Advanced

UVB-induced inflammation gives increased d-dopachrome tautomerase activity in blister fluid which correlates with macrophage migration inhibitory factor.

Sonesson, Björn C LU ; Rosengren, Evald LU ; Hansson, Ann-Sofie and Hansson, Christer LU (2003) In Experimental Dermatology 12(3). p.278-282
Abstract
UVB light was used to induce an experimental inflammation in normal human skin in order to investigate its correlation with the activity of the newly described enzyme d-dopachrome tautomerase (DDT) in the fluid of experimental blisters. Macrophage migration inhibitory factor (MIF) activity was determined as a closely related marker of inflammation. DDT and MIF activities were demonstrated in blister fluids in all 10 healthy subjects. All but one of these subjects showed increased activity of DDT and MIF after three minimal erythemal doses (MED) of UVB. The mean activity of DDT increased approximately twofold and the mean activity of MIF also increased twofold after UVB in our experimental model. We found a strong correlation between DDT... (More)
UVB light was used to induce an experimental inflammation in normal human skin in order to investigate its correlation with the activity of the newly described enzyme d-dopachrome tautomerase (DDT) in the fluid of experimental blisters. Macrophage migration inhibitory factor (MIF) activity was determined as a closely related marker of inflammation. DDT and MIF activities were demonstrated in blister fluids in all 10 healthy subjects. All but one of these subjects showed increased activity of DDT and MIF after three minimal erythemal doses (MED) of UVB. The mean activity of DDT increased approximately twofold and the mean activity of MIF also increased twofold after UVB in our experimental model. We found a strong correlation between DDT and MIF activities. The presence of DDT in epidermis and its increase at UV irradiation was confirmed by immunohistochemical studies. In this study, DDT is for the first time demonstrated in the skin. It is also the first time DDT can be related to inflammation, and its covariation with MIF strengthens this observation. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Experimental Dermatology
volume
12
issue
3
pages
278 - 282
publisher
Wiley-Blackwell
external identifiers
  • pmid:12823441
  • wos:000183599000007
  • scopus:0042674031
ISSN
0906-6705
DOI
10.1034/j.1600-0625.2003.120307.x
language
English
LU publication?
yes
id
e9fc64d5-eb27-47cd-9b61-7a8f2f790c57 (old id 115723)
alternative location
http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=Retrieve&db=PubMed&list_uids=12823441&dopt=Abstract
date added to LUP
2007-07-03 09:13:54
date last changed
2018-05-29 09:54:10
@article{e9fc64d5-eb27-47cd-9b61-7a8f2f790c57,
  abstract     = {UVB light was used to induce an experimental inflammation in normal human skin in order to investigate its correlation with the activity of the newly described enzyme d-dopachrome tautomerase (DDT) in the fluid of experimental blisters. Macrophage migration inhibitory factor (MIF) activity was determined as a closely related marker of inflammation. DDT and MIF activities were demonstrated in blister fluids in all 10 healthy subjects. All but one of these subjects showed increased activity of DDT and MIF after three minimal erythemal doses (MED) of UVB. The mean activity of DDT increased approximately twofold and the mean activity of MIF also increased twofold after UVB in our experimental model. We found a strong correlation between DDT and MIF activities. The presence of DDT in epidermis and its increase at UV irradiation was confirmed by immunohistochemical studies. In this study, DDT is for the first time demonstrated in the skin. It is also the first time DDT can be related to inflammation, and its covariation with MIF strengthens this observation.},
  author       = {Sonesson, Björn C and Rosengren, Evald and Hansson, Ann-Sofie and Hansson, Christer},
  issn         = {0906-6705},
  language     = {eng},
  number       = {3},
  pages        = {278--282},
  publisher    = {Wiley-Blackwell},
  series       = {Experimental Dermatology},
  title        = {UVB-induced inflammation gives increased d-dopachrome tautomerase activity in blister fluid which correlates with macrophage migration inhibitory factor.},
  url          = {http://dx.doi.org/10.1034/j.1600-0625.2003.120307.x},
  volume       = {12},
  year         = {2003},
}