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Expression of calcium channel Ca(v)1.3 in cat spinal cord: Light and electron microscopic immunohistochemical study

Zhang, Mengliang LU ; Moller, Morten; Broman, Jonas LU ; Sukiasyan, Natalya; Wienecke, Jacob and Hultborn, Hans (2008) In Journal of Comparative Neurology 507(1). p.1109-1127
Abstract
In spinal neurons, plateau potentials serve to amplify neuronal input signals. To a large extent, the underlying persistent inward current is mediated by a subtype of the L-type calcium channel (Ca(v)1.3). In the present investigation, we have studied its distribution and cellular localization in the cat spinal cord by light and electron microscopic immunohistochemistry. The results show that Ca(v)1.3-like immunoreactivity is widely distributed in all segments of the spinal cord but that the distribution in the different laminae of the spinal gray matter varies, with the highest density of labeled neurons in lamina IX and the lowest in lamina II. The labeling intensity was highest in neuronal somata, but a certain length of the proximal... (More)
In spinal neurons, plateau potentials serve to amplify neuronal input signals. To a large extent, the underlying persistent inward current is mediated by a subtype of the L-type calcium channel (Ca(v)1.3). In the present investigation, we have studied its distribution and cellular localization in the cat spinal cord by light and electron microscopic immunohistochemistry. The results show that Ca(v)1.3-like immunoreactivity is widely distributed in all segments of the spinal cord but that the distribution in the different laminae of the spinal gray matter varies, with the highest density of labeled neurons in lamina IX and the lowest in lamina II. The labeling intensity was highest in neuronal somata, but a certain length of the proximal dendrite was also labeled. Some neuronal groups exhibited a particularly dense labeling; these include the lateral motoneuronal group in the cervical and the lumbar enlargements and the phrenic nucleus in cervical, Clarke's nucleus in lower thoracic and upper lumbar, and Onuf's nucleus in upper sacral segments. At the ultrastructural level, Ca(v)1.3-immunoreactive products were found in neuronal somata and dendrites of different sizes. In the soma, they were predominantly associated with the rough endoplasmic reticulum but some also with the plasma membrane. In dendrites, they were associated with both intracellular organelles, including microtubules and microchondria, and the plasma membrane. These results indicate that significant proportions of the neurons in cat spinal cord, including projection neurons, interneurons, and motoneurons, are endowed with ion channels that subserve persistent inward currents and act to amplify synaptic input signals. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
ultrastructure, immunohistochemistry, plateau potential, persistent inward current, L-type calcium channel, alpha ID subunit
in
Journal of Comparative Neurology
volume
507
issue
1
pages
1109 - 1127
publisher
John Wiley & Sons
external identifiers
  • wos:000252523000008
  • scopus:38949124414
ISSN
1096-9861
DOI
10.1002/cne.21595
language
English
LU publication?
yes
id
01278819-affd-49bf-ad46-58094bfc568e (old id 1199848)
date added to LUP
2008-09-11 11:35:51
date last changed
2017-07-21 14:10:38
@article{01278819-affd-49bf-ad46-58094bfc568e,
  abstract     = {In spinal neurons, plateau potentials serve to amplify neuronal input signals. To a large extent, the underlying persistent inward current is mediated by a subtype of the L-type calcium channel (Ca(v)1.3). In the present investigation, we have studied its distribution and cellular localization in the cat spinal cord by light and electron microscopic immunohistochemistry. The results show that Ca(v)1.3-like immunoreactivity is widely distributed in all segments of the spinal cord but that the distribution in the different laminae of the spinal gray matter varies, with the highest density of labeled neurons in lamina IX and the lowest in lamina II. The labeling intensity was highest in neuronal somata, but a certain length of the proximal dendrite was also labeled. Some neuronal groups exhibited a particularly dense labeling; these include the lateral motoneuronal group in the cervical and the lumbar enlargements and the phrenic nucleus in cervical, Clarke's nucleus in lower thoracic and upper lumbar, and Onuf's nucleus in upper sacral segments. At the ultrastructural level, Ca(v)1.3-immunoreactive products were found in neuronal somata and dendrites of different sizes. In the soma, they were predominantly associated with the rough endoplasmic reticulum but some also with the plasma membrane. In dendrites, they were associated with both intracellular organelles, including microtubules and microchondria, and the plasma membrane. These results indicate that significant proportions of the neurons in cat spinal cord, including projection neurons, interneurons, and motoneurons, are endowed with ion channels that subserve persistent inward currents and act to amplify synaptic input signals.},
  author       = {Zhang, Mengliang and Moller, Morten and Broman, Jonas and Sukiasyan, Natalya and Wienecke, Jacob and Hultborn, Hans},
  issn         = {1096-9861},
  keyword      = {ultrastructure,immunohistochemistry,plateau potential,persistent inward current,L-type calcium channel,alpha ID subunit},
  language     = {eng},
  number       = {1},
  pages        = {1109--1127},
  publisher    = {John Wiley & Sons},
  series       = {Journal of Comparative Neurology},
  title        = {Expression of calcium channel Ca(v)1.3 in cat spinal cord: Light and electron microscopic immunohistochemical study},
  url          = {http://dx.doi.org/10.1002/cne.21595},
  volume       = {507},
  year         = {2008},
}