Inflammatory markers in nondiabetic and diabetic rat retinas exposed to ischemia followed by reperfusion.
(2008) In Retina 28(4). p.645-652- Abstract
- PURPOSE:: To examine the retinal inflammatory response to ischemia-reperfusion in nondiabetic and diabetic rats injected with either an omega-3-polyunsaturated fatty acid (docosahexaenoic acid [DHA]) or a 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitor (pravastatin). METHODS:: Diabetes was induced by an intraperitoneal injection of streptozocin, and retinal ischemia was induced by ligation of the optic nerve and vessels, followed by reperfusion for 1 hour or 24 hours. Five minutes before surgery, an intravenous injection of DHA, pravastatin, or vehicle (ethanol) was administered. The mRNA expressions of tumor necrosis factor (TNF)-alpha, interleukin (IL)-6, caspase-1, IL-1beta, P-selectin, vascular cellular adhesion molecule... (More)
- PURPOSE:: To examine the retinal inflammatory response to ischemia-reperfusion in nondiabetic and diabetic rats injected with either an omega-3-polyunsaturated fatty acid (docosahexaenoic acid [DHA]) or a 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitor (pravastatin). METHODS:: Diabetes was induced by an intraperitoneal injection of streptozocin, and retinal ischemia was induced by ligation of the optic nerve and vessels, followed by reperfusion for 1 hour or 24 hours. Five minutes before surgery, an intravenous injection of DHA, pravastatin, or vehicle (ethanol) was administered. The mRNA expressions of tumor necrosis factor (TNF)-alpha, interleukin (IL)-6, caspase-1, IL-1beta, P-selectin, vascular cellular adhesion molecule (VCAM)-1, and intercellular adhesion molecule (ICAM)-1 were compared between ischemic and nonischemic retinas as well as diabetic and nondiabetic nonischemic retinas. RESULTS:: Ischemia induced increased expressions of TNF-alpha (P </= 0.012), IL-1beta (P </= 0.017), ICAM-1 (P </= 0.025), and IL-6 (P = 0.012), and diabetes induced increased expression of caspase-1 (P </= 0.046), VCAM-1 (P </= 0.027), ICAM-1 (P </= 0.016), IL-1beta (P = 0.016), and IL-6 (P = 0.041). Ischemia plus diabetes did not increase these findings significantly. DHA and pravastatin had some inhibitory effects in diabetic rats (P </= 0.037). CONCLUSIONS:: Inflammation triggered by ischemia-reperfusion may play a role in diabetic retinopathy. Intervention on lipid-based structures by omega-3-polyunsaturated fatty acids or statins seemed to have beneficial effects on inflammation in diabetes. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1147607
- author
- Gustavsson, Carin LU ; Agardh, Carl-David LU ; Hagert, Per LU and Agardh, Elisabet LU
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Retina
- volume
- 28
- issue
- 4
- pages
- 645 - 652
- publisher
- Lippincott Williams & Wilkins
- external identifiers
-
- wos:000255370800017
- pmid:18398369
- scopus:42549101711
- ISSN
- 0275-004X
- DOI
- 10.1097/IAE.0b013e31815ec32d
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Unit on Vascular Diabetic Complications (013241510)
- id
- 75fb11cd-9b79-4275-9d9c-4adabc269474 (old id 1147607)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/18398369?dopt=Abstract
- date added to LUP
- 2016-04-04 09:17:48
- date last changed
- 2022-03-07 23:59:20
@article{75fb11cd-9b79-4275-9d9c-4adabc269474, abstract = {{PURPOSE:: To examine the retinal inflammatory response to ischemia-reperfusion in nondiabetic and diabetic rats injected with either an omega-3-polyunsaturated fatty acid (docosahexaenoic acid [DHA]) or a 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitor (pravastatin). METHODS:: Diabetes was induced by an intraperitoneal injection of streptozocin, and retinal ischemia was induced by ligation of the optic nerve and vessels, followed by reperfusion for 1 hour or 24 hours. Five minutes before surgery, an intravenous injection of DHA, pravastatin, or vehicle (ethanol) was administered. The mRNA expressions of tumor necrosis factor (TNF)-alpha, interleukin (IL)-6, caspase-1, IL-1beta, P-selectin, vascular cellular adhesion molecule (VCAM)-1, and intercellular adhesion molecule (ICAM)-1 were compared between ischemic and nonischemic retinas as well as diabetic and nondiabetic nonischemic retinas. RESULTS:: Ischemia induced increased expressions of TNF-alpha (P </= 0.012), IL-1beta (P </= 0.017), ICAM-1 (P </= 0.025), and IL-6 (P = 0.012), and diabetes induced increased expression of caspase-1 (P </= 0.046), VCAM-1 (P </= 0.027), ICAM-1 (P </= 0.016), IL-1beta (P = 0.016), and IL-6 (P = 0.041). Ischemia plus diabetes did not increase these findings significantly. DHA and pravastatin had some inhibitory effects in diabetic rats (P </= 0.037). CONCLUSIONS:: Inflammation triggered by ischemia-reperfusion may play a role in diabetic retinopathy. Intervention on lipid-based structures by omega-3-polyunsaturated fatty acids or statins seemed to have beneficial effects on inflammation in diabetes.}}, author = {{Gustavsson, Carin and Agardh, Carl-David and Hagert, Per and Agardh, Elisabet}}, issn = {{0275-004X}}, language = {{eng}}, number = {{4}}, pages = {{645--652}}, publisher = {{Lippincott Williams & Wilkins}}, series = {{Retina}}, title = {{Inflammatory markers in nondiabetic and diabetic rat retinas exposed to ischemia followed by reperfusion.}}, url = {{http://dx.doi.org/10.1097/IAE.0b013e31815ec32d}}, doi = {{10.1097/IAE.0b013e31815ec32d}}, volume = {{28}}, year = {{2008}}, }