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Interactions of the extracellular matrix proteoglycans decorin and biglycan with C1q and collectins

Groeneveld, Tom LU ; Oroszlan, Melinda; Owens, Rick T; Faber-Krol, Maria C; Bakker, Astrid C; Arlaud, Gerard J; McQuillan, David J; Kishore, Uday; Daha, Mohamed R and Roos, Anja (2005) In Journal of Immunology 175(7). p.4715-4723
Abstract
Decorin and biglycan are closely related abundant extracellular matrix proteoglycans that have been shown to bind to C1q. Given the overall structural similarities between C1q and mannose-binding lectin (MBL), the two key recognition molecules of the classical and the lectin complement pathways, respectively, we have examined functional consequences of the interaction of C1q and MBL with decorin and biglycan. Recombinant forms of human decorin and biglycan bound C1q via both collagen and globular domains and inhibited the classical pathway. Decorin also bound C1 without activating complement. Furthermore, decorin and biglycan bound efficiently to MBL, but only biglycan could inhibit activation of the lectin pathway. Other members of the... (More)
Decorin and biglycan are closely related abundant extracellular matrix proteoglycans that have been shown to bind to C1q. Given the overall structural similarities between C1q and mannose-binding lectin (MBL), the two key recognition molecules of the classical and the lectin complement pathways, respectively, we have examined functional consequences of the interaction of C1q and MBL with decorin and biglycan. Recombinant forms of human decorin and biglycan bound C1q via both collagen and globular domains and inhibited the classical pathway. Decorin also bound C1 without activating complement. Furthermore, decorin and biglycan bound efficiently to MBL, but only biglycan could inhibit activation of the lectin pathway. Other members of the collectin family, including human surfactant protein D, bovine collectin-43, and conglutinin also showed binding to decorin and biglycan. Decorin and biglycan strongly inhibited C1q binding to human endothelial cells and U937 cells, and biglycan suppressed C1q-induced MCP-1 and IL-8 production by human endothelial cells. In conclusion, decorin and biglycan act as inhibitors of activation of the complement cascade, cellular interactions, and proinflammatory cytokine production mediated by C1q. These two proteoglycans are likely to down-regulate proinflammatory effects mediated by C1q, and possibly also the collectins, at the tissue level. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Immunology
volume
175
issue
7
pages
4715 - 4723
publisher
American Association of Immunologists
external identifiers
  • pmid:16177119
  • scopus:25444512040
ISSN
1550-6606
language
English
LU publication?
yes
id
799f31f4-6dde-41a1-a4fd-ff7c038d4d88 (old id 1133789)
alternative location
http://www.jimmunol.org/cgi/reprint/175/7/4715
date added to LUP
2008-06-17 12:42:34
date last changed
2017-10-22 04:47:20
@article{799f31f4-6dde-41a1-a4fd-ff7c038d4d88,
  abstract     = {Decorin and biglycan are closely related abundant extracellular matrix proteoglycans that have been shown to bind to C1q. Given the overall structural similarities between C1q and mannose-binding lectin (MBL), the two key recognition molecules of the classical and the lectin complement pathways, respectively, we have examined functional consequences of the interaction of C1q and MBL with decorin and biglycan. Recombinant forms of human decorin and biglycan bound C1q via both collagen and globular domains and inhibited the classical pathway. Decorin also bound C1 without activating complement. Furthermore, decorin and biglycan bound efficiently to MBL, but only biglycan could inhibit activation of the lectin pathway. Other members of the collectin family, including human surfactant protein D, bovine collectin-43, and conglutinin also showed binding to decorin and biglycan. Decorin and biglycan strongly inhibited C1q binding to human endothelial cells and U937 cells, and biglycan suppressed C1q-induced MCP-1 and IL-8 production by human endothelial cells. In conclusion, decorin and biglycan act as inhibitors of activation of the complement cascade, cellular interactions, and proinflammatory cytokine production mediated by C1q. These two proteoglycans are likely to down-regulate proinflammatory effects mediated by C1q, and possibly also the collectins, at the tissue level.},
  author       = {Groeneveld, Tom and Oroszlan, Melinda and Owens, Rick T and Faber-Krol, Maria C and Bakker, Astrid C and Arlaud, Gerard J and McQuillan, David J and Kishore, Uday and Daha, Mohamed R and Roos, Anja},
  issn         = {1550-6606},
  language     = {eng},
  number       = {7},
  pages        = {4715--4723},
  publisher    = {American Association of Immunologists},
  series       = {Journal of Immunology},
  title        = {Interactions of the extracellular matrix proteoglycans decorin and biglycan with C1q and collectins},
  volume       = {175},
  year         = {2005},
}