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Capacity of human beta-defensin expression in gene-transduced and cytokine-induced cells

Yin, Chunyi ; Dang, Hoa N ; Zhang, Hai-Bo ; Gazor, Farzad ; Kim, Daniel ; Sørensen, Ole E LU and Huang, George T-J (2006) In Biochemical and Biophysical Research Communications 339(1). p.344-354
Abstract
The purpose of this study was to determine the capacity of cells transduced with human beta-defensins (HBDs) to express antimicrobial peptides, since sufficient expression level is required for effective antimicrobial activity. Retroviral vector pBabeNeo and lentiviral vector SIN18cPPTRhMLV (SIN18) carrying HBDs were utilized to transduce non-HBD-expressing cells such as fibroblasts or HBD-producing oral epithelial cells. We found that HBD-3 gene transfer to fibroblasts was possible not via retrovirus but by direct vector transfection. SIN18 had high transduction efficiencies (80.9-99.9%) and transduced cells expressed higher amounts of HBD-2 than those by pBabeNeo. Primary human gingival epithelial cells (HGECs) expressed greater amounts... (More)
The purpose of this study was to determine the capacity of cells transduced with human beta-defensins (HBDs) to express antimicrobial peptides, since sufficient expression level is required for effective antimicrobial activity. Retroviral vector pBabeNeo and lentiviral vector SIN18cPPTRhMLV (SIN18) carrying HBDs were utilized to transduce non-HBD-expressing cells such as fibroblasts or HBD-producing oral epithelial cells. We found that HBD-3 gene transfer to fibroblasts was possible not via retrovirus but by direct vector transfection. SIN18 had high transduction efficiencies (80.9-99.9%) and transduced cells expressed higher amounts of HBD-2 than those by pBabeNeo. Primary human gingival epithelial cells (HGECs) expressed greater amounts of HBD-2 than primary fibroblasts after lentiviral transduction. Additionally, HBD-2 secretion from transduced HGECs cells was further increased when stimulated with IL-1 or TNFalpha. Our data indicate that while HBD-2 expression is limited in primary fibroblasts, its expression in HGECs may be maximized by gene transduction plus cytokine induction. (Less)
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author
; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
HBD-2, HBD-3, Lentiviral vectors, Retroviral vectors, IL-1, TNFgreek small letter alpha, Gingival epithelial cells
in
Biochemical and Biophysical Research Communications
volume
339
issue
1
pages
344 - 354
publisher
Elsevier
external identifiers
  • pmid:16298338
  • scopus:28144446129
ISSN
1090-2104
DOI
10.1016/j.bbrc.2005.11.020
language
English
LU publication?
yes
id
5c12a09c-5d5c-4653-bfcf-dece384204a6 (old id 1135255)
date added to LUP
2016-04-01 16:03:57
date last changed
2020-01-12 18:59:55
@article{5c12a09c-5d5c-4653-bfcf-dece384204a6,
  abstract     = {The purpose of this study was to determine the capacity of cells transduced with human beta-defensins (HBDs) to express antimicrobial peptides, since sufficient expression level is required for effective antimicrobial activity. Retroviral vector pBabeNeo and lentiviral vector SIN18cPPTRhMLV (SIN18) carrying HBDs were utilized to transduce non-HBD-expressing cells such as fibroblasts or HBD-producing oral epithelial cells. We found that HBD-3 gene transfer to fibroblasts was possible not via retrovirus but by direct vector transfection. SIN18 had high transduction efficiencies (80.9-99.9%) and transduced cells expressed higher amounts of HBD-2 than those by pBabeNeo. Primary human gingival epithelial cells (HGECs) expressed greater amounts of HBD-2 than primary fibroblasts after lentiviral transduction. Additionally, HBD-2 secretion from transduced HGECs cells was further increased when stimulated with IL-1 or TNFalpha. Our data indicate that while HBD-2 expression is limited in primary fibroblasts, its expression in HGECs may be maximized by gene transduction plus cytokine induction.},
  author       = {Yin, Chunyi and Dang, Hoa N and Zhang, Hai-Bo and Gazor, Farzad and Kim, Daniel and Sørensen, Ole E and Huang, George T-J},
  issn         = {1090-2104},
  language     = {eng},
  number       = {1},
  pages        = {344--354},
  publisher    = {Elsevier},
  series       = {Biochemical and Biophysical Research Communications},
  title        = {Capacity of human beta-defensin expression in gene-transduced and cytokine-induced cells},
  url          = {http://dx.doi.org/10.1016/j.bbrc.2005.11.020},
  doi          = {10.1016/j.bbrc.2005.11.020},
  volume       = {339},
  year         = {2006},
}