Islet amyloid polypeptide inhibits glucagon release and exerts a dual action on insulin release from isolated islets.
(2003) In Regulatory Peptides 111(1-3). p.55-60- Abstract
- We have studied the influence of a wide concentration range of islet amyloid polypeptide (IAPP) on both glucagon and insulin release stimulated by various types of secretagogues. In an islet incubation medium devoid of glucose, the rate of glucagon release being high, we observed a marked suppressive action by low concentrations of IAPP, 10−10 and 10−8 M, on glucagon release. Similarly, glucagon release stimulated by Image-arginine, the cholinergic agonist carbachol, or the phosphodiesterase inhibitor isobutylmethyl xanthine (IBMX), an activator of the cyclic AMP system, was inhibited by IAPP in the 10−10 and 10−8 M concentration range. Moreover, basal glucagon release at 7 and 10 mM glucose was suppressed by IAPP. In contrast, IAPP... (More)
- We have studied the influence of a wide concentration range of islet amyloid polypeptide (IAPP) on both glucagon and insulin release stimulated by various types of secretagogues. In an islet incubation medium devoid of glucose, the rate of glucagon release being high, we observed a marked suppressive action by low concentrations of IAPP, 10−10 and 10−8 M, on glucagon release. Similarly, glucagon release stimulated by Image-arginine, the cholinergic agonist carbachol, or the phosphodiesterase inhibitor isobutylmethyl xanthine (IBMX), an activator of the cyclic AMP system, was inhibited by IAPP in the 10−10 and 10−8 M concentration range. Moreover, basal glucagon release at 7 and 10 mM glucose was suppressed by IAPP. In contrast, IAPP exerted a dual action on insulin release. Hence, low concentrations of IAPP brought about a modest increase of basal insulin secretion at 7 mM glucose and also of insulin release stimulated by carbachol. High concentrations of IAPP, however, inhibited insulin release stimulated by glucose (10 and 16.7 mM), IBMX, carbachol and Image-arginine. In conclusion, our data suggest that IAPP has complex effects on islet hormone secretion serving as an inhibitor of glucagon release and having a dual action on insulin secretion exerting mainly a negative feedback on stimulated and a positive feedback on basal insulin release. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/113079
- author
- Åkesson, Björn LU ; Panagiotidis, Georgios ; Westermark, Per and Lundquist, Ingmar LU
- organization
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Phosphodiesterase inhibition, Image-Arginine, slet secretory mechanisms, Glucose, Cholinergic agonist
- in
- Regulatory Peptides
- volume
- 111
- issue
- 1-3
- pages
- 55 - 60
- publisher
- Elsevier
- external identifiers
-
- pmid:12609749
- wos:000181621700007
- scopus:0037471369
- ISSN
- 1873-1686
- DOI
- 10.1016/S0167-0115(02)00252-5
- language
- English
- LU publication?
- yes
- id
- ac41c821-9529-4c2e-8f2a-3620fca1fbb7 (old id 113079)
- alternative location
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12609749&dopt=Abstract
- date added to LUP
- 2016-04-01 12:23:46
- date last changed
- 2022-03-29 00:18:58
@article{ac41c821-9529-4c2e-8f2a-3620fca1fbb7,
abstract = {{We have studied the influence of a wide concentration range of islet amyloid polypeptide (IAPP) on both glucagon and insulin release stimulated by various types of secretagogues. In an islet incubation medium devoid of glucose, the rate of glucagon release being high, we observed a marked suppressive action by low concentrations of IAPP, 10−10 and 10−8 M, on glucagon release. Similarly, glucagon release stimulated by Image-arginine, the cholinergic agonist carbachol, or the phosphodiesterase inhibitor isobutylmethyl xanthine (IBMX), an activator of the cyclic AMP system, was inhibited by IAPP in the 10−10 and 10−8 M concentration range. Moreover, basal glucagon release at 7 and 10 mM glucose was suppressed by IAPP. In contrast, IAPP exerted a dual action on insulin release. Hence, low concentrations of IAPP brought about a modest increase of basal insulin secretion at 7 mM glucose and also of insulin release stimulated by carbachol. High concentrations of IAPP, however, inhibited insulin release stimulated by glucose (10 and 16.7 mM), IBMX, carbachol and Image-arginine. In conclusion, our data suggest that IAPP has complex effects on islet hormone secretion serving as an inhibitor of glucagon release and having a dual action on insulin secretion exerting mainly a negative feedback on stimulated and a positive feedback on basal insulin release.}},
author = {{Åkesson, Björn and Panagiotidis, Georgios and Westermark, Per and Lundquist, Ingmar}},
issn = {{1873-1686}},
keywords = {{Phosphodiesterase inhibition; Image-Arginine; slet secretory mechanisms; Glucose; Cholinergic agonist}},
language = {{eng}},
number = {{1-3}},
pages = {{55--60}},
publisher = {{Elsevier}},
series = {{Regulatory Peptides}},
title = {{Islet amyloid polypeptide inhibits glucagon release and exerts a dual action on insulin release from isolated islets.}},
url = {{http://dx.doi.org/10.1016/S0167-0115(02)00252-5}},
doi = {{10.1016/S0167-0115(02)00252-5}},
volume = {{111}},
year = {{2003}},
}