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Energy transfer in the peridinin-chicrophyll protein complex reconstituted with mixed chlorophyll sites

Polivka, Tomas; Pascher, Torbjörn LU and Hiller, Roger G (2008) In Biophysical Journal 94(8). p.3198-3207
Abstract
We use femtosecond transient absorption spectroscopy to study chlorophyll (Chl)-Chl energy transfer in the peridinin-chlorophyll protein (PCP) reconstituted with mixtures of either chlorophyll b (Chlb) and Chld or Chla and bacteriochlorophyll a (BChla). Analysis of absorption and transient absorption spectra demonstrated that reconstitution with chlorophyll mixtures produces a significant fraction of PCP complexes that contains a different Chl in each domain of the PCP monomer. The data also suggest that binding affinity of Chla is less than that of the other three Chl species. By exciting the Chl species lying at higher energy, we obtained energy transfer times of 40 +/- 5 ps (Chlb-Chld) and 59 3 ps (Chla-BChla). The experimental values... (More)
We use femtosecond transient absorption spectroscopy to study chlorophyll (Chl)-Chl energy transfer in the peridinin-chlorophyll protein (PCP) reconstituted with mixtures of either chlorophyll b (Chlb) and Chld or Chla and bacteriochlorophyll a (BChla). Analysis of absorption and transient absorption spectra demonstrated that reconstitution with chlorophyll mixtures produces a significant fraction of PCP complexes that contains a different Chl in each domain of the PCP monomer. The data also suggest that binding affinity of Chla is less than that of the other three Chl species. By exciting the Chl species lying at higher energy, we obtained energy transfer times of 40 +/- 5 ps (Chlb-Chld) and 59 3 ps (Chla-BChla). The experimental values match those obtained from the Forster equation, 36 and 50 ps, respectively, showing that energy transfer proceeds via the Forster mechanism. Excitation of peridinin in the PCP complex reconstituted with Chla/BChla mixture provided time constants of 2.6 and 0.4 ps for the peridinin-Chla and peridinin-BChla energy transfer, matching those obtained from studies of PCP complexes reconstituted with single chlorophyll species. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biophysical Journal
volume
94
issue
8
pages
3198 - 3207
publisher
Cell Press
external identifiers
  • wos:000254420100028
  • scopus:43149098310
ISSN
1542-0086
DOI
10.1529/biophysj.107.123430
language
English
LU publication?
yes
id
e0bce95c-8a06-421f-a56a-82886eb16180 (old id 1183044)
date added to LUP
2008-09-02 11:08:41
date last changed
2017-01-01 04:56:00
@article{e0bce95c-8a06-421f-a56a-82886eb16180,
  abstract     = {We use femtosecond transient absorption spectroscopy to study chlorophyll (Chl)-Chl energy transfer in the peridinin-chlorophyll protein (PCP) reconstituted with mixtures of either chlorophyll b (Chlb) and Chld or Chla and bacteriochlorophyll a (BChla). Analysis of absorption and transient absorption spectra demonstrated that reconstitution with chlorophyll mixtures produces a significant fraction of PCP complexes that contains a different Chl in each domain of the PCP monomer. The data also suggest that binding affinity of Chla is less than that of the other three Chl species. By exciting the Chl species lying at higher energy, we obtained energy transfer times of 40 +/- 5 ps (Chlb-Chld) and 59 3 ps (Chla-BChla). The experimental values match those obtained from the Forster equation, 36 and 50 ps, respectively, showing that energy transfer proceeds via the Forster mechanism. Excitation of peridinin in the PCP complex reconstituted with Chla/BChla mixture provided time constants of 2.6 and 0.4 ps for the peridinin-Chla and peridinin-BChla energy transfer, matching those obtained from studies of PCP complexes reconstituted with single chlorophyll species.},
  author       = {Polivka, Tomas and Pascher, Torbjörn and Hiller, Roger G},
  issn         = {1542-0086},
  language     = {eng},
  number       = {8},
  pages        = {3198--3207},
  publisher    = {Cell Press},
  series       = {Biophysical Journal},
  title        = {Energy transfer in the peridinin-chicrophyll protein complex reconstituted with mixed chlorophyll sites},
  url          = {http://dx.doi.org/10.1529/biophysj.107.123430},
  volume       = {94},
  year         = {2008},
}