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Modifying the red cell surface: towards an ABO-universal blood supply

Olsson, Martin L LU and Clausen, Henrik (2008) In British Journal of Haematology 140(1). p.3-12
Abstract
Eliminating the risk for ABO-incompatible transfusion errors and simplifying logistics by creating a universal blood inventory is a challenging idea. Goldstein and co-workers pioneered the field of enzymatic conversion of blood group A and B red blood cells (RBCs) to O (ECO). Using alpha-galactosidase from coffee beans to produce B-ECO RBCs, proof of principle for this revolutionary concept was achieved in clinical trials. However, because this enzyme has poor kinetic properties and low pH optimum the process was not economically viable. Conversion of group A RBCs was only achieved with the weak A(2) subgroup with related enzymes having acidic pH optima. More recently, the identification of entirely new families of bacterial... (More)
Eliminating the risk for ABO-incompatible transfusion errors and simplifying logistics by creating a universal blood inventory is a challenging idea. Goldstein and co-workers pioneered the field of enzymatic conversion of blood group A and B red blood cells (RBCs) to O (ECO). Using alpha-galactosidase from coffee beans to produce B-ECO RBCs, proof of principle for this revolutionary concept was achieved in clinical trials. However, because this enzyme has poor kinetic properties and low pH optimum the process was not economically viable. Conversion of group A RBCs was only achieved with the weak A(2) subgroup with related enzymes having acidic pH optima. More recently, the identification of entirely new families of bacterial exoglycosidases with remarkably improved kinetic properties for cleaving A and B antigens has reinvigorated the field. Enzymatic conversion of groups A, B and AB RBCs with these novel enzymes resulting in ECO RBCs typing as O can now be achieved with low enzyme protein consumption, short incubation times and at neutral pH. Presently, clinical trials evaluating safety and efficacy of ECO RBCs are ongoing. Here, we review the status of the ECO technology, its impact and potential for introduction into clinical component preparation laboratories. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
glycosidase, ABO blood group, transfusion, carbohydrate antigens, red cell
in
British Journal of Haematology
volume
140
issue
1
pages
3 - 12
publisher
Federation of European Neuroscience Societies and Blackwell Publishing Ltd
external identifiers
  • pmid:17970801
  • wos:000251502700001
  • scopus:36849021005
ISSN
0007-1048
DOI
10.1111/j.1365-2141.2007.06839.x
language
English
LU publication?
yes
id
a473fa92-645c-47c9-8f17-fa071d2bea60 (old id 1138823)
date added to LUP
2008-08-20 08:33:22
date last changed
2017-07-30 03:33:33
@article{a473fa92-645c-47c9-8f17-fa071d2bea60,
  abstract     = {Eliminating the risk for ABO-incompatible transfusion errors and simplifying logistics by creating a universal blood inventory is a challenging idea. Goldstein and co-workers pioneered the field of enzymatic conversion of blood group A and B red blood cells (RBCs) to O (ECO). Using alpha-galactosidase from coffee beans to produce B-ECO RBCs, proof of principle for this revolutionary concept was achieved in clinical trials. However, because this enzyme has poor kinetic properties and low pH optimum the process was not economically viable. Conversion of group A RBCs was only achieved with the weak A(2) subgroup with related enzymes having acidic pH optima. More recently, the identification of entirely new families of bacterial exoglycosidases with remarkably improved kinetic properties for cleaving A and B antigens has reinvigorated the field. Enzymatic conversion of groups A, B and AB RBCs with these novel enzymes resulting in ECO RBCs typing as O can now be achieved with low enzyme protein consumption, short incubation times and at neutral pH. Presently, clinical trials evaluating safety and efficacy of ECO RBCs are ongoing. Here, we review the status of the ECO technology, its impact and potential for introduction into clinical component preparation laboratories.},
  author       = {Olsson, Martin L and Clausen, Henrik},
  issn         = {0007-1048},
  keyword      = {glycosidase,ABO blood group,transfusion,carbohydrate antigens,red cell},
  language     = {eng},
  number       = {1},
  pages        = {3--12},
  publisher    = {Federation of European Neuroscience Societies and Blackwell Publishing Ltd},
  series       = {British Journal of Haematology},
  title        = {Modifying the red cell surface: towards an ABO-universal blood supply},
  url          = {http://dx.doi.org/10.1111/j.1365-2141.2007.06839.x},
  volume       = {140},
  year         = {2008},
}