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Presence of IgG-CD4 complexes in the circulation

Nezlin, Roald and Bengtsson, Anders LU (2008) In Immunological Investigations 37(2). p.153-162
Abstract
Many proteins of various origins are able to form complexes with Immunoglobulin G using reaction sites formed by residues of constant domains. Studies of IgG complexes of non-immune nature are important as biologically active proteins could escape from the circulation forming complexes with IgG as well as with other serum proteins whose concentration in serum is high. A quantitative characterization of circulating complexes in various diseases could give valuable information on the development of pathological processes. Immunoglobulins G are widely used for the treatment of a number of diseases and it is essential to understand what proteins are present in the preparations beside IgG. In the present study CD4 T-cell membrane glycoprotein... (More)
Many proteins of various origins are able to form complexes with Immunoglobulin G using reaction sites formed by residues of constant domains. Studies of IgG complexes of non-immune nature are important as biologically active proteins could escape from the circulation forming complexes with IgG as well as with other serum proteins whose concentration in serum is high. A quantitative characterization of circulating complexes in various diseases could give valuable information on the development of pathological processes. Immunoglobulins G are widely used for the treatment of a number of diseases and it is essential to understand what proteins are present in the preparations beside IgG. In the present study CD4 T-cell membrane glycoprotein was found in complexes with human IgG molecules isolated from donor sera as well as from sera of SLE patients via a sensitive quantitative dot-blot assay. According to immunoblotting experiments the CD4 part of the complexes had a molecular mass of about 50 kDa and is composed from all four extracellular domains. The CD4 content of the complexes varied among the studied human sera. There was no difference in IgG-CD4 complex concentration between the SLE patients and healthy controls. The data support the assumption that IgG molecules are able to act as scavengers and eliminate various proteins from the circulation including soluble CD4 protein. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
serum CD4-IgG complexes, systemic lupus erythematosus, dot-blot assay
in
Immunological Investigations
volume
37
issue
2
pages
153 - 162
publisher
Marcel Dekker
external identifiers
  • wos:000253585700005
  • scopus:40049084165
ISSN
0882-0139
DOI
10.1080/08820130701854339
language
English
LU publication?
yes
id
f0345b5c-c672-48c3-8ef3-e191e97c1e23 (old id 1193788)
date added to LUP
2008-09-09 18:11:33
date last changed
2017-01-01 05:34:11
@article{f0345b5c-c672-48c3-8ef3-e191e97c1e23,
  abstract     = {Many proteins of various origins are able to form complexes with Immunoglobulin G using reaction sites formed by residues of constant domains. Studies of IgG complexes of non-immune nature are important as biologically active proteins could escape from the circulation forming complexes with IgG as well as with other serum proteins whose concentration in serum is high. A quantitative characterization of circulating complexes in various diseases could give valuable information on the development of pathological processes. Immunoglobulins G are widely used for the treatment of a number of diseases and it is essential to understand what proteins are present in the preparations beside IgG. In the present study CD4 T-cell membrane glycoprotein was found in complexes with human IgG molecules isolated from donor sera as well as from sera of SLE patients via a sensitive quantitative dot-blot assay. According to immunoblotting experiments the CD4 part of the complexes had a molecular mass of about 50 kDa and is composed from all four extracellular domains. The CD4 content of the complexes varied among the studied human sera. There was no difference in IgG-CD4 complex concentration between the SLE patients and healthy controls. The data support the assumption that IgG molecules are able to act as scavengers and eliminate various proteins from the circulation including soluble CD4 protein.},
  author       = {Nezlin, Roald and Bengtsson, Anders},
  issn         = {0882-0139},
  keyword      = {serum CD4-IgG complexes,systemic lupus erythematosus,dot-blot assay},
  language     = {eng},
  number       = {2},
  pages        = {153--162},
  publisher    = {Marcel Dekker},
  series       = {Immunological Investigations},
  title        = {Presence of IgG-CD4 complexes in the circulation},
  url          = {http://dx.doi.org/10.1080/08820130701854339},
  volume       = {37},
  year         = {2008},
}