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Binding of extracellular matrix molecules by probiotic bacteria.

Styriak, I; Nemcová, R; Chang, Y-H and Ljungh, Åsa LU (2003) In Letters in Applied Microbiology 37(4). p.329-333
Abstract
Aims: The aim of this study was to investigate extracellular matrix (ECM) and mucin binding of selected bacterial isolates with probiotic features in comparison with commercially used probiotic bacteria.



Methods and Results: ECM molecules were immobilized in microtitre plates ( mucin and fetuin) or on the surface of latex beads. Porcine mucin was bound by all 13 probiotic strains tested with important inter-strain differences; however, fetuin binding was similar ( weak) for all 14 strains tested. Strongly positive ( three) binding of bovine fibrinogen was expressed by strains from fermented food ( Lactobacillus rhamnosus GG, L. casei Shirota and L. johnsonii La1) as well as by L. casei L. c., Lactobacillus sp. 2I3 and by... (More)
Aims: The aim of this study was to investigate extracellular matrix (ECM) and mucin binding of selected bacterial isolates with probiotic features in comparison with commercially used probiotic bacteria.



Methods and Results: ECM molecules were immobilized in microtitre plates ( mucin and fetuin) or on the surface of latex beads. Porcine mucin was bound by all 13 probiotic strains tested with important inter-strain differences; however, fetuin binding was similar ( weak) for all 14 strains tested. Strongly positive ( three) binding of bovine fibrinogen was expressed by strains from fermented food ( Lactobacillus rhamnosus GG, L. casei Shirota and L. johnsonii La1) as well as by L. casei L. c., Lactobacillus sp. 2I3 and by L. plantarum LP. The other strains expressed moderate ( 2) or weakly positive ( 1) binding of bovine fibrinogen. Strongly positive ( 3) binding of porcine fibronectin was observed only with two strains; however, all other strains also bound this molecule. Bovine lactoferrin was bound to a higher extent than transferrins.



Significance and Impact of the Study: Some animal strains ( at least L. casei L. c. and Lactobacillus sp. 2I3) are comparable with the commercially used strains with respect to their ECM binding ability. As this feature is important for probiotic bacteria to be able to colonize intestine, these strains should be considered for their wider use in fermented feed ( or probiotic preparations) for animals. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Letters in Applied Microbiology
volume
37
issue
4
pages
329 - 333
publisher
Wiley-Blackwell
external identifiers
  • wos:000185225600011
  • pmid:12969498
  • scopus:0141539313
ISSN
0266-8254
DOI
10.1046/j.1472-765X.2003.01402.x
language
English
LU publication?
yes
id
03953d51-7da2-48b6-92b1-a577424bdfcd (old id 117823)
date added to LUP
2007-07-10 14:23:34
date last changed
2017-06-11 04:36:52
@article{03953d51-7da2-48b6-92b1-a577424bdfcd,
  abstract     = {Aims: The aim of this study was to investigate extracellular matrix (ECM) and mucin binding of selected bacterial isolates with probiotic features in comparison with commercially used probiotic bacteria.<br/><br>
<br/><br>
Methods and Results: ECM molecules were immobilized in microtitre plates ( mucin and fetuin) or on the surface of latex beads. Porcine mucin was bound by all 13 probiotic strains tested with important inter-strain differences; however, fetuin binding was similar ( weak) for all 14 strains tested. Strongly positive ( three) binding of bovine fibrinogen was expressed by strains from fermented food ( Lactobacillus rhamnosus GG, L. casei Shirota and L. johnsonii La1) as well as by L. casei L. c., Lactobacillus sp. 2I3 and by L. plantarum LP. The other strains expressed moderate ( 2) or weakly positive ( 1) binding of bovine fibrinogen. Strongly positive ( 3) binding of porcine fibronectin was observed only with two strains; however, all other strains also bound this molecule. Bovine lactoferrin was bound to a higher extent than transferrins.<br/><br>
<br/><br>
Significance and Impact of the Study: Some animal strains ( at least L. casei L. c. and Lactobacillus sp. 2I3) are comparable with the commercially used strains with respect to their ECM binding ability. As this feature is important for probiotic bacteria to be able to colonize intestine, these strains should be considered for their wider use in fermented feed ( or probiotic preparations) for animals.},
  author       = {Styriak, I and Nemcová, R and Chang, Y-H and Ljungh, Åsa},
  issn         = {0266-8254},
  language     = {eng},
  number       = {4},
  pages        = {329--333},
  publisher    = {Wiley-Blackwell},
  series       = {Letters in Applied Microbiology},
  title        = {Binding of extracellular matrix molecules by probiotic bacteria.},
  url          = {http://dx.doi.org/10.1046/j.1472-765X.2003.01402.x},
  volume       = {37},
  year         = {2003},
}