruth A.; Rutledge, Elizabeth A.; Fuller, Jessica M.; Moralejo, Daniel H.; Van Yserloo, Brian; MacMurray, Armand J.; Kwitek, Anne E. and Jacob, Howard J., et al. (2004) In Physiological Genomics 19(2). p.228-232
Abstract
A single point mutation in a novel immune-associated nucleotide gene 5 (Ian5) coincides with severe T cell lymphopenia in BB rats. We used a transgenic rescue approach in lymphopenic BB-derived congenic F344.lyp/lyp rats to determine whether this mutation is responsible for lymphopenia and to establish the functional importance of this novel gene. A 150-kb P1 artificial chromosome (PAC) transgene harboring a wild-type allele of the rat Ian5 gene restored Ian5 transcript and protein levels, completely rescuing the T cell lymphopenia in the F344.lyp/lyp rats. This successful complementation provides direct functional evidence that the Ian5 gene product is essential for maintaining normal T cell levels. It also demonstrates that transgenic... (More)
A single point mutation in a novel immune-associated nucleotide gene 5 (Ian5) coincides with severe T cell lymphopenia in BB rats. We used a transgenic rescue approach in lymphopenic BB-derived congenic F344.lyp/lyp rats to determine whether this mutation is responsible for lymphopenia and to establish the functional importance of this novel gene. A 150-kb P1 artificial chromosome (PAC) transgene harboring a wild-type allele of the rat Ian5 gene restored Ian5 transcript and protein levels, completely rescuing the T cell lymphopenia in the F344.lyp/lyp rats. This successful complementation provides direct functional evidence that the Ian5 gene product is essential for maintaining normal T cell levels. It also demonstrates that transgenic rescue in the rat is a practical and definitive method for revealing the function of a novel gene. (Less)
Please use this url to cite or link to this publication:
author
, et al. (More)
(Less)
publishing date
type
Contribution to journal
publication status
published
subject
in
Physiological Genomics
volume
19
issue
2
pages
228 - 232
publisher
American Physiological Society
external identifiers
  • scopus:19944386204
ISSN
1094-8341
DOI
10.1152/physiolgenomics.00126.2004
language
English
LU publication?
no
id
4ca489e9-eb11-4204-8748-7520ddab92be (old id 1130253)
date added to LUP
2008-06-17 10:38:38
date last changed
2017-08-27 03:47:12
@article{4ca489e9-eb11-4204-8748-7520ddab92be,
  abstract     = {A single point mutation in a novel immune-associated nucleotide gene 5 (Ian5) coincides with severe T cell lymphopenia in BB rats. We used a transgenic rescue approach in lymphopenic BB-derived congenic F344.lyp/lyp rats to determine whether this mutation is responsible for lymphopenia and to establish the functional importance of this novel gene. A 150-kb P1 artificial chromosome (PAC) transgene harboring a wild-type allele of the rat Ian5 gene restored Ian5 transcript and protein levels, completely rescuing the T cell lymphopenia in the F344.lyp/lyp rats. This successful complementation provides direct functional evidence that the Ian5 gene product is essential for maintaining normal T cell levels. It also demonstrates that transgenic rescue in the rat is a practical and definitive method for revealing the function of a novel gene.},
  author       = {Michalkiewicz, Mieczyslaw and Michalkiewicz, Teresa and Ettinger, Ruth A. and Rutledge, Elizabeth A. and Fuller, Jessica M. and Moralejo, Daniel H. and Van Yserloo, Brian and MacMurray, Armand J. and Kwitek, Anne E. and Jacob, Howard J. and Lander, Eric S. and Lernmark, Åke},
  issn         = {1094-8341},
  language     = {eng},
  number       = {2},
  pages        = {228--232},
  publisher    = {American Physiological Society},
  series       = {Physiological Genomics},
  title        = {Transgenic rescue demonstrates involvement of the Ian5 gene in T cell development in the rat.},
  url          = {http://dx.doi.org/10.1152/physiolgenomics.00126.2004},
  volume       = {19},
  year         = {2004},
}