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SPI-C, a PU-box binding ETS protein expressed temporarily during B-cell development and in macrophages, contains an acidic transactivation domain located to the N-terminus.

Carlsson, Robert LU ; Persson, Christine LU and Leanderson, Tomas LU (2003) In Molecular Immunology 39(16). p.1035-1043
Abstract
Mice deficient for SPI-group ETS transcription factors PU.1 or SPI-B fail to generate lymphocytes or do not mount normal antibody mediated immune responses, respectively. PU.1 expression is restricted to B-, T-lymphocytes and macrophages, while SPI-B is expressed in B- and T-lymphocytes. SPI-C is an ETS transcription factor closely related to PU.1 and SPI-B, and expressed temporarily during B-cell development and in macrophages. By deletion and mutation analysis we show that the SPI-C protein has a transactivation domain located to the N-terminus, and that the transactivation activity is reduced to that of the DNA binding domain (DBD) alone when four aspartic acid residues are mutated to alanines. PU.1 and SPI-B regulate transcription from... (More)
Mice deficient for SPI-group ETS transcription factors PU.1 or SPI-B fail to generate lymphocytes or do not mount normal antibody mediated immune responses, respectively. PU.1 expression is restricted to B-, T-lymphocytes and macrophages, while SPI-B is expressed in B- and T-lymphocytes. SPI-C is an ETS transcription factor closely related to PU.1 and SPI-B, and expressed temporarily during B-cell development and in macrophages. By deletion and mutation analysis we show that the SPI-C protein has a transactivation domain located to the N-terminus, and that the transactivation activity is reduced to that of the DNA binding domain (DBD) alone when four aspartic acid residues are mutated to alanines. PU.1 and SPI-B regulate transcription from acidic domains located to the N-terminus and by recruiting the co-activator PIP to adjacent sites in a sequence specific manner. In contrast to PU.1 and PIP, SPI-C and PIP were unable to form a distinct ternary complex on the Ig λ light chain λ2–4 enhancer element, suggesting that SPI-C could act both as a positive and negative transcriptional regulator during B-lymphocyte differentiation. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Transcription, PU.1, His-tag, Modular, Substitution
in
Molecular Immunology
volume
39
issue
16
pages
1035 - 1043
publisher
Pergamon
external identifiers
  • wos:000183119800006
  • pmid:12749910
  • scopus:0038056175
ISSN
1872-9142
DOI
language
English
LU publication?
yes
id
3f99614b-27cb-41bf-867f-12b77d4a7415 (old id 114013)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12749910&dopt=Abstract
date added to LUP
2007-07-11 09:50:24
date last changed
2018-05-29 11:46:18
@article{3f99614b-27cb-41bf-867f-12b77d4a7415,
  abstract     = {Mice deficient for SPI-group ETS transcription factors PU.1 or SPI-B fail to generate lymphocytes or do not mount normal antibody mediated immune responses, respectively. PU.1 expression is restricted to B-, T-lymphocytes and macrophages, while SPI-B is expressed in B- and T-lymphocytes. SPI-C is an ETS transcription factor closely related to PU.1 and SPI-B, and expressed temporarily during B-cell development and in macrophages. By deletion and mutation analysis we show that the SPI-C protein has a transactivation domain located to the N-terminus, and that the transactivation activity is reduced to that of the DNA binding domain (DBD) alone when four aspartic acid residues are mutated to alanines. PU.1 and SPI-B regulate transcription from acidic domains located to the N-terminus and by recruiting the co-activator PIP to adjacent sites in a sequence specific manner. In contrast to PU.1 and PIP, SPI-C and PIP were unable to form a distinct ternary complex on the Ig λ light chain λ2–4 enhancer element, suggesting that SPI-C could act both as a positive and negative transcriptional regulator during B-lymphocyte differentiation.},
  author       = {Carlsson, Robert and Persson, Christine and Leanderson, Tomas},
  issn         = {1872-9142},
  keyword      = {Transcription,PU.1,His-tag,Modular,Substitution},
  language     = {eng},
  number       = {16},
  pages        = {1035--1043},
  publisher    = {Pergamon},
  series       = {Molecular Immunology},
  title        = {SPI-C, a PU-box binding ETS protein expressed temporarily during B-cell development and in macrophages, contains an acidic transactivation domain located to the N-terminus.},
  url          = {http://dx.doi.org/},
  volume       = {39},
  year         = {2003},
}