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Assessment of in vivo MR imaging compared to physical sections in vitro--a quantitative study of brain volumes using stereology

Jelsing, Jacob; Rostrup, Egill; Markenroth Bloch, Karin LU ; Paulson, Olaf B; Gundersen, Hans Jorgen G; Hemmingsen, Ralf and Pakkenberg, Bente (2005) In NeuroImage 26(1). p.57-65
Abstract
The object of the present study was to compare stereological estimates of brain volumes obtained in vivo by magnetic resonance imaging (MRI) to corresponding volumes from physical sections in vitro. Brains of ten domestic pigs were imaged using a 3-T scanner. The volumes of different brain compartments were obtained from MR images by two observers and from physical sections using the Cavalieri estimator in combination with point counting. Paired t tests revealed no significant differences between the two methods for any of the five compartments considered, except for the basal gray compartment. However, although intraobserver difference of MRI estimates was acceptable, the interobserver difference was not. A statistical highly significant... (More)
The object of the present study was to compare stereological estimates of brain volumes obtained in vivo by magnetic resonance imaging (MRI) to corresponding volumes from physical sections in vitro. Brains of ten domestic pigs were imaged using a 3-T scanner. The volumes of different brain compartments were obtained from MR images by two observers and from physical sections using the Cavalieri estimator in combination with point counting. Paired t tests revealed no significant differences between the two methods for any of the five compartments considered, except for the basal gray compartment. However, although intraobserver difference of MRI estimates was acceptable, the interobserver difference was not. A statistical highly significant difference of 11-41% was observed between observers for volume estimates of all compartments considered. The study demonstrates that quantitative MRI is susceptible to observer dependent interpretation of images. (Less)
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author
publishing date
type
Contribution to journal
publication status
published
in
NeuroImage
volume
26
issue
1
pages
57 - 65
publisher
Elsevier
external identifiers
  • pmid:15862205
  • scopus:18044368271
ISSN
1095-9572
DOI
10.1016/j.neuroimage.2005.01.005
language
English
LU publication?
no
id
bd73f643-13ff-4800-83c9-2c2d9915536c (old id 1132989)
date added to LUP
2008-06-23 12:51:40
date last changed
2017-03-14 08:27:56
@article{bd73f643-13ff-4800-83c9-2c2d9915536c,
  abstract     = {The object of the present study was to compare stereological estimates of brain volumes obtained in vivo by magnetic resonance imaging (MRI) to corresponding volumes from physical sections in vitro. Brains of ten domestic pigs were imaged using a 3-T scanner. The volumes of different brain compartments were obtained from MR images by two observers and from physical sections using the Cavalieri estimator in combination with point counting. Paired t tests revealed no significant differences between the two methods for any of the five compartments considered, except for the basal gray compartment. However, although intraobserver difference of MRI estimates was acceptable, the interobserver difference was not. A statistical highly significant difference of 11-41% was observed between observers for volume estimates of all compartments considered. The study demonstrates that quantitative MRI is susceptible to observer dependent interpretation of images.},
  author       = {Jelsing, Jacob and Rostrup, Egill and Markenroth Bloch, Karin and Paulson, Olaf B and Gundersen, Hans Jorgen G and Hemmingsen, Ralf and Pakkenberg, Bente},
  issn         = {1095-9572},
  language     = {eng},
  number       = {1},
  pages        = {57--65},
  publisher    = {Elsevier},
  series       = {NeuroImage},
  title        = {Assessment of in vivo MR imaging compared to physical sections in vitro--a quantitative study of brain volumes using stereology},
  url          = {http://dx.doi.org/10.1016/j.neuroimage.2005.01.005},
  volume       = {26},
  year         = {2005},
}