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Characterization of QCM sensor surfaces coated with molecularly imprinted nanoparticles

Reimhult, Kristina; Yoshimatsu, Keiichi LU ; Risveden, Klas LU ; Chen, Si; Ye, Lei LU and Krozer, Anatol (2008) In Biosensors & Bioelectronics 23(12). p.1908-1914
Abstract
Molecularly imprinted polymers (MIPs) are gaining great interest as tailor-made recognition materials for the development of biomimetic sensors. Various approaches have been adopted to interface MIPs with different transducers, including the use of pre-made imprinted particles and the in situ preparation of thin polymer layers directly on transducer surfaces. In this work we functionalized quartz crystal microbalance (QCM) sensor crystals by coating the sensing surfaces with pre-made molecularly imprinted nanoparticles. The nanoparticles were immobilized on the QCM transducers by physical entrapment in a thin poly(ethylene terephthalate) (PET) layer that was spin-coated on the transducer surface. By controlling the deposition conditions,... (More)
Molecularly imprinted polymers (MIPs) are gaining great interest as tailor-made recognition materials for the development of biomimetic sensors. Various approaches have been adopted to interface MIPs with different transducers, including the use of pre-made imprinted particles and the in situ preparation of thin polymer layers directly on transducer surfaces. In this work we functionalized quartz crystal microbalance (QCM) sensor crystals by coating the sensing surfaces with pre-made molecularly imprinted nanoparticles. The nanoparticles were immobilized on the QCM transducers by physical entrapment in a thin poly(ethylene terephthalate) (PET) layer that was spin-coated on the transducer surface. By controlling the deposition conditions, it was possible to gain a high nanoparticle loading in a stable PET layer, allowing the recognition sites in nanoparticles to be easily accessed by the test analytes. In this work, different sensor surfaces were studied by micro-profilometry and atomic force microscopy and the functionality was evaluated using quartz crystal microbalance with dissipation (QCM-D). The molecular recognition capability of the sensors were also confirmed using radioligand binding analysis by testing their response to the presence of the test compounds, (R)- and (S)-propranolot in aqueous buffer. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
molecularly imprinted polymers, quartz crystal microbalance, nanoparticles, sensors
in
Biosensors & Bioelectronics
volume
23
issue
12
pages
1908 - 1914
publisher
Elsevier
external identifiers
  • wos:000256736300027
  • scopus:43449083182
ISSN
1873-4235
DOI
10.1016/j.bios.2008.02.011
language
English
LU publication?
yes
id
6cac8fcd-3c42-4ce6-ac86-4f3877e25ac5 (old id 1191188)
date added to LUP
2008-09-08 12:00:39
date last changed
2017-03-05 03:54:02
@article{6cac8fcd-3c42-4ce6-ac86-4f3877e25ac5,
  abstract     = {Molecularly imprinted polymers (MIPs) are gaining great interest as tailor-made recognition materials for the development of biomimetic sensors. Various approaches have been adopted to interface MIPs with different transducers, including the use of pre-made imprinted particles and the in situ preparation of thin polymer layers directly on transducer surfaces. In this work we functionalized quartz crystal microbalance (QCM) sensor crystals by coating the sensing surfaces with pre-made molecularly imprinted nanoparticles. The nanoparticles were immobilized on the QCM transducers by physical entrapment in a thin poly(ethylene terephthalate) (PET) layer that was spin-coated on the transducer surface. By controlling the deposition conditions, it was possible to gain a high nanoparticle loading in a stable PET layer, allowing the recognition sites in nanoparticles to be easily accessed by the test analytes. In this work, different sensor surfaces were studied by micro-profilometry and atomic force microscopy and the functionality was evaluated using quartz crystal microbalance with dissipation (QCM-D). The molecular recognition capability of the sensors were also confirmed using radioligand binding analysis by testing their response to the presence of the test compounds, (R)- and (S)-propranolot in aqueous buffer.},
  author       = {Reimhult, Kristina and Yoshimatsu, Keiichi and Risveden, Klas and Chen, Si and Ye, Lei and Krozer, Anatol},
  issn         = {1873-4235},
  keyword      = {molecularly imprinted polymers,quartz crystal microbalance,nanoparticles,sensors},
  language     = {eng},
  number       = {12},
  pages        = {1908--1914},
  publisher    = {Elsevier},
  series       = {Biosensors & Bioelectronics},
  title        = {Characterization of QCM sensor surfaces coated with molecularly imprinted nanoparticles},
  url          = {http://dx.doi.org/10.1016/j.bios.2008.02.011},
  volume       = {23},
  year         = {2008},
}