Reduced expression of CYLD in human colon and hepatocellular carcinomas
(2007) In Carcinogenesis 28(1). p.21-27- Abstract
- CYLD was originally identified as a tumor suppressor that is mutated in familial cylindromatosis. Recent studies suggested a role for CYLD in nuclear factor-kappaB (NF-kappaB) regulation. NF-kappaB activation has been connected with multiple aspects of oncogenesis but the underlying molecular mechanisms of persistent NF-kappaB activation in tumors remain largely unknown. Thus, we evaluated CYLD transcription in different colon and hepatocellular carcinoma cell lines and tissue samples, respectively. CYLD was downregulated or lost in all tumor cell lines investigated as compared with primary human colonic epithelial cells and hepatocytes, respectively. Further, quantitative PCR analysis revealed reduced CYLD mRNA expression in most tumor... (More)
- CYLD was originally identified as a tumor suppressor that is mutated in familial cylindromatosis. Recent studies suggested a role for CYLD in nuclear factor-kappaB (NF-kappaB) regulation. NF-kappaB activation has been connected with multiple aspects of oncogenesis but the underlying molecular mechanisms of persistent NF-kappaB activation in tumors remain largely unknown. Thus, we evaluated CYLD transcription in different colon and hepatocellular carcinoma cell lines and tissue samples, respectively. CYLD was downregulated or lost in all tumor cell lines investigated as compared with primary human colonic epithelial cells and hepatocytes, respectively. Further, quantitative PCR analysis revealed reduced CYLD mRNA expression in most tumor samples compared with non-tumorous tissue. Analysis on protein level confirmed these findings. Functional assays with CYLD transfected cell lines revealed that CYLD expression decreased NF-kappaB activity. Thus, functional relevant loss of CYLD expression may contribute to tumor development and progression, and may provide a new target for therapeutic strategies. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1141278
- author
- Hellerbrand, Claus ; Bumes, Elisabeth ; Bataille, Frauke ; Dietmaier, Wolfgang ; Massoumi, Ramin LU and Bosserhoff, Anja K
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Carcinogenesis
- volume
- 28
- issue
- 1
- pages
- 21 - 27
- publisher
- Oxford University Press
- external identifiers
-
- pmid:16774947
- scopus:33845679104
- pmid:16774947
- ISSN
- 0143-3334
- DOI
- 10.1093/carcin/bgl081
- language
- English
- LU publication?
- yes
- id
- a7db0c0a-c5d7-46a8-accf-944206381223 (old id 1141278)
- date added to LUP
- 2016-04-04 07:40:39
- date last changed
- 2022-05-09 00:48:51
@article{a7db0c0a-c5d7-46a8-accf-944206381223, abstract = {{CYLD was originally identified as a tumor suppressor that is mutated in familial cylindromatosis. Recent studies suggested a role for CYLD in nuclear factor-kappaB (NF-kappaB) regulation. NF-kappaB activation has been connected with multiple aspects of oncogenesis but the underlying molecular mechanisms of persistent NF-kappaB activation in tumors remain largely unknown. Thus, we evaluated CYLD transcription in different colon and hepatocellular carcinoma cell lines and tissue samples, respectively. CYLD was downregulated or lost in all tumor cell lines investigated as compared with primary human colonic epithelial cells and hepatocytes, respectively. Further, quantitative PCR analysis revealed reduced CYLD mRNA expression in most tumor samples compared with non-tumorous tissue. Analysis on protein level confirmed these findings. Functional assays with CYLD transfected cell lines revealed that CYLD expression decreased NF-kappaB activity. Thus, functional relevant loss of CYLD expression may contribute to tumor development and progression, and may provide a new target for therapeutic strategies.}}, author = {{Hellerbrand, Claus and Bumes, Elisabeth and Bataille, Frauke and Dietmaier, Wolfgang and Massoumi, Ramin and Bosserhoff, Anja K}}, issn = {{0143-3334}}, language = {{eng}}, number = {{1}}, pages = {{21--27}}, publisher = {{Oxford University Press}}, series = {{Carcinogenesis}}, title = {{Reduced expression of CYLD in human colon and hepatocellular carcinomas}}, url = {{http://dx.doi.org/10.1093/carcin/bgl081}}, doi = {{10.1093/carcin/bgl081}}, volume = {{28}}, year = {{2007}}, }