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Nitric oxide radicals in leucocytes and vaginal washes of Trichomonas vaginalis -infected symptomatic and asymptomatic women

Yadav, Manisha LU ; Dubey, M L ; Gupta, I and Malla, N (2006) In Parasitology 132(Pt 3). p.339-343
Abstract
The clinical spectrum of Trichomonas vaginalis infection varies from asymptomatic to mild, moderate or severe vaginitis. Nitric oxide and other reactive nitrogen radicals produced by immune effector cells are important cytotoxic and cytostatic mediators against several microorganisms including parasites. In the present study, inducible nitric oxide synthase (iNOS) and reactive nitrogen intermediates (RNI) were determined in leucocyte cultures (stimulated with T. vaginalis in vitro) and vaginal washes (VWs) of 22 symptomatic and 20 asymptomatic T. vaginalis-infected and 20 healthy women by immunoblotting and Griess method respectively. The iNOS protein was detected in leucocytes and VWs of all the symptomatic and asymptomatic women, but was... (More)
The clinical spectrum of Trichomonas vaginalis infection varies from asymptomatic to mild, moderate or severe vaginitis. Nitric oxide and other reactive nitrogen radicals produced by immune effector cells are important cytotoxic and cytostatic mediators against several microorganisms including parasites. In the present study, inducible nitric oxide synthase (iNOS) and reactive nitrogen intermediates (RNI) were determined in leucocyte cultures (stimulated with T. vaginalis in vitro) and vaginal washes (VWs) of 22 symptomatic and 20 asymptomatic T. vaginalis-infected and 20 healthy women by immunoblotting and Griess method respectively. The iNOS protein was detected in leucocytes and VWs of all the symptomatic and asymptomatic women, but was not detected in any of the samples from healthy women. Mean iNOS protein band intensity was significantly higher in leucocytes as compared to VWs (P<0.001) of both symptomatic and asymptomatic women and was also higher in leucocytes of asymptomatic as compared to symptomatic women (P<0.05). Mean RNI concentration was also significantly higher in leucocytes (P<0.01) and VWs (P<0.05) of asymptomatic as compared to symptomatic women, and was also higher in samples of infected as compared to healthy women (P<0.001). These results suggest that reactive nitrogen radicals may have a role in limiting T. vaginalis infection in asymptomatic women. (Less)
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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
inducible nitric oxide synthase, reactive nitrogen intermediates, Trichomonas vaginalis, trichomoniasis
in
Parasitology
volume
132
issue
Pt 3
pages
339 - 343
publisher
Cambridge University Press
external identifiers
  • pmid:16529664
  • scopus:33644872912
ISSN
1469-8161
DOI
10.1017/S0031182005009340
language
English
LU publication?
yes
id
c8d42f0e-5add-4686-a1f5-273c8eb6bf73 (old id 1137335)
date added to LUP
2016-04-01 12:00:56
date last changed
2021-09-29 03:44:48
@article{c8d42f0e-5add-4686-a1f5-273c8eb6bf73,
  abstract     = {The clinical spectrum of Trichomonas vaginalis infection varies from asymptomatic to mild, moderate or severe vaginitis. Nitric oxide and other reactive nitrogen radicals produced by immune effector cells are important cytotoxic and cytostatic mediators against several microorganisms including parasites. In the present study, inducible nitric oxide synthase (iNOS) and reactive nitrogen intermediates (RNI) were determined in leucocyte cultures (stimulated with T. vaginalis in vitro) and vaginal washes (VWs) of 22 symptomatic and 20 asymptomatic T. vaginalis-infected and 20 healthy women by immunoblotting and Griess method respectively. The iNOS protein was detected in leucocytes and VWs of all the symptomatic and asymptomatic women, but was not detected in any of the samples from healthy women. Mean iNOS protein band intensity was significantly higher in leucocytes as compared to VWs (P&lt;0.001) of both symptomatic and asymptomatic women and was also higher in leucocytes of asymptomatic as compared to symptomatic women (P&lt;0.05). Mean RNI concentration was also significantly higher in leucocytes (P&lt;0.01) and VWs (P&lt;0.05) of asymptomatic as compared to symptomatic women, and was also higher in samples of infected as compared to healthy women (P&lt;0.001). These results suggest that reactive nitrogen radicals may have a role in limiting T. vaginalis infection in asymptomatic women.},
  author       = {Yadav, Manisha and Dubey, M L and Gupta, I and Malla, N},
  issn         = {1469-8161},
  language     = {eng},
  number       = {Pt 3},
  pages        = {339--343},
  publisher    = {Cambridge University Press},
  series       = {Parasitology},
  title        = {Nitric oxide radicals in leucocytes and vaginal washes of Trichomonas vaginalis -infected symptomatic and asymptomatic women},
  url          = {http://dx.doi.org/10.1017/S0031182005009340},
  doi          = {10.1017/S0031182005009340},
  volume       = {132},
  year         = {2006},
}