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Cationic agents for DNA compaction

Gaweda, Sylwia; Moran, M Carmen; Pais, Alberto A C C; Dias, Rita LU ; Schillén, Karin LU ; Lindman, Björn LU and Miguel, M Graca (2008) In Journal of Colloid and Interface Science 323(1). p.75-83
Abstract
Fluorescence microscopy was used to investigate the conformational changes of individual T4 DNA molecules induced by different compacting agents, namely the cationic surfactants, cetyltrimethylammonium bromide (CTAB) and chloride (CTAC), iron(III), lysozyme, and protamine sulfate. A protocol for establishing size estimates is suggested to obtain reproducible results. Observations show that in the presence of lysozyme and protamine sulfate, DNA molecules exhibit a conformational change from an elongated coil structure to compact globules, usually interpreted as a first-order transition. The maximum degree of compaction that is attained when iron(III) or CTAB (CrAC) are used as compacting agents is considerably smaller, and intermediate... (More)
Fluorescence microscopy was used to investigate the conformational changes of individual T4 DNA molecules induced by different compacting agents, namely the cationic surfactants, cetyltrimethylammonium bromide (CTAB) and chloride (CTAC), iron(III), lysozyme, and protamine sulfate. A protocol for establishing size estimates is suggested to obtain reproducible results. Observations show that in the presence of lysozyme and protamine sulfate, DNA molecules exhibit a conformational change from an elongated coil structure to compact globules, usually interpreted as a first-order transition. The maximum degree of compaction that is attained when iron(III) or CTAB (CrAC) are used as compacting agents is considerably smaller, and intermediate structures (less elongated coils) are visible even for high concentrations of these agents. Dynamic light scattering experiments were carried out, for some of the systems, to assess the reliability of size estimates from fluorescence microscopy. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
microscopy, fluorescence, intermediate structures, DNA compaction, coexistence, dynamic light scattering
in
Journal of Colloid and Interface Science
volume
323
issue
1
pages
75 - 83
publisher
Elsevier
external identifiers
  • wos:000256646600010
  • scopus:44149127434
ISSN
1095-7103
DOI
10.1016/j.jcis.2008.04.006
language
English
LU publication?
yes
id
bb0957bf-cbbc-47a5-89e2-10488e2bb579 (old id 1191367)
date added to LUP
2008-09-08 14:36:23
date last changed
2017-09-17 04:55:12
@article{bb0957bf-cbbc-47a5-89e2-10488e2bb579,
  abstract     = {Fluorescence microscopy was used to investigate the conformational changes of individual T4 DNA molecules induced by different compacting agents, namely the cationic surfactants, cetyltrimethylammonium bromide (CTAB) and chloride (CTAC), iron(III), lysozyme, and protamine sulfate. A protocol for establishing size estimates is suggested to obtain reproducible results. Observations show that in the presence of lysozyme and protamine sulfate, DNA molecules exhibit a conformational change from an elongated coil structure to compact globules, usually interpreted as a first-order transition. The maximum degree of compaction that is attained when iron(III) or CTAB (CrAC) are used as compacting agents is considerably smaller, and intermediate structures (less elongated coils) are visible even for high concentrations of these agents. Dynamic light scattering experiments were carried out, for some of the systems, to assess the reliability of size estimates from fluorescence microscopy.},
  author       = {Gaweda, Sylwia and Moran, M Carmen and Pais, Alberto A C C and Dias, Rita and Schillén, Karin and Lindman, Björn and Miguel, M Graca},
  issn         = {1095-7103},
  keyword      = {microscopy,fluorescence,intermediate structures,DNA compaction,coexistence,dynamic light scattering},
  language     = {eng},
  number       = {1},
  pages        = {75--83},
  publisher    = {Elsevier},
  series       = {Journal of Colloid and Interface Science},
  title        = {Cationic agents for DNA compaction},
  url          = {http://dx.doi.org/10.1016/j.jcis.2008.04.006},
  volume       = {323},
  year         = {2008},
}