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IGF-I/insulin hybrid receptors in human endothelial cells

Dekker Nitert, Marloes LU ; Chisalita, Simona I; Olsson, Karolina; Bornfeldt, Karin E and Arnqvist, Hans J (2005) In Molecular and Cellular Endocrinology 229(1-2). p.31-37
Abstract
Vascular complications are common in diabetes. IGF-I receptors (IGF-IR) and insulin receptors (IR) in endothelial cells might respond to altered levels of IGF-I and insulin, resulting in altered endothelial function in diabetes. We therefore studied IGF-IR and IR gene expression, ligand binding, receptor protein, and phosphorylation in human umbilical vein endothelial cells (HUVEC). IGF-IR mRNA was more abundant than IR mRNA in freshly isolated HUVEC (IGF-IR/IR ratio 7.1 +/- 1.5) and in cultured HUVEC (ratio 3.5 +/- 0.51). Accordingly, specific binding of (125)I-IGF-I (0.64 +/- 0.25%) was higher than that of (125)I-insulin (0.25 +/- 0.09%). Protein was detected for both receptors and IGF-I/insulin hybrid receptors. IGF-IR phosphorylation... (More)
Vascular complications are common in diabetes. IGF-I receptors (IGF-IR) and insulin receptors (IR) in endothelial cells might respond to altered levels of IGF-I and insulin, resulting in altered endothelial function in diabetes. We therefore studied IGF-IR and IR gene expression, ligand binding, receptor protein, and phosphorylation in human umbilical vein endothelial cells (HUVEC). IGF-IR mRNA was more abundant than IR mRNA in freshly isolated HUVEC (IGF-IR/IR ratio 7.1 +/- 1.5) and in cultured HUVEC (ratio 3.5 +/- 0.51). Accordingly, specific binding of (125)I-IGF-I (0.64 +/- 0.25%) was higher than that of (125)I-insulin (0.25 +/- 0.09%). Protein was detected for both receptors and IGF-I/insulin hybrid receptors. IGF-IR phosphorylation was stimulated by 10(-10) to 10(-8) M IGF-I. IR were activated by 10(-9) to 10(-8) M insulin and IGF-I. We conclude that HUVEC express more IGF-IR than IR, and also express hybrid receptors. Both IGF-I and insulin phosphorylate their own receptors but only IGF-I seems to phosphorylate hybrid receptors. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Insulin/IGF-I hybrid receptor, Endothelial cells, Human, Insulin receptor, IGF-I receptor
in
Molecular and Cellular Endocrinology
volume
229
issue
1-2
pages
31 - 37
publisher
Elsevier
external identifiers
  • pmid:15607526
  • scopus:10944245848
ISSN
1872-8057
DOI
10.1016/j.mce.2004.10.003
language
English
LU publication?
yes
id
77bad997-cac5-42ea-af37-1c0d96b8ebad (old id 1134306)
date added to LUP
2008-06-16 16:14:13
date last changed
2017-07-23 03:54:48
@article{77bad997-cac5-42ea-af37-1c0d96b8ebad,
  abstract     = {Vascular complications are common in diabetes. IGF-I receptors (IGF-IR) and insulin receptors (IR) in endothelial cells might respond to altered levels of IGF-I and insulin, resulting in altered endothelial function in diabetes. We therefore studied IGF-IR and IR gene expression, ligand binding, receptor protein, and phosphorylation in human umbilical vein endothelial cells (HUVEC). IGF-IR mRNA was more abundant than IR mRNA in freshly isolated HUVEC (IGF-IR/IR ratio 7.1 +/- 1.5) and in cultured HUVEC (ratio 3.5 +/- 0.51). Accordingly, specific binding of (125)I-IGF-I (0.64 +/- 0.25%) was higher than that of (125)I-insulin (0.25 +/- 0.09%). Protein was detected for both receptors and IGF-I/insulin hybrid receptors. IGF-IR phosphorylation was stimulated by 10(-10) to 10(-8) M IGF-I. IR were activated by 10(-9) to 10(-8) M insulin and IGF-I. We conclude that HUVEC express more IGF-IR than IR, and also express hybrid receptors. Both IGF-I and insulin phosphorylate their own receptors but only IGF-I seems to phosphorylate hybrid receptors.},
  author       = {Dekker Nitert, Marloes and Chisalita, Simona I and Olsson, Karolina and Bornfeldt, Karin E and Arnqvist, Hans J},
  issn         = {1872-8057},
  keyword      = {Insulin/IGF-I hybrid receptor,Endothelial cells,Human,Insulin receptor,IGF-I receptor},
  language     = {eng},
  number       = {1-2},
  pages        = {31--37},
  publisher    = {Elsevier},
  series       = {Molecular and Cellular Endocrinology},
  title        = {IGF-I/insulin hybrid receptors in human endothelial cells},
  url          = {http://dx.doi.org/10.1016/j.mce.2004.10.003},
  volume       = {229},
  year         = {2005},
}