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Reduced expression of CYLD in human colon and hepatocellular carcinomas

Hellerbrand, Claus; Bumes, Elisabeth; Bataille, Frauke; Dietmaier, Wolfgang; Massoumi, Ramin LU and Bosserhoff, Anja K (2007) In Carcinogenesis 28(1). p.21-27
Abstract
CYLD was originally identified as a tumor suppressor that is mutated in familial cylindromatosis. Recent studies suggested a role for CYLD in nuclear factor-kappaB (NF-kappaB) regulation. NF-kappaB activation has been connected with multiple aspects of oncogenesis but the underlying molecular mechanisms of persistent NF-kappaB activation in tumors remain largely unknown. Thus, we evaluated CYLD transcription in different colon and hepatocellular carcinoma cell lines and tissue samples, respectively. CYLD was downregulated or lost in all tumor cell lines investigated as compared with primary human colonic epithelial cells and hepatocytes, respectively. Further, quantitative PCR analysis revealed reduced CYLD mRNA expression in most tumor... (More)
CYLD was originally identified as a tumor suppressor that is mutated in familial cylindromatosis. Recent studies suggested a role for CYLD in nuclear factor-kappaB (NF-kappaB) regulation. NF-kappaB activation has been connected with multiple aspects of oncogenesis but the underlying molecular mechanisms of persistent NF-kappaB activation in tumors remain largely unknown. Thus, we evaluated CYLD transcription in different colon and hepatocellular carcinoma cell lines and tissue samples, respectively. CYLD was downregulated or lost in all tumor cell lines investigated as compared with primary human colonic epithelial cells and hepatocytes, respectively. Further, quantitative PCR analysis revealed reduced CYLD mRNA expression in most tumor samples compared with non-tumorous tissue. Analysis on protein level confirmed these findings. Functional assays with CYLD transfected cell lines revealed that CYLD expression decreased NF-kappaB activity. Thus, functional relevant loss of CYLD expression may contribute to tumor development and progression, and may provide a new target for therapeutic strategies. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Carcinogenesis
volume
28
issue
1
pages
21 - 27
publisher
Oxford University Press
external identifiers
  • pmid:16774947
  • scopus:33845679104
ISSN
0143-3334
DOI
10.1093/carcin/bgl081
language
English
LU publication?
yes
id
a7db0c0a-c5d7-46a8-accf-944206381223 (old id 1141278)
date added to LUP
2008-08-12 16:03:18
date last changed
2017-10-29 04:28:08
@article{a7db0c0a-c5d7-46a8-accf-944206381223,
  abstract     = {CYLD was originally identified as a tumor suppressor that is mutated in familial cylindromatosis. Recent studies suggested a role for CYLD in nuclear factor-kappaB (NF-kappaB) regulation. NF-kappaB activation has been connected with multiple aspects of oncogenesis but the underlying molecular mechanisms of persistent NF-kappaB activation in tumors remain largely unknown. Thus, we evaluated CYLD transcription in different colon and hepatocellular carcinoma cell lines and tissue samples, respectively. CYLD was downregulated or lost in all tumor cell lines investigated as compared with primary human colonic epithelial cells and hepatocytes, respectively. Further, quantitative PCR analysis revealed reduced CYLD mRNA expression in most tumor samples compared with non-tumorous tissue. Analysis on protein level confirmed these findings. Functional assays with CYLD transfected cell lines revealed that CYLD expression decreased NF-kappaB activity. Thus, functional relevant loss of CYLD expression may contribute to tumor development and progression, and may provide a new target for therapeutic strategies.},
  author       = {Hellerbrand, Claus and Bumes, Elisabeth and Bataille, Frauke and Dietmaier, Wolfgang and Massoumi, Ramin and Bosserhoff, Anja K},
  issn         = {0143-3334},
  language     = {eng},
  number       = {1},
  pages        = {21--27},
  publisher    = {Oxford University Press},
  series       = {Carcinogenesis},
  title        = {Reduced expression of CYLD in human colon and hepatocellular carcinomas},
  url          = {http://dx.doi.org/10.1093/carcin/bgl081},
  volume       = {28},
  year         = {2007},
}