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Use of immobilised lipase from Candida antarctica in supercritical fluid extraction of borage (Borago officinalis L.) seed oil

Venskutonis, Petras Rimantas; Dauksas, Egidijus and Sivik, Björn LU (2008) In Food Technology and Biotechnology 46(2). p.157-163
Abstract
This study aims at the investigation of the possibilities to use immobilised lipase from Candida antarctica in supercritical fluid extraction (SFE) of borage (Borago officinalis L.) seed oil. The first series of experiments was performed to measure the extract yields obtained with pure CO2 and with the added entrainer (ethanol). The yield increased more than twice after increasing the extraction pressure from 15 to 25 MPa. Further increase to 35 MPa was less effective. The effect of the entrainer was not significant in most cases. Palmitic (13.1-16.1 %), oleic (13.4-23.8 %), linoleic (33.8-48.4 %) and linolenic (8.8-16.3 %) acids were dominant in all extracted oils. Further experiments involved the use of enzyme. In this case the first... (More)
This study aims at the investigation of the possibilities to use immobilised lipase from Candida antarctica in supercritical fluid extraction (SFE) of borage (Borago officinalis L.) seed oil. The first series of experiments was performed to measure the extract yields obtained with pure CO2 and with the added entrainer (ethanol). The yield increased more than twice after increasing the extraction pressure from 15 to 25 MPa. Further increase to 35 MPa was less effective. The effect of the entrainer was not significant in most cases. Palmitic (13.1-16.1 %), oleic (13.4-23.8 %), linoleic (33.8-48.4 %) and linolenic (8.8-16.3 %) acids were dominant in all extracted oils. Further experiments involved the use of enzyme. In this case the first extractor was loaded with ground borage seeds, the second one was filled with the enzyme. The total yield obtained at 15, 25 and 35 MPa was (8.8+/-0.2), (23.6+/-0.2) and (28.9+/-1.1) %, respectively. Thin layer chromatography (TLC) of fatty acid ethyl esters showed that the content of esters was higher in the extract obtained in one extractor system at 15 MPa, compared to 35 MPa. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
entrainer, supercritical CO2 extraction, borage, Candida antarctica
in
Food Technology and Biotechnology
volume
46
issue
2
pages
157 - 163
publisher
Faculty of Food Technology and Biotechnology University
external identifiers
  • wos:000257058900005
  • scopus:46349099662
ISSN
1330-9862
language
English
LU publication?
yes
id
a45d16de-25c0-44b2-a998-c3b9257f2da2 (old id 1186938)
alternative location
http://www.ftb.com.hr/46-157.pdf
date added to LUP
2008-09-04 10:14:20
date last changed
2017-02-26 03:42:16
@article{a45d16de-25c0-44b2-a998-c3b9257f2da2,
  abstract     = {This study aims at the investigation of the possibilities to use immobilised lipase from Candida antarctica in supercritical fluid extraction (SFE) of borage (Borago officinalis L.) seed oil. The first series of experiments was performed to measure the extract yields obtained with pure CO2 and with the added entrainer (ethanol). The yield increased more than twice after increasing the extraction pressure from 15 to 25 MPa. Further increase to 35 MPa was less effective. The effect of the entrainer was not significant in most cases. Palmitic (13.1-16.1 %), oleic (13.4-23.8 %), linoleic (33.8-48.4 %) and linolenic (8.8-16.3 %) acids were dominant in all extracted oils. Further experiments involved the use of enzyme. In this case the first extractor was loaded with ground borage seeds, the second one was filled with the enzyme. The total yield obtained at 15, 25 and 35 MPa was (8.8+/-0.2), (23.6+/-0.2) and (28.9+/-1.1) %, respectively. Thin layer chromatography (TLC) of fatty acid ethyl esters showed that the content of esters was higher in the extract obtained in one extractor system at 15 MPa, compared to 35 MPa.},
  author       = {Venskutonis, Petras Rimantas and Dauksas, Egidijus and Sivik, Björn},
  issn         = {1330-9862},
  keyword      = {entrainer,supercritical CO2 extraction,borage,Candida antarctica},
  language     = {eng},
  number       = {2},
  pages        = {157--163},
  publisher    = {Faculty of Food Technology and Biotechnology University},
  series       = {Food Technology and Biotechnology},
  title        = {Use of immobilised lipase from Candida antarctica in supercritical fluid extraction of borage (Borago officinalis L.) seed oil},
  volume       = {46},
  year         = {2008},
}