Permanent Distal Occlusion of Middle Cerebral Artery in Rat Causes Local Increased ETB, 5-HT1B and AT(1) Receptor-Mediated Contractility Downstream of Occlusion
(2013) In Journal of Vascular Research 50(5). p.396-409- Abstract
- Background/Aims: In response to experimental stroke, a characteristic functional and expressional upregulation of contractile G-protein-coupled receptors has been uncovered in the affected cerebral vasculature; however, the mechanism initiating this phenomenon remains unknown. Methods: Using a model of permanent distal occlusion of rat middle cerebral arteries, we investigated whether there was a regional difference in receptor-mediated contractility of segments located upstream and downstream of the occlusion site. The contractile response to endothelin, angiotensin and 5-hydroxytryptamine receptor stimulation was studied by sensitive wire myograph. Results: Only downstream segments exhibited an augmented contractile response to... (More)
- Background/Aims: In response to experimental stroke, a characteristic functional and expressional upregulation of contractile G-protein-coupled receptors has been uncovered in the affected cerebral vasculature; however, the mechanism initiating this phenomenon remains unknown. Methods: Using a model of permanent distal occlusion of rat middle cerebral arteries, we investigated whether there was a regional difference in receptor-mediated contractility of segments located upstream and downstream of the occlusion site. The contractile response to endothelin, angiotensin and 5-hydroxytryptamine receptor stimulation was studied by sensitive wire myograph. Results: Only downstream segments exhibited an augmented contractile response to stimulation with each of the three ligands, with the response towards sarafotoxin 6c being especially augmented compared to sham, upstream and contralateral controls. This functional increase did not seem to relate to ischemic tissue damage, inflammatory cell infiltration or the element of reperfusion. Interestingly, immunohistochemistry did not show any difference in the level of immunoreactivity towards endothelin B (ETB) receptors between groups. Conclusion: Single artery occlusion without significant visible infarct resulted in locally increased ETB, angiotensin type 1 and 5-hydroxytryptamine 1B receptor-mediated contractile responses only in segments located downstream of the occlusion site. This suggests lack of wall stress as an initiating trigger leading to regulation of contractile response after cerebral stroke. (C) 2013 S. Karger AG, Basel (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4212500
- author
- Rasmussen, Marianne N. P. ; Hornbak, Malene ; Larsen, Stine S. ; Sheykhzade, Majid and Edvinsson, Lars LU
- organization
- publishing date
- 2013
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- 5-HT1B receptor, AT(1) receptor, ETB receptor, Middle cerebral artery, Permanent occlusion, Perfusion pressure, Wall tension
- in
- Journal of Vascular Research
- volume
- 50
- issue
- 5
- pages
- 396 - 409
- publisher
- Karger
- external identifiers
-
- wos:000326362700004
- scopus:84886794102
- pmid:23988741
- ISSN
- 1423-0135
- DOI
- 10.1159/000354242
- language
- English
- LU publication?
- yes
- id
- 116d9549-e76e-4c01-9e1d-3f4f2f9659c6 (old id 4212500)
- date added to LUP
- 2016-04-01 14:31:50
- date last changed
- 2024-01-10 05:00:21
@article{116d9549-e76e-4c01-9e1d-3f4f2f9659c6,
abstract = {{Background/Aims: In response to experimental stroke, a characteristic functional and expressional upregulation of contractile G-protein-coupled receptors has been uncovered in the affected cerebral vasculature; however, the mechanism initiating this phenomenon remains unknown. Methods: Using a model of permanent distal occlusion of rat middle cerebral arteries, we investigated whether there was a regional difference in receptor-mediated contractility of segments located upstream and downstream of the occlusion site. The contractile response to endothelin, angiotensin and 5-hydroxytryptamine receptor stimulation was studied by sensitive wire myograph. Results: Only downstream segments exhibited an augmented contractile response to stimulation with each of the three ligands, with the response towards sarafotoxin 6c being especially augmented compared to sham, upstream and contralateral controls. This functional increase did not seem to relate to ischemic tissue damage, inflammatory cell infiltration or the element of reperfusion. Interestingly, immunohistochemistry did not show any difference in the level of immunoreactivity towards endothelin B (ETB) receptors between groups. Conclusion: Single artery occlusion without significant visible infarct resulted in locally increased ETB, angiotensin type 1 and 5-hydroxytryptamine 1B receptor-mediated contractile responses only in segments located downstream of the occlusion site. This suggests lack of wall stress as an initiating trigger leading to regulation of contractile response after cerebral stroke. (C) 2013 S. Karger AG, Basel}},
author = {{Rasmussen, Marianne N. P. and Hornbak, Malene and Larsen, Stine S. and Sheykhzade, Majid and Edvinsson, Lars}},
issn = {{1423-0135}},
keywords = {{5-HT1B receptor; AT(1) receptor; ETB receptor; Middle cerebral artery; Permanent occlusion; Perfusion pressure; Wall tension}},
language = {{eng}},
number = {{5}},
pages = {{396--409}},
publisher = {{Karger}},
series = {{Journal of Vascular Research}},
title = {{Permanent Distal Occlusion of Middle Cerebral Artery in Rat Causes Local Increased ETB, 5-HT1B and AT(1) Receptor-Mediated Contractility Downstream of Occlusion}},
url = {{http://dx.doi.org/10.1159/000354242}},
doi = {{10.1159/000354242}},
volume = {{50}},
year = {{2013}},
}