Morphological, genetic and functional variability of a T-cell hybridoma line.
(2003) In Folia Biologica 49(2). p.87-94- Abstract
- The variability in the morphology, modal number of chromosomes, TCR expression and functional reactivity of a CII-specific T-cell hybridoma at continuous subcultivation have been investigated. As the number of passages increased, besides the oval semiadherent cells (normal phenotype), fibroblast-like cells (transformed phenotype) were also observed. The two cell subpopulations differed in their karyotype characteristic, as well as in their functional reactivity. The cell population with a normal phenotype was characterized by a tetramodal number of chromosomes (30, 40, 48 and 70) and trisomies of chromosomes 6 and 14, while the cell population with a transformed phenotype was characterized by a trimodal number of chromosomes (11, 68 and... (More)
- The variability in the morphology, modal number of chromosomes, TCR expression and functional reactivity of a CII-specific T-cell hybridoma at continuous subcultivation have been investigated. As the number of passages increased, besides the oval semiadherent cells (normal phenotype), fibroblast-like cells (transformed phenotype) were also observed. The two cell subpopulations differed in their karyotype characteristic, as well as in their functional reactivity. The cell population with a normal phenotype was characterized by a tetramodal number of chromosomes (30, 40, 48 and 70) and trisomies of chromosomes 6 and 14, while the cell population with a transformed phenotype was characterized by a trimodal number of chromosomes (11, 68 and 74) and trisomy of chromosome 12. A nullisomy of sex chromosomes was established in both types of cells. In the initial passages of subcultivation, 73.04% of the cells with a normal morphological phenotype expressed TCR-CD3 complexes on their surface and possessed high functional reactivity. After a two-week subcultivation, the values of these indices went down considerably: 46.11% of the cells expressed functional TCR-CD3 complexes, as a result of which their functional reactivity decreased. Only 2.71% of the cells with a transformed morphological phenotype expressed functional TCR-CD3 complexes on their surface. In these cells, a total loss of reactivity towards the specific antigens was established. The achieved results show that at continuous subcultivation the T-cell hybridomas are unstable, and with the increase in the number of passages there appear chromosome rearrangements, leading to loss of their functional reactivity. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/116244
- author
- Dzhambazov, Balik LU ; Teneva, I ; Koleva, L ; Asparuhova, D and Popov, N
- organization
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Folia Biologica
- volume
- 49
- issue
- 2
- pages
- 87 - 94
- publisher
- Academy of Sciences of the Czech Republic
- external identifiers
-
- wos:000182646000006
- pmid:12779018
- scopus:0038634874
- ISSN
- 0015-5500
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Medical Inflammation Research (013212019)
- id
- 38f42d25-4ca0-4578-a02c-955c8362fb03 (old id 116244)
- alternative location
- http://www.img.cas.cz/fb/pdf/2003-49-87-94.pdf
- date added to LUP
- 2016-04-01 17:07:35
- date last changed
- 2022-01-29 00:33:48
@article{38f42d25-4ca0-4578-a02c-955c8362fb03, abstract = {{The variability in the morphology, modal number of chromosomes, TCR expression and functional reactivity of a CII-specific T-cell hybridoma at continuous subcultivation have been investigated. As the number of passages increased, besides the oval semiadherent cells (normal phenotype), fibroblast-like cells (transformed phenotype) were also observed. The two cell subpopulations differed in their karyotype characteristic, as well as in their functional reactivity. The cell population with a normal phenotype was characterized by a tetramodal number of chromosomes (30, 40, 48 and 70) and trisomies of chromosomes 6 and 14, while the cell population with a transformed phenotype was characterized by a trimodal number of chromosomes (11, 68 and 74) and trisomy of chromosome 12. A nullisomy of sex chromosomes was established in both types of cells. In the initial passages of subcultivation, 73.04% of the cells with a normal morphological phenotype expressed TCR-CD3 complexes on their surface and possessed high functional reactivity. After a two-week subcultivation, the values of these indices went down considerably: 46.11% of the cells expressed functional TCR-CD3 complexes, as a result of which their functional reactivity decreased. Only 2.71% of the cells with a transformed morphological phenotype expressed functional TCR-CD3 complexes on their surface. In these cells, a total loss of reactivity towards the specific antigens was established. The achieved results show that at continuous subcultivation the T-cell hybridomas are unstable, and with the increase in the number of passages there appear chromosome rearrangements, leading to loss of their functional reactivity.}}, author = {{Dzhambazov, Balik and Teneva, I and Koleva, L and Asparuhova, D and Popov, N}}, issn = {{0015-5500}}, language = {{eng}}, number = {{2}}, pages = {{87--94}}, publisher = {{Academy of Sciences of the Czech Republic}}, series = {{Folia Biologica}}, title = {{Morphological, genetic and functional variability of a T-cell hybridoma line.}}, url = {{http://www.img.cas.cz/fb/pdf/2003-49-87-94.pdf}}, volume = {{49}}, year = {{2003}}, }