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Dairy product intake and risk of type 2 diabetes in EPIC-interact : A mendelian randomization study

Vissers, Linda E.T.; Sluijs, Ivonne; van der Schouw, Yvonne T.; Forouhi, Nita G.; Imamura, Fumiaki; Burgess, Stephen; Barricarte, Aurelio; Boeing, Heiner; Bonet, Catalina and Chirlaque, Maria Dolores, et al. (2019) In Diabetes Care 42(4). p.568-575
Abstract


OBJECTIVE To estimate the causal association between intake of dairy products and incident type 2 diabetes. RESEARCH DESIGN AND METHODS The analysis included 21,820 European individuals (9,686 diabetes cases) of the European Prospective Investigation into Cancer and Nutrition (EPIC)-InterAct case-cohort study. Participants were genotyped, and rs4988235 (LCT-12910C>T), a single nucleotide polymorphism (SNP) for lactase persistence (LP) that enables digestion of dairy sugar, i.e., lactose, was imputed. Baseline dietary intakes were assessed with diet questionnaires. We investigated the associations between imputed SNP dosage for rs4988235 and intake of dairy products and... (More)


OBJECTIVE To estimate the causal association between intake of dairy products and incident type 2 diabetes. RESEARCH DESIGN AND METHODS The analysis included 21,820 European individuals (9,686 diabetes cases) of the European Prospective Investigation into Cancer and Nutrition (EPIC)-InterAct case-cohort study. Participants were genotyped, and rs4988235 (LCT-12910C>T), a single nucleotide polymorphism (SNP) for lactase persistence (LP) that enables digestion of dairy sugar, i.e., lactose, was imputed. Baseline dietary intakes were assessed with diet questionnaires. We investigated the associations between imputed SNP dosage for rs4988235 and intake of dairy products and other foods through linear regression. Mendelian randomization (MR) estimates for the milk-diabetes relationship were obtained through a two-stage least squares regression. RESULTS Each additional LP allele was associated with a higher intake of milk (b 17.1 g/day, 95% CI 10.6–23.6) and milk beverages (b 2.8 g/day, 95% CI 1.0–4.5) but not with intake of other dairy products. Other dietary intakes associated with rs4988235 included fruits (b 27.0 g/day, 95% CI 212.4 to 21.7 per additional LP allele), nonalcoholic beverages (b 218.0 g/day, 95% CI 234.4 to 21.6), and wine (b 24.8 g/day, 95% CI 29.1 to 20.6). In instrumental variable analysis, LP-associated milk intake was not associated with diabetes (hazard ratio
per
15
g/day
0.99, 95% CI 0.93–1.05). CONCLUSIONS rs4988235 was associated with milk intake but not with intake of other dairy products. This MR study does not suggest that milk intake is associated with diabetes, which is consistent with previous observational and genetic associations. LP may be associated with intake of other foods as well, but owing to the modest associations, we consider it unlikely that this caused the observed null result.

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published
subject
in
Diabetes Care
volume
42
issue
4
pages
8 pages
publisher
American Diabetes Association
external identifiers
  • scopus:85063627693
ISSN
0149-5992
DOI
10.2337/dc18-2034
language
English
LU publication?
yes
id
11952cd0-1dc9-4e65-8c1e-964f2e1527f2
date added to LUP
2019-04-09 14:51:22
date last changed
2019-04-30 04:10:52
@article{11952cd0-1dc9-4e65-8c1e-964f2e1527f2,
  abstract     = {<p><br>
                                                         OBJECTIVE To estimate the causal association between intake of dairy products and incident type 2 diabetes. RESEARCH DESIGN AND METHODS The analysis included 21,820 European individuals (9,686 diabetes cases) of the European Prospective Investigation into Cancer and Nutrition (EPIC)-InterAct case-cohort study. Participants were genotyped, and rs4988235 (LCT-12910C&gt;T), a single nucleotide polymorphism (SNP) for lactase persistence (LP) that enables digestion of dairy sugar, i.e., lactose, was imputed. Baseline dietary intakes were assessed with diet questionnaires. We investigated the associations between imputed SNP dosage for rs4988235 and intake of dairy products and other foods through linear regression. Mendelian randomization (MR) estimates for the milk-diabetes relationship were obtained through a two-stage least squares regression. RESULTS Each additional LP allele was associated with a higher intake of milk (b 17.1 g/day, 95% CI 10.6–23.6) and milk beverages (b 2.8 g/day, 95% CI 1.0–4.5) but not with intake of other dairy products. Other dietary intakes associated with rs4988235 included fruits (b 27.0 g/day, 95% CI 212.4 to 21.7 per additional LP allele), nonalcoholic beverages (b 218.0 g/day, 95% CI 234.4 to 21.6), and wine (b 24.8 g/day, 95% CI 29.1 to 20.6). In instrumental variable analysis, LP-associated milk intake was not associated with diabetes (hazard ratio                             <br>
                            <sub>per</sub><br>
                                                          15                              <br>
                            <sub>g/day</sub><br>
                                                          0.99, 95% CI 0.93–1.05). CONCLUSIONS rs4988235 was associated with milk intake but not with intake of other dairy products. This MR study does not suggest that milk intake is associated with diabetes, which is consistent with previous observational and genetic associations. LP may be associated with intake of other foods as well, but owing to the modest associations, we consider it unlikely that this caused the observed null result.                         <br>
                        </p>},
  author       = {Vissers, Linda E.T. and Sluijs, Ivonne and van der Schouw, Yvonne T. and Forouhi, Nita G. and Imamura, Fumiaki and Burgess, Stephen and Barricarte, Aurelio and Boeing, Heiner and Bonet, Catalina and Chirlaque, Maria Dolores and Fagherazzi, Guy and Franks, Paul W. and Freisling, Heinz and Gunter, Marc J. and Ramón Quirós, J. and Ibsen, Daniel B. and Kaaks, Rudolf and Key, Timothy and Khaw, Kay T. and Kühn, Tilman and Mokoroa, Olatz and Nilsson, Peter M. and Overvad, Kim and Pala, Valeria and Palli, Domenico and Panico, Salvatore and Sacerdote, Carlotta and Spijkerman, Annemieke M.W. and Tjonneland, Anne and Tumino, Rosario and Rodríguez-Barranco, Miguel and Rolandsson, Olov and Riboli, Elio and Sharp, Stephen J. and Langenberg, Claudia and Wareham, Nicholas J.},
  issn         = {0149-5992},
  language     = {eng},
  number       = {4},
  pages        = {568--575},
  publisher    = {American Diabetes Association},
  series       = {Diabetes Care},
  title        = {Dairy product intake and risk of type 2 diabetes in EPIC-interact : A mendelian randomization study},
  url          = {http://dx.doi.org/10.2337/dc18-2034},
  volume       = {42},
  year         = {2019},
}