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Quantitative evaluation of HIV and SIV co-receptor use with GHOST(3) cell assay.

Vödrös, Dalma LU and Fenyö, Eva Maria LU (2005) In Human Retrovirus Protocols 2. p.333-342
Abstract
An assay has been established for quantitative evaluation of lentivirus coreceptor use with the help of GHOST(3) cells. GHOST(3) cells were derived from the human osteosarcoma cell line, HOS, and have been engineered to stably express CD4 and one or another of the chemokine receptors CCR3, CCR5, CXCR4, CXCR6/STRL33/Bonzo, or the orphan receptor GPR15/BOB. The indicator cell line carries the HIV-2 long terminal repeat-driven green fluorescence protein (GFP) gene, which becomes activated upon infection with HIV or SIV. Viral entry is followed by Tat activation of transcription and GFP becomes expressed. Infected cells can be detected as early as 2 or 3 d after infection by simple fluorescence microscopic observation. The simplicity of the... (More)
An assay has been established for quantitative evaluation of lentivirus coreceptor use with the help of GHOST(3) cells. GHOST(3) cells were derived from the human osteosarcoma cell line, HOS, and have been engineered to stably express CD4 and one or another of the chemokine receptors CCR3, CCR5, CXCR4, CXCR6/STRL33/Bonzo, or the orphan receptor GPR15/BOB. The indicator cell line carries the HIV-2 long terminal repeat-driven green fluorescence protein (GFP) gene, which becomes activated upon infection with HIV or SIV. Viral entry is followed by Tat activation of transcription and GFP becomes expressed. Infected cells can be detected as early as 2 or 3 d after infection by simple fluorescence microscopic observation. The simplicity of the GHOST(3) cell system makes it particularly suitable for screening of a large number of isolates. In addition, the efficiency of co-receptor use can be accurately quantitated with flow cytometric analysis. Thus, the most efficiently used co-receptor of multitropic isolates can be determined. It is also possible to sensitively determine co-receptor switch of sequential isolates from the same individual. (Less)
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author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
SIV, HIV, quantitative assay, co-receptor use, GFP, GHOST(3)
in
Human Retrovirus Protocols
editor
Zhu, Tuofu and
volume
2
pages
333 - 342
publisher
Humana Press
external identifiers
  • scopus:24944451573
ISSN
1064-3745
1940-6029
ISBN
978-1-58829-495-1
DOI
10.1385/1-59259-907-9:333
language
English
LU publication?
yes
id
290399a8-885d-4f75-ba27-1dfb9aab1995 (old id 1134153)
date added to LUP
2008-06-18 13:12:00
date last changed
2017-08-27 03:59:31
@inbook{290399a8-885d-4f75-ba27-1dfb9aab1995,
  abstract     = {An assay has been established for quantitative evaluation of lentivirus coreceptor use with the help of GHOST(3) cells. GHOST(3) cells were derived from the human osteosarcoma cell line, HOS, and have been engineered to stably express CD4 and one or another of the chemokine receptors CCR3, CCR5, CXCR4, CXCR6/STRL33/Bonzo, or the orphan receptor GPR15/BOB. The indicator cell line carries the HIV-2 long terminal repeat-driven green fluorescence protein (GFP) gene, which becomes activated upon infection with HIV or SIV. Viral entry is followed by Tat activation of transcription and GFP becomes expressed. Infected cells can be detected as early as 2 or 3 d after infection by simple fluorescence microscopic observation. The simplicity of the GHOST(3) cell system makes it particularly suitable for screening of a large number of isolates. In addition, the efficiency of co-receptor use can be accurately quantitated with flow cytometric analysis. Thus, the most efficiently used co-receptor of multitropic isolates can be determined. It is also possible to sensitively determine co-receptor switch of sequential isolates from the same individual.},
  author       = {Vödrös, Dalma and Fenyö, Eva Maria},
  editor       = {Zhu, Tuofu},
  isbn         = {978-1-58829-495-1},
  issn         = {1064-3745},
  keyword      = {SIV,HIV,quantitative assay,co-receptor use,GFP,GHOST(3)},
  language     = {eng},
  pages        = {333--342},
  publisher    = {Humana Press},
  series       = {Human Retrovirus Protocols},
  title        = {Quantitative evaluation of HIV and SIV co-receptor use with GHOST(3) cell assay.},
  url          = {http://dx.doi.org/10.1385/1-59259-907-9:333},
  volume       = {2},
  year         = {2005},
}