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Different elimination patterns of beta-trace protein, beta(2)-microglobulin and cystatin C in haemodialysis, haemodiafiltration and haemofiltration.

Lindström, Veronica LU ; Grubb, Anders LU ; Alquist Hegbrant, M and Christensson, Anders LU (2008) In Scandinavian Journal of Clinical & Laboratory Investigation 68. p.685-691
Abstract
Objective. Low molecular mass proteins (LMMP) are putative uraemic toxins, but their elimination is negligible in standard haemodialysis (HD). In this study, we used beta(2)-microglobulin, cystatin C and beta-trace protein, which differ in molecular mass and charge, to characterize the elimination patterns of three different dialysis modalities. Material and methods. Plasma samples were obtained at the start, 30 min after the start, at the end of the dialysis treatment and 30 min after termination of the dialysis session. Seventeen patients were treated with low-flux HD, 13 with post-dilution haemodiafiltration (HDF) and 8 with pre-dilution haemofiltration (HF). The changes in concentrations of the three LMMPs were monitored and expressed... (More)
Objective. Low molecular mass proteins (LMMP) are putative uraemic toxins, but their elimination is negligible in standard haemodialysis (HD). In this study, we used beta(2)-microglobulin, cystatin C and beta-trace protein, which differ in molecular mass and charge, to characterize the elimination patterns of three different dialysis modalities. Material and methods. Plasma samples were obtained at the start, 30 min after the start, at the end of the dialysis treatment and 30 min after termination of the dialysis session. Seventeen patients were treated with low-flux HD, 13 with post-dilution haemodiafiltration (HDF) and 8 with pre-dilution haemofiltration (HF). The changes in concentrations of the three LMMPs were monitored and expressed as percentages of the concentrations at the start of treatments. Results. Conventional HD with low-flux membranes showed a high elimination of small molecules (urea and creatinine), but did not reduce the levels of the three LMMPs studied. During HDF and HF, there was a significant decrease in the plasma levels of cystatin C (to 28 % and 44 %, respectively) (p<0.001) and of beta(2)-microglobulin (to 23 % and 33 %, respectively) (p<0.001). However, the level of beta-trace protein was significantly reduced (to 65 %) only after HDF. Conclusions . The three dialysis modalities showed significantly different elimination patterns for the LMMPs studied. Elimination of beta-trace protein was lower than those of cystatin C and beta(2)-microglobulin both in HDF and HF. beta-trace protein was only moderately eliminated by HDF and not at all by HF, and may be a useful marker in the evaluation of different convective therapies. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Scandinavian Journal of Clinical & Laboratory Investigation
volume
68
pages
685 - 691
publisher
Informa Healthcare
external identifiers
  • wos:000263221000004
  • pmid:18609120
  • scopus:57349198062
ISSN
1502-7686
DOI
10.1080/00365510802047693
language
English
LU publication?
yes
id
1f1d4a30-a85a-410d-b20e-3a2eda48cfcf (old id 1181390)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/18609120?dopt=Abstract
date added to LUP
2008-08-06 16:08:31
date last changed
2017-03-05 04:22:25
@article{1f1d4a30-a85a-410d-b20e-3a2eda48cfcf,
  abstract     = {Objective. Low molecular mass proteins (LMMP) are putative uraemic toxins, but their elimination is negligible in standard haemodialysis (HD). In this study, we used beta(2)-microglobulin, cystatin C and beta-trace protein, which differ in molecular mass and charge, to characterize the elimination patterns of three different dialysis modalities. Material and methods. Plasma samples were obtained at the start, 30 min after the start, at the end of the dialysis treatment and 30 min after termination of the dialysis session. Seventeen patients were treated with low-flux HD, 13 with post-dilution haemodiafiltration (HDF) and 8 with pre-dilution haemofiltration (HF). The changes in concentrations of the three LMMPs were monitored and expressed as percentages of the concentrations at the start of treatments. Results. Conventional HD with low-flux membranes showed a high elimination of small molecules (urea and creatinine), but did not reduce the levels of the three LMMPs studied. During HDF and HF, there was a significant decrease in the plasma levels of cystatin C (to 28 % and 44 %, respectively) (p&lt;0.001) and of beta(2)-microglobulin (to 23 % and 33 %, respectively) (p&lt;0.001). However, the level of beta-trace protein was significantly reduced (to 65 %) only after HDF. Conclusions . The three dialysis modalities showed significantly different elimination patterns for the LMMPs studied. Elimination of beta-trace protein was lower than those of cystatin C and beta(2)-microglobulin both in HDF and HF. beta-trace protein was only moderately eliminated by HDF and not at all by HF, and may be a useful marker in the evaluation of different convective therapies.},
  author       = {Lindström, Veronica and Grubb, Anders and Alquist Hegbrant, M and Christensson, Anders},
  issn         = {1502-7686},
  language     = {eng},
  pages        = {685--691},
  publisher    = {Informa Healthcare},
  series       = {Scandinavian Journal of Clinical & Laboratory Investigation},
  title        = {Different elimination patterns of beta-trace protein, beta(2)-microglobulin and cystatin C in haemodialysis, haemodiafiltration and haemofiltration.},
  url          = {http://dx.doi.org/10.1080/00365510802047693},
  volume       = {68},
  year         = {2008},
}