Advanced

Calmodulin Binding to the Polybasic C-Termini of STIM Proteins Involved in Store-Operated Calcium Entry.

Bauer, Mikael LU ; O'Connell, David; Cahill, Dolores and Linse, Sara LU (2008) In Biochemistry 47(23). p.6089-6091
Abstract
Translocation of STIM1 and STIM2 from the endoplasmic reticulum to the plasma membrane is a key step in store-operated calcium entry in the cell. We show by isothermal titration calorimetry that calmodulin binds in a calcium-dependent manner to the polybasic C-termini of STIM1 and STIM2, a region critical for their translocation to the plasma membrane ( K D </= 1 microM in calcium). HSQC NMR spectroscopy shows this interaction is in the fast exchange regime. By binding STIM1 and STIM2, calmodulin may regulate store refilling, thereby ensuring the maintenance of its own action in intracellular signaling.
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biochemistry
volume
47
issue
23
pages
6089 - 6091
publisher
The American Chemical Society
external identifiers
  • wos:000256405800001
  • pmid:18484746
  • scopus:44949241711
ISSN
0006-2960
DOI
10.1021/bi800496a
language
English
LU publication?
yes
id
b9d2365a-127e-4594-ba0f-c97b460d2fa1 (old id 1154046)
date added to LUP
2008-10-08 12:17:33
date last changed
2017-06-25 03:28:54
@article{b9d2365a-127e-4594-ba0f-c97b460d2fa1,
  abstract     = {Translocation of STIM1 and STIM2 from the endoplasmic reticulum to the plasma membrane is a key step in store-operated calcium entry in the cell. We show by isothermal titration calorimetry that calmodulin binds in a calcium-dependent manner to the polybasic C-termini of STIM1 and STIM2, a region critical for their translocation to the plasma membrane ( K D &lt;/= 1 microM in calcium). HSQC NMR spectroscopy shows this interaction is in the fast exchange regime. By binding STIM1 and STIM2, calmodulin may regulate store refilling, thereby ensuring the maintenance of its own action in intracellular signaling.},
  author       = {Bauer, Mikael and O'Connell, David and Cahill, Dolores and Linse, Sara},
  issn         = {0006-2960},
  language     = {eng},
  number       = {23},
  pages        = {6089--6091},
  publisher    = {The American Chemical Society},
  series       = {Biochemistry},
  title        = {Calmodulin Binding to the Polybasic C-Termini of STIM Proteins Involved in Store-Operated Calcium Entry.},
  url          = {http://dx.doi.org/10.1021/bi800496a},
  volume       = {47},
  year         = {2008},
}