Advanced

Differential effects of VIP and PACAP on survival of cultured adult rat myenteric neurons.

Sandgren, Katarina LU ; Lin, Zhong and Ekblad, Eva LU (2003) In Regulatory Peptides 111(1-3). p.211-217
Abstract
Our knowledge of neuroprotective factors important for the adult enteric nervous system is poor. Changes in expression of vasoactive intestinal peptide (VIP) and pituitary adenylate cyclase-activating peptide (PACAP) in enteric neurons in response to neuronal injury or colchicine treatment, as well as in intestinal adaptation, have been described. Cultured myenteric neurons increase their expression of VIP; furthermore, culturing myenteric neurons in the presence of VIP enhances neuronal survival. The aims of this study were to evaluate possible changes in PACAP expression in dissociated and cultured myenteric neurons from adult rat small intestine, and to determine the ability of PACAP-38 and PACAP-27 to promote survival of cultured... (More)
Our knowledge of neuroprotective factors important for the adult enteric nervous system is poor. Changes in expression of vasoactive intestinal peptide (VIP) and pituitary adenylate cyclase-activating peptide (PACAP) in enteric neurons in response to neuronal injury or colchicine treatment, as well as in intestinal adaptation, have been described. Cultured myenteric neurons increase their expression of VIP; furthermore, culturing myenteric neurons in the presence of VIP enhances neuronal survival. The aims of this study were to evaluate possible changes in PACAP expression in dissociated and cultured myenteric neurons from adult rat small intestine, and to determine the ability of PACAP-38 and PACAP-27 to promote survival of cultured myenteric neurons, as compared with that of VIP. A marked decrease in the number of surviving neurons was noted during culturing. No difference in neuronal survival was found after culturing in the presence of PACAP-38 or PACAP-27, whereas VIP significantly increased neuronal survival. In contrast to the marked increase noted in the number of VIP-expressing neurons, culturing caused no change in the number of PACAP-expressing myenteric neurons. We were thus able to demonstrate that VIP, but not PACAP, promoted survival of myenteric neurons in culture. This suggests the presence of a VIP-specific receptor mediating neuroprotection in adult myenteric neurons. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Neuroprotection, Enteric nervous system, VIP-specific receptor, Neuronal survival, Neurotransmitter expression
in
Regulatory Peptides
volume
111
issue
1-3
pages
211 - 217
publisher
Elsevier
external identifiers
  • wos:000181621700029
  • pmid:12609771
  • scopus:0037471376
ISSN
1873-1686
DOI
language
English
LU publication?
yes
id
47f56fea-34aa-490d-a560-4bd02f353b22 (old id 113074)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12609771&dopt=Abstract
date added to LUP
2007-06-26 12:38:21
date last changed
2018-05-29 10:01:24
@article{47f56fea-34aa-490d-a560-4bd02f353b22,
  abstract     = {Our knowledge of neuroprotective factors important for the adult enteric nervous system is poor. Changes in expression of vasoactive intestinal peptide (VIP) and pituitary adenylate cyclase-activating peptide (PACAP) in enteric neurons in response to neuronal injury or colchicine treatment, as well as in intestinal adaptation, have been described. Cultured myenteric neurons increase their expression of VIP; furthermore, culturing myenteric neurons in the presence of VIP enhances neuronal survival. The aims of this study were to evaluate possible changes in PACAP expression in dissociated and cultured myenteric neurons from adult rat small intestine, and to determine the ability of PACAP-38 and PACAP-27 to promote survival of cultured myenteric neurons, as compared with that of VIP. A marked decrease in the number of surviving neurons was noted during culturing. No difference in neuronal survival was found after culturing in the presence of PACAP-38 or PACAP-27, whereas VIP significantly increased neuronal survival. In contrast to the marked increase noted in the number of VIP-expressing neurons, culturing caused no change in the number of PACAP-expressing myenteric neurons. We were thus able to demonstrate that VIP, but not PACAP, promoted survival of myenteric neurons in culture. This suggests the presence of a VIP-specific receptor mediating neuroprotection in adult myenteric neurons.},
  author       = {Sandgren, Katarina and Lin, Zhong and Ekblad, Eva},
  issn         = {1873-1686},
  keyword      = {Neuroprotection,Enteric nervous system,VIP-specific receptor,Neuronal survival,Neurotransmitter expression},
  language     = {eng},
  number       = {1-3},
  pages        = {211--217},
  publisher    = {Elsevier},
  series       = {Regulatory Peptides},
  title        = {Differential effects of VIP and PACAP on survival of cultured adult rat myenteric neurons.},
  url          = {http://dx.doi.org/},
  volume       = {111},
  year         = {2003},
}