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RhoH is important for positive thymocyte selection and T-cell receptor signaling

Dorn, Tatjana; Kuhn, Ursula; Bungartz, Gerd; Stiller, Sebastian; Bauer, Martina; Ellwart, Joachim; Peters, Thorsten; Scharffetter-Kochanek, Karin; Semmrich, Monika LU and Laschinger, Melanie, et al. (2007) In Blood 109(6). p.2346-2355
Abstract
RhoH is a small GTPase expressed only in the hematopoietic system. With the use of mice with targeted disruption of the RhoH gene, we demonstrated that RhoH is crucial for thymocyte maturation during DN3 to DN4 transition and during positive selection. Furthermore, the differentiation and expansion of DN3 and DN4 thymocytes in vitro were severely impaired. These defects corresponded to defective TCR signaling. Although RhoH is not required for TCR-induced activation of ZAP70 and ZAP70-mediated activation of p38, it is crucial for the tyrosine phosphorylation of LAT, PLCgamma1, and Vav1 and for the activation of Erk and calcium influx. These data suggest that RhoH is important for pre-TCR and TCR signaling because it allows the efficient... (More)
RhoH is a small GTPase expressed only in the hematopoietic system. With the use of mice with targeted disruption of the RhoH gene, we demonstrated that RhoH is crucial for thymocyte maturation during DN3 to DN4 transition and during positive selection. Furthermore, the differentiation and expansion of DN3 and DN4 thymocytes in vitro were severely impaired. These defects corresponded to defective TCR signaling. Although RhoH is not required for TCR-induced activation of ZAP70 and ZAP70-mediated activation of p38, it is crucial for the tyrosine phosphorylation of LAT, PLCgamma1, and Vav1 and for the activation of Erk and calcium influx. These data suggest that RhoH is important for pre-TCR and TCR signaling because it allows the efficient interaction of ZAP70 with the LAT signalosome, thus regulating thymocyte development. (Less)
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publishing date
type
Contribution to journal
publication status
published
subject
in
Blood
volume
109
issue
6
pages
2346 - 2355
publisher
American Society of Hematology
external identifiers
  • pmid:17119112
  • scopus:33947204164
ISSN
1528-0020
DOI
10.1182/blood-2006-04-019034
language
English
LU publication?
no
id
5b867096-523f-4c17-9ae2-be4205210624 (old id 1143572)
date added to LUP
2008-07-29 15:47:06
date last changed
2017-11-12 03:21:59
@article{5b867096-523f-4c17-9ae2-be4205210624,
  abstract     = {RhoH is a small GTPase expressed only in the hematopoietic system. With the use of mice with targeted disruption of the RhoH gene, we demonstrated that RhoH is crucial for thymocyte maturation during DN3 to DN4 transition and during positive selection. Furthermore, the differentiation and expansion of DN3 and DN4 thymocytes in vitro were severely impaired. These defects corresponded to defective TCR signaling. Although RhoH is not required for TCR-induced activation of ZAP70 and ZAP70-mediated activation of p38, it is crucial for the tyrosine phosphorylation of LAT, PLCgamma1, and Vav1 and for the activation of Erk and calcium influx. These data suggest that RhoH is important for pre-TCR and TCR signaling because it allows the efficient interaction of ZAP70 with the LAT signalosome, thus regulating thymocyte development.},
  author       = {Dorn, Tatjana and Kuhn, Ursula and Bungartz, Gerd and Stiller, Sebastian and Bauer, Martina and Ellwart, Joachim and Peters, Thorsten and Scharffetter-Kochanek, Karin and Semmrich, Monika and Laschinger, Melanie and Holzmann, Bernhard and Klinkert, Wolfgang E F and Straten, Per Th},
  issn         = {1528-0020},
  language     = {eng},
  number       = {6},
  pages        = {2346--2355},
  publisher    = {American Society of Hematology},
  series       = {Blood},
  title        = {RhoH is important for positive thymocyte selection and T-cell receptor signaling},
  url          = {http://dx.doi.org/10.1182/blood-2006-04-019034},
  volume       = {109},
  year         = {2007},
}