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Sperm chromatin structure assay parameters measured after density gradient centrifugation are not predictive for the outcome of ART

Bungum, Mona LU ; Spano, Marcello; Humaidan, Peter; Eleuteri, Patrizia; Rescia, Michele and Giwercman, Aleksander LU (2008) In Human Reproduction 23(1). p.41374-41374
Abstract
BACKGROUND The sperm chromatin structure assay (SCSA) parameter DNA fragmentation index (DFI) has been shown to predict in vivo and in vitro fertility. So far most SCSA studies have been based on SCSA analysis performed on neat semen. The aim of this study is to assess whether SCSA analysis of sperm prepared by density gradient centrifugation (DGC) could add more information in regard to the prediction of treatment outcome. METHODS The study included 510 assisted reproductive technique (ART) cycles. SCSA was performed in neat semen and post DGC. SCSA results were expressed in terms of DFI and high DNA stainability (HDS) cell fractions. The outcome parameter was clinical pregnancy (CP). RESULTS Scatter-plot diagrams demonstrated that for... (More)
BACKGROUND The sperm chromatin structure assay (SCSA) parameter DNA fragmentation index (DFI) has been shown to predict in vivo and in vitro fertility. So far most SCSA studies have been based on SCSA analysis performed on neat semen. The aim of this study is to assess whether SCSA analysis of sperm prepared by density gradient centrifugation (DGC) could add more information in regard to the prediction of treatment outcome. METHODS The study included 510 assisted reproductive technique (ART) cycles. SCSA was performed in neat semen and post DGC. SCSA results were expressed in terms of DFI and high DNA stainability (HDS) cell fractions. The outcome parameter was clinical pregnancy (CP). RESULTS Scatter-plot diagrams demonstrated that for DGC samples, no DFI cut-off values could be set for in vivo or in vitro fertility. In intrauterine insemination, IVF and ICSI groups the mean difference (95% CI) in DFI post DGC between those who achieved CP and those who did not was 0.2% (-1.7 to 2.0%), 0.4% (-1.9 to 2.8%) and 1.3% (-3.1 to 5.9%), respectively, none of these being statistically significant. The corresponding differences for HDS were 0.1% (-1.3 to 1.5%), 0.1% (-0.7 to 0.9%) and 0.6% (-1.6 to 2.7%), respectively (all P-values >0.6). CONCLUSIONS SCSA performed in semen prepared by DGC cannot predict the outcome of ART. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Human Reproduction
volume
23
issue
1
pages
41374 - 41374
publisher
Oxford University Press
external identifiers
  • pmid:17986484
  • wos:000252847300002
  • scopus:40549105428
ISSN
0268-1161
DOI
10.1093/humrep/dem353
language
English
LU publication?
yes
id
2957e3fb-eca9-4e99-905e-a03d0d1c8800 (old id 1137961)
date added to LUP
2008-08-05 14:31:57
date last changed
2017-09-10 03:47:25
@article{2957e3fb-eca9-4e99-905e-a03d0d1c8800,
  abstract     = {BACKGROUND The sperm chromatin structure assay (SCSA) parameter DNA fragmentation index (DFI) has been shown to predict in vivo and in vitro fertility. So far most SCSA studies have been based on SCSA analysis performed on neat semen. The aim of this study is to assess whether SCSA analysis of sperm prepared by density gradient centrifugation (DGC) could add more information in regard to the prediction of treatment outcome. METHODS The study included 510 assisted reproductive technique (ART) cycles. SCSA was performed in neat semen and post DGC. SCSA results were expressed in terms of DFI and high DNA stainability (HDS) cell fractions. The outcome parameter was clinical pregnancy (CP). RESULTS Scatter-plot diagrams demonstrated that for DGC samples, no DFI cut-off values could be set for in vivo or in vitro fertility. In intrauterine insemination, IVF and ICSI groups the mean difference (95% CI) in DFI post DGC between those who achieved CP and those who did not was 0.2% (-1.7 to 2.0%), 0.4% (-1.9 to 2.8%) and 1.3% (-3.1 to 5.9%), respectively, none of these being statistically significant. The corresponding differences for HDS were 0.1% (-1.3 to 1.5%), 0.1% (-0.7 to 0.9%) and 0.6% (-1.6 to 2.7%), respectively (all P-values >0.6). CONCLUSIONS SCSA performed in semen prepared by DGC cannot predict the outcome of ART.},
  author       = {Bungum, Mona and Spano, Marcello and Humaidan, Peter and Eleuteri, Patrizia and Rescia, Michele and Giwercman, Aleksander},
  issn         = {0268-1161},
  language     = {eng},
  number       = {1},
  pages        = {41374--41374},
  publisher    = {Oxford University Press},
  series       = {Human Reproduction},
  title        = {Sperm chromatin structure assay parameters measured after density gradient centrifugation are not predictive for the outcome of ART},
  url          = {http://dx.doi.org/10.1093/humrep/dem353},
  volume       = {23},
  year         = {2008},
}