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A dataset of human cornea proteins identified by Peptide mass fingerprinting and tandem mass spectrometry

Karring, Henrik LU ; Thogersen, Ida B; Klintworth, Gordon K; Moller-Pedersen, Torben and Enghild, Jan J (2005) In Molecular & Cellular Proteomics 4(9). p.1406-1408
Abstract
Diseases of the cornea are extremely common and cause severe visual impairment worldwide. To explore the basic molecular mechanisms involved in corneal health and disease, the present study characterizes the proteome of the normal human cornea. All proteins were extracted from the central 7-mm region of 12 normal human donor corneas containing all layers: epithelium, Bowman's layer, stroma, Descemet's membrane, and endothelium. Proteins were fractionated and identified using two different procedures: (i) two-dimensional gel electrophoresis and protein identification by MALDI-MS and (ii) strong cation exchange or one-dimensional SDS gel electrophoresis followed by LC-MS/MS. All together, 141 distinct proteins were identified of which 99 had... (More)
Diseases of the cornea are extremely common and cause severe visual impairment worldwide. To explore the basic molecular mechanisms involved in corneal health and disease, the present study characterizes the proteome of the normal human cornea. All proteins were extracted from the central 7-mm region of 12 normal human donor corneas containing all layers: epithelium, Bowman's layer, stroma, Descemet's membrane, and endothelium. Proteins were fractionated and identified using two different procedures: (i) two-dimensional gel electrophoresis and protein identification by MALDI-MS and (ii) strong cation exchange or one-dimensional SDS gel electrophoresis followed by LC-MS/MS. All together, 141 distinct proteins were identified of which 99 had not previously been identified in any mammalian corneas by direct protein identification methods. The characterized proteins are involved in many processes including antiangiogenesis, antimicrobial defense, protection from and transport of heme and iron, tissue protection against UV radiation and oxidative stress, cell metabolism, and maintenance of intracellular and extracellular structures and stability. This proteome study of the healthy human cornea provides a basis for further analysis of corneal diseases and the design of bioengineered corneas. (Less)
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author
publishing date
type
Contribution to journal
publication status
published
subject
in
Molecular & Cellular Proteomics
volume
4
issue
9
pages
1406 - 1408
publisher
American Society for Biochemistry and Molecular Biology
external identifiers
  • pmid:15911533
  • scopus:26844575542
ISSN
1535-9484
DOI
10.1074/mcp.D500003-MCP200
language
English
LU publication?
no
id
22193647-1275-479a-82ed-72af60d04262 (old id 1134231)
date added to LUP
2008-06-18 09:09:21
date last changed
2017-01-01 05:19:45
@article{22193647-1275-479a-82ed-72af60d04262,
  abstract     = {Diseases of the cornea are extremely common and cause severe visual impairment worldwide. To explore the basic molecular mechanisms involved in corneal health and disease, the present study characterizes the proteome of the normal human cornea. All proteins were extracted from the central 7-mm region of 12 normal human donor corneas containing all layers: epithelium, Bowman's layer, stroma, Descemet's membrane, and endothelium. Proteins were fractionated and identified using two different procedures: (i) two-dimensional gel electrophoresis and protein identification by MALDI-MS and (ii) strong cation exchange or one-dimensional SDS gel electrophoresis followed by LC-MS/MS. All together, 141 distinct proteins were identified of which 99 had not previously been identified in any mammalian corneas by direct protein identification methods. The characterized proteins are involved in many processes including antiangiogenesis, antimicrobial defense, protection from and transport of heme and iron, tissue protection against UV radiation and oxidative stress, cell metabolism, and maintenance of intracellular and extracellular structures and stability. This proteome study of the healthy human cornea provides a basis for further analysis of corneal diseases and the design of bioengineered corneas.},
  author       = {Karring, Henrik and Thogersen, Ida B and Klintworth, Gordon K and Moller-Pedersen, Torben and Enghild, Jan J},
  issn         = {1535-9484},
  language     = {eng},
  number       = {9},
  pages        = {1406--1408},
  publisher    = {American Society for Biochemistry and Molecular Biology},
  series       = {Molecular & Cellular Proteomics},
  title        = {A dataset of human cornea proteins identified by Peptide mass fingerprinting and tandem mass spectrometry},
  url          = {http://dx.doi.org/10.1074/mcp.D500003-MCP200},
  volume       = {4},
  year         = {2005},
}