Advanced

Augmented Phl p 5-specific Th2 response after exposure of Dendritic Cells to allergen in complex with specific IgE compared to IgG1 and IgG4

Lundberg, Kristina LU ; Lindstedt, Malin LU ; Larsson, Kristina LU ; Dexlin Mellby, Linda LU ; Wingren, Christer LU ; Ohlin, Mats LU ; Greiff, Lennart LU and Borrebaeck, Carl LU (2008) In Clinical Immunology 128(3). p.358-365
Abstract
In this study we have elucidated the effects of allergen-specific antibodies on human DCs and T-cells. Monocyte-derived DCs from allergic patients were exposed to Phlp 5 alone or in complex with Phlp 5-specific human IgG1, IgG4 or IgE and further co-cultured with autologous memory CD4+ T-cells. We demonstrate that DCs treated with Phlp 5/IgE-complexes secrete higher levels of IL-1a, IL-6, VEGF and MCP-3 compared to Phlp 5 alone. Furthermore, we show that the ability of DCs to present allergen to memory CD4+ T-cells and induce a Th2 cytokine profile is significantly augmented when the uptake is mediated by specific IgE antibodies, whereas IgG1 and IgG4 have no such effect. The differences in cytokine profiles depending on the antibody... (More)
In this study we have elucidated the effects of allergen-specific antibodies on human DCs and T-cells. Monocyte-derived DCs from allergic patients were exposed to Phlp 5 alone or in complex with Phlp 5-specific human IgG1, IgG4 or IgE and further co-cultured with autologous memory CD4+ T-cells. We demonstrate that DCs treated with Phlp 5/IgE-complexes secrete higher levels of IL-1a, IL-6, VEGF and MCP-3 compared to Phlp 5 alone. Furthermore, we show that the ability of DCs to present allergen to memory CD4+ T-cells and induce a Th2 cytokine profile is significantly augmented when the uptake is mediated by specific IgE antibodies, whereas IgG1 and IgG4 have no such effect. The differences in cytokine profiles depending on the antibody subtype could partly explain the ability of allergic individuals to amplify allergen-specific immune response and could thus be involved in the etiology of allergic responses. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Th2, Antibody-mediated responses, T-cells, antibody arrays., Dendritic cells, IgE, Allergic Rhinitis, Phl p 5
in
Clinical Immunology
volume
128
issue
3
pages
358 - 365
publisher
Elsevier
external identifiers
  • wos:000258734100010
  • scopus:49149101497
ISSN
1521-6616
DOI
10.1016/j.clim.2008.04.011
language
English
LU publication?
yes
id
70a828a3-3728-4855-8a5d-810a8a821f79 (old id 1182509)
date added to LUP
2008-08-06 14:06:09
date last changed
2017-01-01 07:37:33
@article{70a828a3-3728-4855-8a5d-810a8a821f79,
  abstract     = {In this study we have elucidated the effects of allergen-specific antibodies on human DCs and T-cells. Monocyte-derived DCs from allergic patients were exposed to Phlp 5 alone or in complex with Phlp 5-specific human IgG1, IgG4 or IgE and further co-cultured with autologous memory CD4+ T-cells. We demonstrate that DCs treated with Phlp 5/IgE-complexes secrete higher levels of IL-1a, IL-6, VEGF and MCP-3 compared to Phlp 5 alone. Furthermore, we show that the ability of DCs to present allergen to memory CD4+ T-cells and induce a Th2 cytokine profile is significantly augmented when the uptake is mediated by specific IgE antibodies, whereas IgG1 and IgG4 have no such effect. The differences in cytokine profiles depending on the antibody subtype could partly explain the ability of allergic individuals to amplify allergen-specific immune response and could thus be involved in the etiology of allergic responses.},
  author       = {Lundberg, Kristina and Lindstedt, Malin and Larsson, Kristina and Dexlin Mellby, Linda and Wingren, Christer and Ohlin, Mats and Greiff, Lennart and Borrebaeck, Carl},
  issn         = {1521-6616},
  keyword      = {Th2,Antibody-mediated responses,T-cells,antibody arrays.,Dendritic cells,IgE,Allergic Rhinitis,Phl p 5},
  language     = {eng},
  number       = {3},
  pages        = {358--365},
  publisher    = {Elsevier},
  series       = {Clinical Immunology},
  title        = {Augmented Phl p 5-specific Th2 response after exposure of Dendritic Cells to allergen in complex with specific IgE compared to IgG1 and IgG4},
  url          = {http://dx.doi.org/10.1016/j.clim.2008.04.011},
  volume       = {128},
  year         = {2008},
}