Interactions between the leukaemia-associated ETO homologues of nuclear repressor proteins.
(2003) In European Journal of Haematology 71(6). p.439-447- Abstract
- The eight-twenty-one (ETO) homologues, represented by ETO, myeloid transforming gene-related protein 1 (MTGR1) and myeloid transforming gene chromosome 16 (MTG16), are nuclear repressor proteins. ETO is part of the fusion protein acute myeloid leukaemia (AML)1-ETO, resulting from the translocation (8;21). Similarly, MTG16 is disrupted to become part of AML1/MTG16 in t(16;21). The aberrant expression of these chimeras could affect interplay between ETO homologues and contribute to the leukaemogenic process. We investigated possible interactions between the ETO homologues. Ectopic co-expression in COS-cells resulted in heterodimerisation of the various ETO homologues suggesting that they may co-operate. Similarly, the chimeric oncoprotein... (More)
- The eight-twenty-one (ETO) homologues, represented by ETO, myeloid transforming gene-related protein 1 (MTGR1) and myeloid transforming gene chromosome 16 (MTG16), are nuclear repressor proteins. ETO is part of the fusion protein acute myeloid leukaemia (AML)1-ETO, resulting from the translocation (8;21). Similarly, MTG16 is disrupted to become part of AML1/MTG16 in t(16;21). The aberrant expression of these chimeras could affect interplay between ETO homologues and contribute to the leukaemogenic process. We investigated possible interactions between the ETO homologues. Ectopic co-expression in COS-cells resulted in heterodimerisation of the various ETO homologues suggesting that they may co-operate. Similarly, the chimeric oncoprotein AML1-ETO interacted with both MTGR1 and MTG16. However, results from cell lines endogenously expressing more than one ETO homologue did not demonstrate co-precipitation. Results from IP-Western and size determination by gel filtration of deletion mutants expressed in COS-cells, indicated an important role of the HHR domain for oligomerisation. A role was also suggested for the Nervy domain in the homologue interactions. Our results suggest that ETO homologues can interact with each other as well as with AML1-ETO, although it is unclear as to what extent these interactions occur in vivo. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/119324
- author
- Rondin Lindberg, Sofia LU ; Olsson, André LU ; Persson, Ann-Maj LU and Olsson, Inge LU
- organization
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- in
- European Journal of Haematology
- volume
- 71
- issue
- 6
- pages
- 439 - 447
- publisher
- Wiley-Blackwell
- external identifiers
-
- wos:000186434900007
- scopus:0344961717
- ISSN
- 1600-0609
- DOI
- 10.1046/j.0902-4441.2003.00166.x
- language
- English
- LU publication?
- yes
- id
- f208afd9-1a0b-4c5e-8eaf-2807cab34827 (old id 119324)
- alternative location
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=14703694&dopt=Abstract
- date added to LUP
- 2016-04-01 12:14:29
- date last changed
- 2022-01-27 00:55:09
@article{f208afd9-1a0b-4c5e-8eaf-2807cab34827, abstract = {{The eight-twenty-one (ETO) homologues, represented by ETO, myeloid transforming gene-related protein 1 (MTGR1) and myeloid transforming gene chromosome 16 (MTG16), are nuclear repressor proteins. ETO is part of the fusion protein acute myeloid leukaemia (AML)1-ETO, resulting from the translocation (8;21). Similarly, MTG16 is disrupted to become part of AML1/MTG16 in t(16;21). The aberrant expression of these chimeras could affect interplay between ETO homologues and contribute to the leukaemogenic process. We investigated possible interactions between the ETO homologues. Ectopic co-expression in COS-cells resulted in heterodimerisation of the various ETO homologues suggesting that they may co-operate. Similarly, the chimeric oncoprotein AML1-ETO interacted with both MTGR1 and MTG16. However, results from cell lines endogenously expressing more than one ETO homologue did not demonstrate co-precipitation. Results from IP-Western and size determination by gel filtration of deletion mutants expressed in COS-cells, indicated an important role of the HHR domain for oligomerisation. A role was also suggested for the Nervy domain in the homologue interactions. Our results suggest that ETO homologues can interact with each other as well as with AML1-ETO, although it is unclear as to what extent these interactions occur in vivo.}}, author = {{Rondin Lindberg, Sofia and Olsson, André and Persson, Ann-Maj and Olsson, Inge}}, issn = {{1600-0609}}, language = {{eng}}, number = {{6}}, pages = {{439--447}}, publisher = {{Wiley-Blackwell}}, series = {{European Journal of Haematology}}, title = {{Interactions between the leukaemia-associated ETO homologues of nuclear repressor proteins.}}, url = {{http://dx.doi.org/10.1046/j.0902-4441.2003.00166.x}}, doi = {{10.1046/j.0902-4441.2003.00166.x}}, volume = {{71}}, year = {{2003}}, }