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Acute allergic responses induce a prompt luminal entry of airway tissue eosinophils.

Erjefält, Jonas LU ; Korsgren, Magnus LU ; Malm-Erjefält, Monika LU ; Conroy, Dolores M; Williams, Timothy J and Persson, Carl LU (2003) In American Journal of Respiratory Cell and Molecular Biology 29(4). p.439-448
Abstract
Traditionally, traffic and activation of eosinophils in asthmatic airways are thought to take place during the late-phase allergic reaction. The present study tests the hypothesis that when eosinophils are present in the tissue before allergen exposure, as in chronically inflamed asthmatic airways, acute anaphylactic reactions initiate an eosinophil response. Using a guinea-pig allergic model, where eosinophilia is present at baseline conditions, the traffic of resident eosinophils was examined in vivo immediately after allergen challenge. By 2 min after challenge, eosinophils had moved up to apical epithelial positions. Within 10 min, a marked migration of eosinophils into the airway lumen was demonstrated. Along with the allergen-induced... (More)
Traditionally, traffic and activation of eosinophils in asthmatic airways are thought to take place during the late-phase allergic reaction. The present study tests the hypothesis that when eosinophils are present in the tissue before allergen exposure, as in chronically inflamed asthmatic airways, acute anaphylactic reactions initiate an eosinophil response. Using a guinea-pig allergic model, where eosinophilia is present at baseline conditions, the traffic of resident eosinophils was examined in vivo immediately after allergen challenge. By 2 min after challenge, eosinophils had moved up to apical epithelial positions. Within 10 min, a marked migration of eosinophils into the airway lumen was demonstrated. Along with the allergen-induced egression of eosinophils, acute luminal entry of plasma proteins and eotaxin occurred. Eosinophil egression was effectively inhibited by the antiexudative drug formoterol, whereas the proexudative drug bradykinin could in naive animals evoke a prompt luminal entry of eosinophils. In conclusion, the present study demonstrates that acute allergic reactions initiate a prompt transepithelial migration of resident eosinophils. Our data further suggest that this response in part is initiated by the plasma exudation response, which may alter the transepithelial gradient of eosinophil chemoattractants including eotaxin. We propose that prompt eosinophil response is a significant component of the acute phase of allergic reactions when occurring in airways where these cells are already present in the mucosa. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
saline sodium citrate, PBS, phosphate-buffered saline, OVA, ovalbumin, EPO, eosinophil peroxidase, bronchoalveolar lavage, BAL, SSC, tissue nonspecific alkaline phosphatase, TNAP
in
American Journal of Respiratory Cell and Molecular Biology
volume
29
issue
4
pages
439 - 448
publisher
American Thoracic Society
external identifiers
  • wos:000185643900004
  • pmid:12663331
  • scopus:18344397638
ISSN
1535-4989
DOI
10.1165/rcmb.2003-0015OC
language
English
LU publication?
yes
id
21025be8-0a60-4e2c-82d0-52b9a70801e3 (old id 118520)
date added to LUP
2007-07-12 08:38:13
date last changed
2017-09-03 03:45:14
@article{21025be8-0a60-4e2c-82d0-52b9a70801e3,
  abstract     = {Traditionally, traffic and activation of eosinophils in asthmatic airways are thought to take place during the late-phase allergic reaction. The present study tests the hypothesis that when eosinophils are present in the tissue before allergen exposure, as in chronically inflamed asthmatic airways, acute anaphylactic reactions initiate an eosinophil response. Using a guinea-pig allergic model, where eosinophilia is present at baseline conditions, the traffic of resident eosinophils was examined in vivo immediately after allergen challenge. By 2 min after challenge, eosinophils had moved up to apical epithelial positions. Within 10 min, a marked migration of eosinophils into the airway lumen was demonstrated. Along with the allergen-induced egression of eosinophils, acute luminal entry of plasma proteins and eotaxin occurred. Eosinophil egression was effectively inhibited by the antiexudative drug formoterol, whereas the proexudative drug bradykinin could in naive animals evoke a prompt luminal entry of eosinophils. In conclusion, the present study demonstrates that acute allergic reactions initiate a prompt transepithelial migration of resident eosinophils. Our data further suggest that this response in part is initiated by the plasma exudation response, which may alter the transepithelial gradient of eosinophil chemoattractants including eotaxin. We propose that prompt eosinophil response is a significant component of the acute phase of allergic reactions when occurring in airways where these cells are already present in the mucosa.},
  author       = {Erjefält, Jonas and Korsgren, Magnus and Malm-Erjefält, Monika and Conroy, Dolores M and Williams, Timothy J and Persson, Carl},
  issn         = {1535-4989},
  keyword      = {saline sodium citrate,PBS,phosphate-buffered saline,OVA,ovalbumin,EPO,eosinophil peroxidase,bronchoalveolar lavage,BAL,SSC,tissue nonspecific alkaline phosphatase,TNAP},
  language     = {eng},
  number       = {4},
  pages        = {439--448},
  publisher    = {American Thoracic Society},
  series       = {American Journal of Respiratory Cell and Molecular Biology},
  title        = {Acute allergic responses induce a prompt luminal entry of airway tissue eosinophils.},
  url          = {http://dx.doi.org/10.1165/rcmb.2003-0015OC},
  volume       = {29},
  year         = {2003},
}