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IgE-reactivity to seven Malassezia species.

Zargari, A.; Midgley, G.; Bäck, Ove LU ; Johansson, S. G. O. and Scheynius, A. (2003) In Allergy 58(4). p.306-311
Abstract
Background: Malassezia yeasts play a role in the pathogenesis of atopic eczema/dermatitis syndrome (AEDS). The revised genus Malassezia includes several species which all are natural habitants of the human skin. In this study, we evaluated the presence of immunoglobulin E (IgE) antibodies to different Malassezia spp. in AEDS patients to allow optimization of the characterization of the IgE antibody profile of IgE-associated AEDS.



Methods: Ninety-six adult patients, with a clinical diagnosis of AEDS, were included in the study. Seventeen of the patients had IgE antibodies to M. sympodialis, ATCC 42132 (m70 ImmunoCAP, Pharmacia, Diagnostic AB, Uppsala, Sweden). The IgE antibodies to seven Malassezia spp. were measured and... (More)
Background: Malassezia yeasts play a role in the pathogenesis of atopic eczema/dermatitis syndrome (AEDS). The revised genus Malassezia includes several species which all are natural habitants of the human skin. In this study, we evaluated the presence of immunoglobulin E (IgE) antibodies to different Malassezia spp. in AEDS patients to allow optimization of the characterization of the IgE antibody profile of IgE-associated AEDS.



Methods: Ninety-six adult patients, with a clinical diagnosis of AEDS, were included in the study. Seventeen of the patients had IgE antibodies to M. sympodialis, ATCC 42132 (m70 ImmunoCAP, Pharmacia, Diagnostic AB, Uppsala, Sweden). The IgE antibodies to seven Malassezia spp. were measured and inhibition immunoblotting was performed to investigate whether M. sympodialis contains all the allergen components present in the other Malassezia spp.



Results: Twenty per cent of 79 AEDS patients with a negative m70 ImmunoCAP test had IgE antibodies to at least one of the other six Malassezia spp. tested. Our inhibition studies indicated that Malassezia spp. to a great extent, share allergenic determinants. However, Malassezia species also contained species-specific allergens.



Conclusion: The use of only one species of Malassezia is not sufficient to detect all patients IgE sensitized to Malassezia. To obtain an optimal allergen preparation both common allergenic components as well as species-specific allergens have to be considered. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Allergy
volume
58
issue
4
pages
306 - 311
publisher
Wiley-Blackwell
external identifiers
  • wos:000182324000007
  • pmid:12708978
  • scopus:0038333353
ISSN
1398-9995
DOI
10.1034/j.1398-9995.2003.00082.x
language
English
LU publication?
yes
id
4a66fedb-e7b0-475c-94e9-4e469012a8a2 (old id 113234)
alternative location
http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=Retrieve&db=PubMed&list_uids=12708978&dopt=Abstract
date added to LUP
2007-07-03 09:32:02
date last changed
2018-05-29 12:18:31
@article{4a66fedb-e7b0-475c-94e9-4e469012a8a2,
  abstract     = {Background: Malassezia yeasts play a role in the pathogenesis of atopic eczema/dermatitis syndrome (AEDS). The revised genus Malassezia includes several species which all are natural habitants of the human skin. In this study, we evaluated the presence of immunoglobulin E (IgE) antibodies to different Malassezia spp. in AEDS patients to allow optimization of the characterization of the IgE antibody profile of IgE-associated AEDS.<br/><br>
<br/><br>
Methods: Ninety-six adult patients, with a clinical diagnosis of AEDS, were included in the study. Seventeen of the patients had IgE antibodies to M. sympodialis, ATCC 42132 (m70 ImmunoCAP, Pharmacia, Diagnostic AB, Uppsala, Sweden). The IgE antibodies to seven Malassezia spp. were measured and inhibition immunoblotting was performed to investigate whether M. sympodialis contains all the allergen components present in the other Malassezia spp.<br/><br>
<br/><br>
Results: Twenty per cent of 79 AEDS patients with a negative m70 ImmunoCAP test had IgE antibodies to at least one of the other six Malassezia spp. tested. Our inhibition studies indicated that Malassezia spp. to a great extent, share allergenic determinants. However, Malassezia species also contained species-specific allergens.<br/><br>
<br/><br>
Conclusion: The use of only one species of Malassezia is not sufficient to detect all patients IgE sensitized to Malassezia. To obtain an optimal allergen preparation both common allergenic components as well as species-specific allergens have to be considered.},
  author       = {Zargari, A. and Midgley, G. and Bäck, Ove and Johansson, S. G. O. and Scheynius, A.},
  issn         = {1398-9995},
  language     = {eng},
  number       = {4},
  pages        = {306--311},
  publisher    = {Wiley-Blackwell},
  series       = {Allergy},
  title        = {IgE-reactivity to seven Malassezia species.},
  url          = {http://dx.doi.org/10.1034/j.1398-9995.2003.00082.x},
  volume       = {58},
  year         = {2003},
}