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Expression of cytokines and chemokines in cervical and amniotic fluid: Relationship to histological chorioamnionitis

Holst, Rose-Marie; Laurini, Ricardo LU ; Jacobsson, Bo; Samuelsson, Ellen; Savman, Karin; Doverhag, Christina; Wennerholm, Ulla-Britt and Hagberg, Henrik (2007) In Journal of Maternal-Fetal & Neonatal Medicine 20(12). p.885-893
Abstract
Objective. To correlate cervical and amniotic fluid cytokines and macrophage-related chemokines to the development of histological chorioamnionitis (HCA) in patients with preterm labor (PTL) and preterm prelabor rupture of the membranes (PPROM). Study design. Cervical and amniotic fluid interleukin (IL)-6, IL-8, IL-18, monocyte chemotactic protein (MCP)-1, MCP-2, and MCP-3 from pregnant women (at </=34 weeks of gestation) in PTL (N = 42) were analyzed and related to the subsequent occurrence of HCA or inflammatory signs in the placenta. For the patients with PPROM (N = 30) only amniotic fluid proteins were analyzed. Results. Intra-amniotic levels of IL-6, IL-8, IL-18, MCP-1, and MCP-3 were significantly higher in PTL cases with HCA... (More)
Objective. To correlate cervical and amniotic fluid cytokines and macrophage-related chemokines to the development of histological chorioamnionitis (HCA) in patients with preterm labor (PTL) and preterm prelabor rupture of the membranes (PPROM). Study design. Cervical and amniotic fluid interleukin (IL)-6, IL-8, IL-18, monocyte chemotactic protein (MCP)-1, MCP-2, and MCP-3 from pregnant women (at </=34 weeks of gestation) in PTL (N = 42) were analyzed and related to the subsequent occurrence of HCA or inflammatory signs in the placenta. For the patients with PPROM (N = 30) only amniotic fluid proteins were analyzed. Results. Intra-amniotic levels of IL-6, IL-8, IL-18, MCP-1, and MCP-3 were significantly higher in PTL cases with HCA compared to non-HCA controls, whereas no such relationship was obtained in the PPROM group. Cervical IL-8 and IL-6 (but not IL-18, MCP-1, MCP-2, and MCP-3) in PTL patients was associated with HCA, and at a cut-off level of 10.0 ng/mL cervical IL-8 was a strong predictor of HCA in the PTL cases (sensitivity 100%, specificity 67%, positive predictive value 63%, negative predictive value 100%). The cytokine and chemokine levels in the group with inflammatory signs were generally higher than in controls but lower compared to the concentrations in the HCA group. Conclusions. The amniotic levels of IL-6, IL-8, IL-18, and the CC-chemokines MCP-1 and MCP-3 in PTL patients all predicted HCA, whereas only IL-8 was a clinically useful marker of HCA in the cervical fluid. In addition there is indication that the levels of inflammatory proteins are related to the degree of inflammatory infiltration in placental tissue samples. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Maternal-Fetal & Neonatal Medicine
volume
20
issue
12
pages
885 - 893
publisher
Taylor & Francis
external identifiers
  • pmid:18050018
  • wos:000252150600006
  • scopus:36749077581
ISSN
1476-7058
DOI
10.1080/14767050701752601
language
English
LU publication?
yes
id
ee624cfa-bf5e-49b8-883a-f733da16d50f (old id 1141333)
date added to LUP
2008-08-13 09:47:21
date last changed
2017-11-12 04:07:28
@article{ee624cfa-bf5e-49b8-883a-f733da16d50f,
  abstract     = {Objective. To correlate cervical and amniotic fluid cytokines and macrophage-related chemokines to the development of histological chorioamnionitis (HCA) in patients with preterm labor (PTL) and preterm prelabor rupture of the membranes (PPROM). Study design. Cervical and amniotic fluid interleukin (IL)-6, IL-8, IL-18, monocyte chemotactic protein (MCP)-1, MCP-2, and MCP-3 from pregnant women (at &lt;/=34 weeks of gestation) in PTL (N = 42) were analyzed and related to the subsequent occurrence of HCA or inflammatory signs in the placenta. For the patients with PPROM (N = 30) only amniotic fluid proteins were analyzed. Results. Intra-amniotic levels of IL-6, IL-8, IL-18, MCP-1, and MCP-3 were significantly higher in PTL cases with HCA compared to non-HCA controls, whereas no such relationship was obtained in the PPROM group. Cervical IL-8 and IL-6 (but not IL-18, MCP-1, MCP-2, and MCP-3) in PTL patients was associated with HCA, and at a cut-off level of 10.0 ng/mL cervical IL-8 was a strong predictor of HCA in the PTL cases (sensitivity 100%, specificity 67%, positive predictive value 63%, negative predictive value 100%). The cytokine and chemokine levels in the group with inflammatory signs were generally higher than in controls but lower compared to the concentrations in the HCA group. Conclusions. The amniotic levels of IL-6, IL-8, IL-18, and the CC-chemokines MCP-1 and MCP-3 in PTL patients all predicted HCA, whereas only IL-8 was a clinically useful marker of HCA in the cervical fluid. In addition there is indication that the levels of inflammatory proteins are related to the degree of inflammatory infiltration in placental tissue samples.},
  author       = {Holst, Rose-Marie and Laurini, Ricardo and Jacobsson, Bo and Samuelsson, Ellen and Savman, Karin and Doverhag, Christina and Wennerholm, Ulla-Britt and Hagberg, Henrik},
  issn         = {1476-7058},
  language     = {eng},
  number       = {12},
  pages        = {885--893},
  publisher    = {Taylor & Francis},
  series       = {Journal of Maternal-Fetal & Neonatal Medicine},
  title        = {Expression of cytokines and chemokines in cervical and amniotic fluid: Relationship to histological chorioamnionitis},
  url          = {http://dx.doi.org/10.1080/14767050701752601},
  volume       = {20},
  year         = {2007},
}