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Field-Dependent DNA Mobility in 20 nm High Nanoslits.

Salieb-Beugelaar, Georgette ; Teapal, Juliane ; Nieuwkasteele, Jan ; Wijnperlé, Daniël ; Tegenfeldt, Jonas LU orcid ; Lisdat, Fred ; van den Berg, Albert and Eijkel, Jan (2008) In Nano Letters 8(7). p.1785-1790
Abstract
The transport behavior of lambda-DNA (48 kbp) in fused silica nanoslits is investigated upon application of electrical fields of different strengths. The slit dimensions are 20 nm in height, 3 microm in width, and 500 microm in length. With fields of 30 kV/m or below, the molecules move fluently through the slits, while at higher electrical fields, the DNA molecules move intermittently, resulting in a strongly reduced mobility. We propose that the behavior can be explained by mechanical and/or field-induced dielectrophoretic DNA trapping due to the surface roughness in the nanoslits. The observation of preferential pathways and trapping sites of the lambda-DNA molecules through the nanoslits supports this hypothesis and indicates that the... (More)
The transport behavior of lambda-DNA (48 kbp) in fused silica nanoslits is investigated upon application of electrical fields of different strengths. The slit dimensions are 20 nm in height, 3 microm in width, and 500 microm in length. With fields of 30 kV/m or below, the molecules move fluently through the slits, while at higher electrical fields, the DNA molecules move intermittently, resulting in a strongly reduced mobility. We propose that the behavior can be explained by mechanical and/or field-induced dielectrophoretic DNA trapping due to the surface roughness in the nanoslits. The observation of preferential pathways and trapping sites of the lambda-DNA molecules through the nanoslits supports this hypothesis and indicates that the classical viscous friction models to explain the DNA movement in nanoslits needs to be modified to include these effects. Preliminary experiments with the smaller XbaI-digested litmus-DNA (2.8 kbp) show that the behavior is size-dependent, suggesting that the high field electrophoresis in nanoslits can be used for DNA separation. (Less)
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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Nano Letters
volume
8
issue
7
pages
1785 - 1790
publisher
The American Chemical Society (ACS)
external identifiers
  • wos:000257504500001
  • pmid:18393468
  • scopus:53149084945
  • pmid:18393468
ISSN
1530-6992
DOI
10.1021/nl080300v
language
English
LU publication?
yes
id
b33c00f3-6318-41e9-9d47-336a3c0e7a3c (old id 1147669)
date added to LUP
2016-04-01 14:56:56
date last changed
2022-01-28 03:15:39
@article{b33c00f3-6318-41e9-9d47-336a3c0e7a3c,
  abstract     = {{The transport behavior of lambda-DNA (48 kbp) in fused silica nanoslits is investigated upon application of electrical fields of different strengths. The slit dimensions are 20 nm in height, 3 microm in width, and 500 microm in length. With fields of 30 kV/m or below, the molecules move fluently through the slits, while at higher electrical fields, the DNA molecules move intermittently, resulting in a strongly reduced mobility. We propose that the behavior can be explained by mechanical and/or field-induced dielectrophoretic DNA trapping due to the surface roughness in the nanoslits. The observation of preferential pathways and trapping sites of the lambda-DNA molecules through the nanoslits supports this hypothesis and indicates that the classical viscous friction models to explain the DNA movement in nanoslits needs to be modified to include these effects. Preliminary experiments with the smaller XbaI-digested litmus-DNA (2.8 kbp) show that the behavior is size-dependent, suggesting that the high field electrophoresis in nanoslits can be used for DNA separation.}},
  author       = {{Salieb-Beugelaar, Georgette and Teapal, Juliane and Nieuwkasteele, Jan and Wijnperlé, Daniël and Tegenfeldt, Jonas and Lisdat, Fred and van den Berg, Albert and Eijkel, Jan}},
  issn         = {{1530-6992}},
  language     = {{eng}},
  number       = {{7}},
  pages        = {{1785--1790}},
  publisher    = {{The American Chemical Society (ACS)}},
  series       = {{Nano Letters}},
  title        = {{Field-Dependent DNA Mobility in 20 nm High Nanoslits.}},
  url          = {{http://dx.doi.org/10.1021/nl080300v}},
  doi          = {{10.1021/nl080300v}},
  volume       = {{8}},
  year         = {{2008}},
}