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The Cotton-Top Tamarin (Saguinus oedipus) Has Five beta-Microseminoprotein Genes, Two of Which Are Pseudogenes

Valtonen-André, Camilla LU and Lundwall, Åke LU (2008) In DNA and Cell Biology 27(1). p.45-54
Abstract
beta-Microseminoprotein (MSP) is one of the most abundant proteins in human seminal plasma and is secreted from the prostate gland. Its evolution can be traced from primates down to nonvertebrate species such as amphioxus, despite substantial differences in the primary structure. Most mammals are known to have one single MSP gene, but we have previously shown that the cotton-top tamarin and the common marmoset-two New World monkeys-carry several MSP genes. In this study we continue our characterization of MSP genes in the cotton-top tamarin by presenting the full nucleotide sequence of the three previously identified genes, mspA, mspE, and mspJ. A promoter analysis using luciferase reporter showed that mspE is as transcriptionally active... (More)
beta-Microseminoprotein (MSP) is one of the most abundant proteins in human seminal plasma and is secreted from the prostate gland. Its evolution can be traced from primates down to nonvertebrate species such as amphioxus, despite substantial differences in the primary structure. Most mammals are known to have one single MSP gene, but we have previously shown that the cotton-top tamarin and the common marmoset-two New World monkeys-carry several MSP genes. In this study we continue our characterization of MSP genes in the cotton-top tamarin by presenting the full nucleotide sequence of the three previously identified genes, mspA, mspE, and mspJ. A promoter analysis using luciferase reporter showed that mspE is as transcriptionally active as the single human MSP gene, whereas mspA and mspJ display no activity with this assay. Two novel MSP genes were also identified, mspB and mspH, both of which are pseudogenes. MspB has a frameshift mutation in the third exon resulting in a new C-terminus and premature stop of translation. MspH has the features of a processed pseudogene, originating from a transcript of mspE. It is integrated into the genome together with another processed pseudogene originating from a transcript of the nucleoporin gene NUP88. The MSP genes described in this study probably arose by phylogenetically rather late duplication or retrotransposition, suggesting that they are confined to a limited number of New World monkeys. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
DNA and Cell Biology
volume
27
issue
1
pages
45 - 54
publisher
Mary Ann Liebert, Inc.
external identifiers
  • pmid:18020964
  • wos:000252559500006
  • scopus:38349107900
ISSN
1044-5498
DOI
10.1089/dna.2007.0641
language
English
LU publication?
yes
id
824075e9-3429-477c-a951-2dc36e397968 (old id 1138710)
date added to LUP
2008-08-05 13:23:38
date last changed
2017-01-01 05:49:05
@article{824075e9-3429-477c-a951-2dc36e397968,
  abstract     = {beta-Microseminoprotein (MSP) is one of the most abundant proteins in human seminal plasma and is secreted from the prostate gland. Its evolution can be traced from primates down to nonvertebrate species such as amphioxus, despite substantial differences in the primary structure. Most mammals are known to have one single MSP gene, but we have previously shown that the cotton-top tamarin and the common marmoset-two New World monkeys-carry several MSP genes. In this study we continue our characterization of MSP genes in the cotton-top tamarin by presenting the full nucleotide sequence of the three previously identified genes, mspA, mspE, and mspJ. A promoter analysis using luciferase reporter showed that mspE is as transcriptionally active as the single human MSP gene, whereas mspA and mspJ display no activity with this assay. Two novel MSP genes were also identified, mspB and mspH, both of which are pseudogenes. MspB has a frameshift mutation in the third exon resulting in a new C-terminus and premature stop of translation. MspH has the features of a processed pseudogene, originating from a transcript of mspE. It is integrated into the genome together with another processed pseudogene originating from a transcript of the nucleoporin gene NUP88. The MSP genes described in this study probably arose by phylogenetically rather late duplication or retrotransposition, suggesting that they are confined to a limited number of New World monkeys.},
  author       = {Valtonen-André, Camilla and Lundwall, Åke},
  issn         = {1044-5498},
  language     = {eng},
  number       = {1},
  pages        = {45--54},
  publisher    = {Mary Ann Liebert, Inc.},
  series       = {DNA and Cell Biology},
  title        = {The Cotton-Top Tamarin (Saguinus oedipus) Has Five beta-Microseminoprotein Genes, Two of Which Are Pseudogenes},
  url          = {http://dx.doi.org/10.1089/dna.2007.0641},
  volume       = {27},
  year         = {2008},
}