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Progenitor cell-derived factors enhance photoreceptor survival in rat retinal explants.

Liljekvist Soltic, Ingela LU ; Olofsson, Jenny and Johansson, Kjell LU (2008) In Brain Research 1227. p.226-233
Abstract
Explantation of postnatal rat retinas is associated with degenerative events that show morphological similarities to human retinal degenerative disorders. The most evident morphological features are photoreceptor apoptosis involving caspase-3 and Müller cell activation. The purpose of the present study was to determine the content of protective factors in rat retinal progenitor cells and analyze the influence of the identified factors on the survival of photoreceptor cells and retinal gliosis. Tissue inhibitors of matrix metalloproteinase-1 (TIMP-1) and vascular endothelial growth factor (VEGF) were identified as putative beneficial factors, and their combined effect was examined in rat retinal explant cultures. Photoreceptor apoptosis was... (More)
Explantation of postnatal rat retinas is associated with degenerative events that show morphological similarities to human retinal degenerative disorders. The most evident morphological features are photoreceptor apoptosis involving caspase-3 and Müller cell activation. The purpose of the present study was to determine the content of protective factors in rat retinal progenitor cells and analyze the influence of the identified factors on the survival of photoreceptor cells and retinal gliosis. Tissue inhibitors of matrix metalloproteinase-1 (TIMP-1) and vascular endothelial growth factor (VEGF) were identified as putative beneficial factors, and their combined effect was examined in rat retinal explant cultures. Photoreceptor apoptosis was estimated by cell counts of cleaved caspase-3 and caspase-12 immunolabeled as well as TUNEL labeled cells. TIMP-1 and VEGF in combination significantly suppressed photoreceptor apoptosis involving caspase-3 activation. Cell counts of caspase-12 and TUNEL labeled photoreceptors showed no significant difference between the experiment and control retinas. TIMP-1 and VEGF appeared to have no effect on Müller cell activation as measured by GFAP and Ki-67 immunohistochemistry. Our data suggest that TIMP-1 and VEGF in combination promote the survival of photoreceptor cells in rat retinal explants, possibly by affecting a caspase-3 signaling pathway. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Brain Research
volume
1227
pages
226 - 233
publisher
Elsevier
external identifiers
  • wos:000259510700024
  • pmid:18621034
  • scopus:48949092914
ISSN
1872-6240
DOI
10.1016/j.brainres.2008.06.077
language
English
LU publication?
yes
id
62853158-0e5a-4f47-8747-d3290a29a71f (old id 1181266)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/18621034?dopt=Abstract
date added to LUP
2008-08-07 13:27:15
date last changed
2017-01-01 07:32:52
@article{62853158-0e5a-4f47-8747-d3290a29a71f,
  abstract     = {Explantation of postnatal rat retinas is associated with degenerative events that show morphological similarities to human retinal degenerative disorders. The most evident morphological features are photoreceptor apoptosis involving caspase-3 and Müller cell activation. The purpose of the present study was to determine the content of protective factors in rat retinal progenitor cells and analyze the influence of the identified factors on the survival of photoreceptor cells and retinal gliosis. Tissue inhibitors of matrix metalloproteinase-1 (TIMP-1) and vascular endothelial growth factor (VEGF) were identified as putative beneficial factors, and their combined effect was examined in rat retinal explant cultures. Photoreceptor apoptosis was estimated by cell counts of cleaved caspase-3 and caspase-12 immunolabeled as well as TUNEL labeled cells. TIMP-1 and VEGF in combination significantly suppressed photoreceptor apoptosis involving caspase-3 activation. Cell counts of caspase-12 and TUNEL labeled photoreceptors showed no significant difference between the experiment and control retinas. TIMP-1 and VEGF appeared to have no effect on Müller cell activation as measured by GFAP and Ki-67 immunohistochemistry. Our data suggest that TIMP-1 and VEGF in combination promote the survival of photoreceptor cells in rat retinal explants, possibly by affecting a caspase-3 signaling pathway.},
  author       = {Liljekvist Soltic, Ingela and Olofsson, Jenny and Johansson, Kjell},
  issn         = {1872-6240},
  language     = {eng},
  pages        = {226--233},
  publisher    = {Elsevier},
  series       = {Brain Research},
  title        = {Progenitor cell-derived factors enhance photoreceptor survival in rat retinal explants.},
  url          = {http://dx.doi.org/10.1016/j.brainres.2008.06.077},
  volume       = {1227},
  year         = {2008},
}