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Analiza rokowniczego znaczenia mutacji genu TP53 u chorych na niedrobnokomorkowego raka pluca

Szymanowska, Amelia; Jassem, Ewa; Dziadziuszko, Rafal; Skrzypski, Marcin; Kobierska-Gulida, Grazyna; Holm, Karolina LU ; Borg, Åke LU ; Rzyman, Witold; Limon, Janusz and Jassem, Jacek (2005) In Pneumonologia i Alergologia Polska 73(3). p.264-269
Abstract
The aim of this study was to assess the frequency and prognostic value of TP53 gene somatic mutations in non-small cell lung cancer. The study group included 240 NSCLC patients who underwent pulmonary resection at the Department of Thoracic Surgery, Medical University of Gdansk. Tumour samples were evaluated for the presence of TP53 gene mutations in exons 5-8. In 157 cases SSCP method was used as a screening followed by sequencing of positive samples. In the remaining 83 patients mutations were analysed by direct sequencing. A total of 76 mutations (32%) were found, of those a missense type was dominant (67%), followed by silent and null type mutations (14% and 10%, respectively). There was no correlation between mutations and clinical... (More)
The aim of this study was to assess the frequency and prognostic value of TP53 gene somatic mutations in non-small cell lung cancer. The study group included 240 NSCLC patients who underwent pulmonary resection at the Department of Thoracic Surgery, Medical University of Gdansk. Tumour samples were evaluated for the presence of TP53 gene mutations in exons 5-8. In 157 cases SSCP method was used as a screening followed by sequencing of positive samples. In the remaining 83 patients mutations were analysed by direct sequencing. A total of 76 mutations (32%) were found, of those a missense type was dominant (67%), followed by silent and null type mutations (14% and 10%, respectively). There was no correlation between mutations and clinical characteristics, including age, sex, histological subtype, differentiation, tumour size, lymph node metastases, pTNM stage and smoking status. A multivariate Cox analysis demonstrated that tumour differentiation and pTNM stage were independent prognostic factors, whereas TP53 gene mutations were not. The results of this study indicate that TP53 gene mutations in NSCLC patients are not correlated with clinical characteristics and have no impact on survival. (Less)
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author
organization
alternative title
Analysis of prognostic value of TP53 gene mutations in non-small cell lung cancer
publishing date
type
Contribution to journal
publication status
published
subject
in
Pneumonologia i Alergologia Polska
volume
73
issue
3
pages
264 - 269
publisher
Wydawnictwo Viamedica SPZOO
external identifiers
  • pmid:16989164
  • scopus:33645971611
ISSN
0867-7077
language
Polish
LU publication?
yes
id
ed966b14-5cfe-4369-ae23-6bf5f3eecef4 (old id 1132943)
date added to LUP
2008-06-23 14:50:39
date last changed
2017-01-01 07:28:37
@article{ed966b14-5cfe-4369-ae23-6bf5f3eecef4,
  abstract     = {The aim of this study was to assess the frequency and prognostic value of TP53 gene somatic mutations in non-small cell lung cancer. The study group included 240 NSCLC patients who underwent pulmonary resection at the Department of Thoracic Surgery, Medical University of Gdansk. Tumour samples were evaluated for the presence of TP53 gene mutations in exons 5-8. In 157 cases SSCP method was used as a screening followed by sequencing of positive samples. In the remaining 83 patients mutations were analysed by direct sequencing. A total of 76 mutations (32%) were found, of those a missense type was dominant (67%), followed by silent and null type mutations (14% and 10%, respectively). There was no correlation between mutations and clinical characteristics, including age, sex, histological subtype, differentiation, tumour size, lymph node metastases, pTNM stage and smoking status. A multivariate Cox analysis demonstrated that tumour differentiation and pTNM stage were independent prognostic factors, whereas TP53 gene mutations were not. The results of this study indicate that TP53 gene mutations in NSCLC patients are not correlated with clinical characteristics and have no impact on survival.},
  author       = {Szymanowska, Amelia and Jassem, Ewa and Dziadziuszko, Rafal and Skrzypski, Marcin and Kobierska-Gulida, Grazyna and Holm, Karolina and Borg, Åke and Rzyman, Witold and Limon, Janusz and Jassem, Jacek},
  issn         = {0867-7077},
  language     = {pol},
  number       = {3},
  pages        = {264--269},
  publisher    = {Wydawnictwo Viamedica SPZOO},
  series       = {Pneumonologia i Alergologia Polska},
  title        = {Analiza rokowniczego znaczenia mutacji genu TP53 u chorych na niedrobnokomorkowego raka pluca},
  volume       = {73},
  year         = {2005},
}