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Morphological, genetic and functional variability of a T-cell hybridoma line.

Dzhambazov, Balik LU ; Teneva, I; Koleva, L; Asparuhova, D and Popov, N (2003) In Folia Biologica 49(2). p.87-94
Abstract
The variability in the morphology, modal number of chromosomes, TCR expression and functional reactivity of a CII-specific T-cell hybridoma at continuous subcultivation have been investigated. As the number of passages increased, besides the oval semiadherent cells (normal phenotype), fibroblast-like cells (transformed phenotype) were also observed. The two cell subpopulations differed in their karyotype characteristic, as well as in their functional reactivity. The cell population with a normal phenotype was characterized by a tetramodal number of chromosomes (30, 40, 48 and 70) and trisomies of chromosomes 6 and 14, while the cell population with a transformed phenotype was characterized by a trimodal number of chromosomes (11, 68 and... (More)
The variability in the morphology, modal number of chromosomes, TCR expression and functional reactivity of a CII-specific T-cell hybridoma at continuous subcultivation have been investigated. As the number of passages increased, besides the oval semiadherent cells (normal phenotype), fibroblast-like cells (transformed phenotype) were also observed. The two cell subpopulations differed in their karyotype characteristic, as well as in their functional reactivity. The cell population with a normal phenotype was characterized by a tetramodal number of chromosomes (30, 40, 48 and 70) and trisomies of chromosomes 6 and 14, while the cell population with a transformed phenotype was characterized by a trimodal number of chromosomes (11, 68 and 74) and trisomy of chromosome 12. A nullisomy of sex chromosomes was established in both types of cells. In the initial passages of subcultivation, 73.04% of the cells with a normal morphological phenotype expressed TCR-CD3 complexes on their surface and possessed high functional reactivity. After a two-week subcultivation, the values of these indices went down considerably: 46.11% of the cells expressed functional TCR-CD3 complexes, as a result of which their functional reactivity decreased. Only 2.71% of the cells with a transformed morphological phenotype expressed functional TCR-CD3 complexes on their surface. In these cells, a total loss of reactivity towards the specific antigens was established. The achieved results show that at continuous subcultivation the T-cell hybridomas are unstable, and with the increase in the number of passages there appear chromosome rearrangements, leading to loss of their functional reactivity. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
in
Folia Biologica
volume
49
issue
2
pages
87 - 94
publisher
Academy of Sciences of the Czech Republic
external identifiers
  • wos:000182646000006
  • pmid:12779018
  • scopus:0038634874
ISSN
0015-5500
language
English
LU publication?
yes
id
38f42d25-4ca0-4578-a02c-955c8362fb03 (old id 116244)
alternative location
http://www.img.cas.cz/fb/pdf/2003-49-87-94.pdf
date added to LUP
2007-07-11 13:32:20
date last changed
2018-05-29 11:00:29
@article{38f42d25-4ca0-4578-a02c-955c8362fb03,
  abstract     = {The variability in the morphology, modal number of chromosomes, TCR expression and functional reactivity of a CII-specific T-cell hybridoma at continuous subcultivation have been investigated. As the number of passages increased, besides the oval semiadherent cells (normal phenotype), fibroblast-like cells (transformed phenotype) were also observed. The two cell subpopulations differed in their karyotype characteristic, as well as in their functional reactivity. The cell population with a normal phenotype was characterized by a tetramodal number of chromosomes (30, 40, 48 and 70) and trisomies of chromosomes 6 and 14, while the cell population with a transformed phenotype was characterized by a trimodal number of chromosomes (11, 68 and 74) and trisomy of chromosome 12. A nullisomy of sex chromosomes was established in both types of cells. In the initial passages of subcultivation, 73.04% of the cells with a normal morphological phenotype expressed TCR-CD3 complexes on their surface and possessed high functional reactivity. After a two-week subcultivation, the values of these indices went down considerably: 46.11% of the cells expressed functional TCR-CD3 complexes, as a result of which their functional reactivity decreased. Only 2.71% of the cells with a transformed morphological phenotype expressed functional TCR-CD3 complexes on their surface. In these cells, a total loss of reactivity towards the specific antigens was established. The achieved results show that at continuous subcultivation the T-cell hybridomas are unstable, and with the increase in the number of passages there appear chromosome rearrangements, leading to loss of their functional reactivity.},
  author       = {Dzhambazov, Balik and Teneva, I and Koleva, L and Asparuhova, D and Popov, N},
  issn         = {0015-5500},
  language     = {eng},
  number       = {2},
  pages        = {87--94},
  publisher    = {Academy of Sciences of the Czech Republic},
  series       = {Folia Biologica},
  title        = {Morphological, genetic and functional variability of a T-cell hybridoma line.},
  volume       = {49},
  year         = {2003},
}