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In vitro activity of synthetic antimicrobial peptides against Candida

Kamysz, Wojciech ; Nadolski, Piotr ; Kedzia, Anna ; Cirioni, Oscar ; Barchiesi, Francesco ; Giacometti, Andrea ; Scalise, Giorgio ; Lukasiak, Jerzy and Okroj, Marcin LU (2006) In Polish Journal of Microbiology 55(4). p.303-307
Abstract
Yeast-like fungi are the most common cause of fungal infections in humans. Actually, in the age of opportunistic infections and increasing resistance, development of modern antifungal agents becomes a very important challenge. This paper describes synthesis and antimicrobial assay of four naturally occurring peptide antibiotics (aurein 1.2, citropin 1.1, temporin A, uperin 3.6) and three chemically engineered analogues actually passing clinical trials (iseganan, pexiganan, omiganan) against Candida strains isolated from patients with infections of the oral cavity or respiratory tract. The peptides were synthesized using solid-phase method and purified by high-performance liquid chromatography. Biological tests were performed using the... (More)
Yeast-like fungi are the most common cause of fungal infections in humans. Actually, in the age of opportunistic infections and increasing resistance, development of modern antifungal agents becomes a very important challenge. This paper describes synthesis and antimicrobial assay of four naturally occurring peptide antibiotics (aurein 1.2, citropin 1.1, temporin A, uperin 3.6) and three chemically engineered analogues actually passing clinical trials (iseganan, pexiganan, omiganan) against Candida strains isolated from patients with infections of the oral cavity or respiratory tract. The peptides were synthesized using solid-phase method and purified by high-performance liquid chromatography. Biological tests were performed using the broth microdilution method. The antifungal activity of the peptide antibiotics was compared to that of nystatin and amphotericin B. We found synthetic peptides to be generally less potent than amphotericin B or nystatin. However, some of the naturally occurring peptides still retained reasonable antifungal activities which were higher than these of iseganan, pexiganan or omiganan. We think that the naturally occurring peptide antibiotics included in our study can be a good matrix for development of novel antifungal compounds. (Less)
Please use this url to cite or link to this publication:
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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Polish Journal of Microbiology
volume
55
issue
4
pages
303 - 307
publisher
Polskie Towarzystwo Mikrobiologów
external identifiers
  • pmid:17416067
  • scopus:33846159109
ISSN
1733-1331
language
English
LU publication?
yes
id
e4321e08-924a-4acd-8db0-466380153bf9 (old id 1135381)
alternative location
http://www.amp.microbiology.pl/full/vol5542006f.pdf#page=57
date added to LUP
2016-04-01 15:52:30
date last changed
2021-10-06 05:47:49
@article{e4321e08-924a-4acd-8db0-466380153bf9,
  abstract     = {Yeast-like fungi are the most common cause of fungal infections in humans. Actually, in the age of opportunistic infections and increasing resistance, development of modern antifungal agents becomes a very important challenge. This paper describes synthesis and antimicrobial assay of four naturally occurring peptide antibiotics (aurein 1.2, citropin 1.1, temporin A, uperin 3.6) and three chemically engineered analogues actually passing clinical trials (iseganan, pexiganan, omiganan) against Candida strains isolated from patients with infections of the oral cavity or respiratory tract. The peptides were synthesized using solid-phase method and purified by high-performance liquid chromatography. Biological tests were performed using the broth microdilution method. The antifungal activity of the peptide antibiotics was compared to that of nystatin and amphotericin B. We found synthetic peptides to be generally less potent than amphotericin B or nystatin. However, some of the naturally occurring peptides still retained reasonable antifungal activities which were higher than these of iseganan, pexiganan or omiganan. We think that the naturally occurring peptide antibiotics included in our study can be a good matrix for development of novel antifungal compounds.},
  author       = {Kamysz, Wojciech and Nadolski, Piotr and Kedzia, Anna and Cirioni, Oscar and Barchiesi, Francesco and Giacometti, Andrea and Scalise, Giorgio and Lukasiak, Jerzy and Okroj, Marcin},
  issn         = {1733-1331},
  language     = {eng},
  number       = {4},
  pages        = {303--307},
  publisher    = {Polskie Towarzystwo Mikrobiologów},
  series       = {Polish Journal of Microbiology},
  title        = {In vitro activity of synthetic antimicrobial peptides against Candida},
  url          = {http://www.amp.microbiology.pl/full/vol5542006f.pdf#page=57},
  volume       = {55},
  year         = {2006},
}