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MMP-26 mRNA and estrogen receptor alpha co-expression in normal and pathological endometrium

Pilka, R; Kudela, M; Eriksson, P and Casslén, Bertil LU (2005) In Ceska gynekologie / Ceska lekarska spolecnost J. Ev. Purkyne 70(1). p.56-56
Abstract
OBJECTIVE: To examine the expression pattern of matrix metalloproteinase-26 (MMP-26) mRNA and estrogen receptor-alpha (ER alpha) in normal, hyperplastic, premalignant and malignant endometrial tissue. DESIGN: Experimental study. SETTING: Department of Obstetrics and Gynecology of the Palacky University Medical School and University Hospital, Olomouc, Czech Republic, Department of Obstetrics and Gynecology, University Hospital, Lund, Sweden, Atherosclerosis Research Unit, King Gustav V Research Institute, Karolinska Hospital, Stockholm, Sweden. METHODS: We studied MMP-26 mRNA and ER alpha in 36 normal, 7 hyperplastic, 6 premalignant and 19 malignant endometrial samples. Based on histological examination, all normal specimens were classified... (More)
OBJECTIVE: To examine the expression pattern of matrix metalloproteinase-26 (MMP-26) mRNA and estrogen receptor-alpha (ER alpha) in normal, hyperplastic, premalignant and malignant endometrial tissue. DESIGN: Experimental study. SETTING: Department of Obstetrics and Gynecology of the Palacky University Medical School and University Hospital, Olomouc, Czech Republic, Department of Obstetrics and Gynecology, University Hospital, Lund, Sweden, Atherosclerosis Research Unit, King Gustav V Research Institute, Karolinska Hospital, Stockholm, Sweden. METHODS: We studied MMP-26 mRNA and ER alpha in 36 normal, 7 hyperplastic, 6 premalignant and 19 malignant endometrial samples. Based on histological examination, all normal specimens were classified according to an ideal 28 day menstrual cycle as early, mid, and late proliferative phase, early, mid and late secretory phase and menstrual phase. Samples with hyperplasia were classified as simple or complex. Premalignant samples were represented by complex hyperplasia with atypia. Malignant samples were histologically classified as well, intermediately and poorly differentiated, respectively. Specimens were analyzed using in situ hybridization and real time PCR. ER alpha was localized by immunohistochemistry. RESULTS: Epithelial MMP-26 mRNA expression was highest in the early secretory phase and in endometrial hyperplasia. Expression levels were low in the late secretory and menstrual phase and in malignant samples decreased gradually with dedifferentiation. Expression pattern of MMP-26 mRNA in normal, hyperplastic, premalignant and malignant endometrial tissue strongly co-variated with that of ER alpha. CONCLUSION: Co-expression of MMP-26 and ER alpha in normal and pathological endometrial tissue suggests possible regulation of MMP-26 gene by estrogen. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
keywords
ndometrium, MMP-26, mRNA, regulation, ER
in
Ceska gynekologie / Ceska lekarska spolecnost J. Ev. Purkyne
volume
70
issue
1
pages
56 - 56
publisher
Ceska Lekarska Spolecnost J. E. Purkyne
external identifiers
  • pmid:15779297
  • scopus:13944250002
ISSN
1210-7832
language
Other
LU publication?
yes
id
783adbbb-0ff0-459c-86c6-7897c112ea10 (old id 1132717)
date added to LUP
2008-06-27 09:04:05
date last changed
2017-01-01 07:10:58
@article{783adbbb-0ff0-459c-86c6-7897c112ea10,
  abstract     = {OBJECTIVE: To examine the expression pattern of matrix metalloproteinase-26 (MMP-26) mRNA and estrogen receptor-alpha (ER alpha) in normal, hyperplastic, premalignant and malignant endometrial tissue. DESIGN: Experimental study. SETTING: Department of Obstetrics and Gynecology of the Palacky University Medical School and University Hospital, Olomouc, Czech Republic, Department of Obstetrics and Gynecology, University Hospital, Lund, Sweden, Atherosclerosis Research Unit, King Gustav V Research Institute, Karolinska Hospital, Stockholm, Sweden. METHODS: We studied MMP-26 mRNA and ER alpha in 36 normal, 7 hyperplastic, 6 premalignant and 19 malignant endometrial samples. Based on histological examination, all normal specimens were classified according to an ideal 28 day menstrual cycle as early, mid, and late proliferative phase, early, mid and late secretory phase and menstrual phase. Samples with hyperplasia were classified as simple or complex. Premalignant samples were represented by complex hyperplasia with atypia. Malignant samples were histologically classified as well, intermediately and poorly differentiated, respectively. Specimens were analyzed using in situ hybridization and real time PCR. ER alpha was localized by immunohistochemistry. RESULTS: Epithelial MMP-26 mRNA expression was highest in the early secretory phase and in endometrial hyperplasia. Expression levels were low in the late secretory and menstrual phase and in malignant samples decreased gradually with dedifferentiation. Expression pattern of MMP-26 mRNA in normal, hyperplastic, premalignant and malignant endometrial tissue strongly co-variated with that of ER alpha. CONCLUSION: Co-expression of MMP-26 and ER alpha in normal and pathological endometrial tissue suggests possible regulation of MMP-26 gene by estrogen.},
  author       = {Pilka, R and Kudela, M and Eriksson, P and Casslén, Bertil},
  issn         = {1210-7832},
  keyword      = {ndometrium,MMP-26,mRNA,regulation,ER},
  language     = {mis},
  number       = {1},
  pages        = {56--56},
  publisher    = {Ceska Lekarska Spolecnost J. E. Purkyne},
  series       = {Ceska gynekologie / Ceska lekarska spolecnost J. Ev. Purkyne},
  title        = {MMP-26 mRNA and estrogen receptor alpha co-expression in normal and pathological endometrium},
  volume       = {70},
  year         = {2005},
}