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Nonaqueous capillary electrophoresis for analysis of the ethanol consumption biomarker phosphatidylethanol.

Varga, Arthur LU and Nilsson, Staffan LU (2008) In Electrophoresis 29(8). p.1667-1671
Abstract
Nonaqueous CE (NACE) methodology was developed for the separation and determination of phosphatidylethanol (Peth), a new biomarker of ethanol intake. Peth is an abnormal phospholipid formed in cell membranes only in the presence of ethanol, via the transphosphatidylation reaction of phospholipase D. The NACE separation medium consisted of 80 mM ammonium acetate in 50% ACN, 33% 2-propanol, 12% hexane and 5% methanol. A stacking effect was obtained by reducing the concentration of ammonium acetate in the separation medium for all injected samples. The LOD was estimated to 1 muM (5.6 fmol) of Peth with conventional UV detection, equalling 0.4 mumol/L blood. Peth was successfully determined in extracts of human blood samples. Separation of... (More)
Nonaqueous CE (NACE) methodology was developed for the separation and determination of phosphatidylethanol (Peth), a new biomarker of ethanol intake. Peth is an abnormal phospholipid formed in cell membranes only in the presence of ethanol, via the transphosphatidylation reaction of phospholipase D. The NACE separation medium consisted of 80 mM ammonium acetate in 50% ACN, 33% 2-propanol, 12% hexane and 5% methanol. A stacking effect was obtained by reducing the concentration of ammonium acetate in the separation medium for all injected samples. The LOD was estimated to 1 muM (5.6 fmol) of Peth with conventional UV detection, equalling 0.4 mumol/L blood. Peth was successfully determined in extracts of human blood samples. Separation of Peth from other blood lipids in the lipid extract sample was performed in 5 min. The method facilitates smaller sample volumes and performs about ten times faster compared to earlier chromatographical methods. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Electrophoresis
volume
29
issue
8
pages
1667 - 1671
publisher
John Wiley & Sons
external identifiers
  • wos:000255703100011
  • pmid:18383031
  • scopus:42649089187
ISSN
0173-0835
DOI
10.1002/elps.200700548
language
English
LU publication?
yes
id
52c0c30a-aa70-4746-ac46-25ab9235249d (old id 1147872)
date added to LUP
2009-03-09 12:44:34
date last changed
2017-10-22 03:41:53
@article{52c0c30a-aa70-4746-ac46-25ab9235249d,
  abstract     = {Nonaqueous CE (NACE) methodology was developed for the separation and determination of phosphatidylethanol (Peth), a new biomarker of ethanol intake. Peth is an abnormal phospholipid formed in cell membranes only in the presence of ethanol, via the transphosphatidylation reaction of phospholipase D. The NACE separation medium consisted of 80 mM ammonium acetate in 50% ACN, 33% 2-propanol, 12% hexane and 5% methanol. A stacking effect was obtained by reducing the concentration of ammonium acetate in the separation medium for all injected samples. The LOD was estimated to 1 muM (5.6 fmol) of Peth with conventional UV detection, equalling 0.4 mumol/L blood. Peth was successfully determined in extracts of human blood samples. Separation of Peth from other blood lipids in the lipid extract sample was performed in 5 min. The method facilitates smaller sample volumes and performs about ten times faster compared to earlier chromatographical methods.},
  author       = {Varga, Arthur and Nilsson, Staffan},
  issn         = {0173-0835},
  language     = {eng},
  number       = {8},
  pages        = {1667--1671},
  publisher    = {John Wiley & Sons},
  series       = {Electrophoresis},
  title        = {Nonaqueous capillary electrophoresis for analysis of the ethanol consumption biomarker phosphatidylethanol.},
  url          = {http://dx.doi.org/10.1002/elps.200700548},
  volume       = {29},
  year         = {2008},
}