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3,4-dge in peritoneal dialysis fluids cannot be found in plasma after infusion into the peritoneal cavity.

Erixon, Martin LU ; Wieslander, Anders; Lindén, Torbjörn; Carlsson, Ola; Jönsson, Jan Åke LU ; Simonsen, Ole LU and Kjellstrand, Per (2008) In Peritoneal Dialysis International 28(3). p.277-282
Abstract
OBJECTIVE: Glucose degradation products (GDPs) are important in the outcome of peritoneal dialysis (PD) treatment. 3,4-dideoxyglucosone-3-ene (3,4-DGE) is the most cytotoxic GDP found in conventionally manufactured fluids and may, in addition, be recruited from 3-deoxyglucosone (3-DG). It is not known what happens with those GDPs in patients during PD. The aim of this study was to investigate if the 3,4-DGE and 3-DG in PD fluids can be found in plasma during treatment. DESIGN: PD patients were dialyzed with a conventional PD fluid containing 43 mumol/L 3,4-DGE and 281 mumol/L 3-DG. Parallel experiments were performed in rats as well as in vitro with human plasma. The rats were dialyzed with a PD fluid containing 100 mumol/L 3,4-DGE and 200... (More)
OBJECTIVE: Glucose degradation products (GDPs) are important in the outcome of peritoneal dialysis (PD) treatment. 3,4-dideoxyglucosone-3-ene (3,4-DGE) is the most cytotoxic GDP found in conventionally manufactured fluids and may, in addition, be recruited from 3-deoxyglucosone (3-DG). It is not known what happens with those GDPs in patients during PD. The aim of this study was to investigate if the 3,4-DGE and 3-DG in PD fluids can be found in plasma during treatment. DESIGN: PD patients were dialyzed with a conventional PD fluid containing 43 mumol/L 3,4-DGE and 281 mumol/L 3-DG. Parallel experiments were performed in rats as well as in vitro with human plasma. The rats were dialyzed with a PD fluid containing 100 mumol/L 3,4-DGE and 200 mumol/L 3-DG. RESULTS: The concentration of 3,4-DGE in the peritoneum decreased at a much higher rate than 3-DG during the dwell. 3,4-DGE was not, however, detected in the plasma of patients or rats during dialysis. The concentration of 3-DG in plasma peaked shortly after infusion of the fluid to the peritoneal cavity. The concentration of 3,4-DGE during experimental incubation in plasma decreased rapidly, while the concentration of 3-DG decreased only 10% as rapidly or less. CONCLUSION: 3,4-DGE could not be detected in plasma from either PD patients or rats during dialysis. This is presumably due to its high reactivity. 3-DG may, on the other hand, pass through the membrane and be detected in the blood. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
plasma, advanced glycation end products, 3-DG, PD fluid, 4-DGE, 3, glucose degradation products (GDPs)
in
Peritoneal Dialysis International
volume
28
issue
3
pages
277 - 282
publisher
Multimed Inc.
external identifiers
  • wos:000256889700015
  • pmid:18474921
  • scopus:48949117517
ISSN
1718-4304
language
English
LU publication?
yes
id
e4795893-3321-4a59-8e22-48a1ed6de337 (old id 1154258)
alternative location
http://www.pdiconnect.com/cgi/content/abstract/28/3/277
date added to LUP
2008-10-08 11:58:13
date last changed
2017-01-01 06:25:11
@article{e4795893-3321-4a59-8e22-48a1ed6de337,
  abstract     = {OBJECTIVE: Glucose degradation products (GDPs) are important in the outcome of peritoneal dialysis (PD) treatment. 3,4-dideoxyglucosone-3-ene (3,4-DGE) is the most cytotoxic GDP found in conventionally manufactured fluids and may, in addition, be recruited from 3-deoxyglucosone (3-DG). It is not known what happens with those GDPs in patients during PD. The aim of this study was to investigate if the 3,4-DGE and 3-DG in PD fluids can be found in plasma during treatment. DESIGN: PD patients were dialyzed with a conventional PD fluid containing 43 mumol/L 3,4-DGE and 281 mumol/L 3-DG. Parallel experiments were performed in rats as well as in vitro with human plasma. The rats were dialyzed with a PD fluid containing 100 mumol/L 3,4-DGE and 200 mumol/L 3-DG. RESULTS: The concentration of 3,4-DGE in the peritoneum decreased at a much higher rate than 3-DG during the dwell. 3,4-DGE was not, however, detected in the plasma of patients or rats during dialysis. The concentration of 3-DG in plasma peaked shortly after infusion of the fluid to the peritoneal cavity. The concentration of 3,4-DGE during experimental incubation in plasma decreased rapidly, while the concentration of 3-DG decreased only 10% as rapidly or less. CONCLUSION: 3,4-DGE could not be detected in plasma from either PD patients or rats during dialysis. This is presumably due to its high reactivity. 3-DG may, on the other hand, pass through the membrane and be detected in the blood.},
  author       = {Erixon, Martin and Wieslander, Anders and Lindén, Torbjörn and Carlsson, Ola and Jönsson, Jan Åke and Simonsen, Ole and Kjellstrand, Per},
  issn         = {1718-4304},
  keyword      = {plasma,advanced glycation end products,3-DG,PD fluid,4-DGE,3,glucose degradation products (GDPs)},
  language     = {eng},
  number       = {3},
  pages        = {277--282},
  publisher    = {Multimed Inc.},
  series       = {Peritoneal Dialysis International},
  title        = {3,4-dge in peritoneal dialysis fluids cannot be found in plasma after infusion into the peritoneal cavity.},
  volume       = {28},
  year         = {2008},
}