Protein family neighborhood analyzer-ProFaNA
(2023) In PeerJ 11.- Abstract
Background: Functionally related genes are well known to be often grouped in close vicinity in the genomes, particularly in prokaryotes. Notwithstanding the diverse evolutionary mechanisms leading to this phenomenon, it can be used to predict functions of uncharacterized genes. Methods: Here, we provide a simple but robust statistical approach that leverages the vast amounts of genomic data available today. Considering a protein domain as a functional unit, one can explore other functional units (domains) that significantly often occur within the genomic neighborhoods of the queried domain. This analysis can be performed across different taxonomic levels. Provisions can also be made to correct for the uneven sampling of the taxonomic... (More)
Background: Functionally related genes are well known to be often grouped in close vicinity in the genomes, particularly in prokaryotes. Notwithstanding the diverse evolutionary mechanisms leading to this phenomenon, it can be used to predict functions of uncharacterized genes. Methods: Here, we provide a simple but robust statistical approach that leverages the vast amounts of genomic data available today. Considering a protein domain as a functional unit, one can explore other functional units (domains) that significantly often occur within the genomic neighborhoods of the queried domain. This analysis can be performed across different taxonomic levels. Provisions can also be made to correct for the uneven sampling of the taxonomic space by genomic sequencing projects that often focus on large numbers of very closely related strains, e.g., pathogenic ones. To this end, an optional procedure for averaging occurrences within subtaxa is available. Results: Several examples show this approach can provide useful functional predictions for uncharacterized gene families, and how to combine this information with other approaches. The method is made available as a web server at http://bioinfo.sggw.edu.pl/neighborhood_analysis.
(Less)
- author
- Baranowski, Bartosz and Pawłowski, Krzysztof LU
- organization
- publishing date
- 2023
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Comparative genomics, Gene function prediction, Genomic neighborhoods, Protein domains
- in
- PeerJ
- volume
- 11
- article number
- e15715
- publisher
- PeerJ
- external identifiers
-
- pmid:37492397
- scopus:85170025741
- ISSN
- 2167-8359
- DOI
- 10.7717/peerj.15715
- language
- English
- LU publication?
- yes
- id
- 11892088-271f-4b11-871b-6639d80cf13c
- date added to LUP
- 2023-11-03 10:55:45
- date last changed
- 2024-04-19 03:31:44
@article{11892088-271f-4b11-871b-6639d80cf13c,
abstract = {{<p>Background: Functionally related genes are well known to be often grouped in close vicinity in the genomes, particularly in prokaryotes. Notwithstanding the diverse evolutionary mechanisms leading to this phenomenon, it can be used to predict functions of uncharacterized genes. Methods: Here, we provide a simple but robust statistical approach that leverages the vast amounts of genomic data available today. Considering a protein domain as a functional unit, one can explore other functional units (domains) that significantly often occur within the genomic neighborhoods of the queried domain. This analysis can be performed across different taxonomic levels. Provisions can also be made to correct for the uneven sampling of the taxonomic space by genomic sequencing projects that often focus on large numbers of very closely related strains, e.g., pathogenic ones. To this end, an optional procedure for averaging occurrences within subtaxa is available. Results: Several examples show this approach can provide useful functional predictions for uncharacterized gene families, and how to combine this information with other approaches. The method is made available as a web server at http://bioinfo.sggw.edu.pl/neighborhood_analysis.</p>}},
author = {{Baranowski, Bartosz and Pawłowski, Krzysztof}},
issn = {{2167-8359}},
keywords = {{Comparative genomics; Gene function prediction; Genomic neighborhoods; Protein domains}},
language = {{eng}},
publisher = {{PeerJ}},
series = {{PeerJ}},
title = {{Protein family neighborhood analyzer-ProFaNA}},
url = {{http://dx.doi.org/10.7717/peerj.15715}},
doi = {{10.7717/peerj.15715}},
volume = {{11}},
year = {{2023}},
}