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Single hollow fiber SLM extraction of polyamines followed by tosyl chloride derivatization and HPLC determination

Dziarkowska, Katarzyna; Jönsson, Jan Åke LU and Wieczorek, Piotr P (2008) In Analytica Chimica Acta 606(2). p.184-193
Abstract
Determination of polyamines in biological fluids possesses medical diagnostic relevance. Despite the vast panel of analytical methods developed for polyamines they are not applied in routine clinical usage, mainly due to the time and labor consuming sample preparation step and complicated derivatization. procedures. Thus, new simpler methods are needed. This paper describes a single hollow fiber SLM extraction method of polyarnines followed by simple pre-column derivatization with tosyl chloride and HPLC-UV analysis. The influence of different parameters such as the extraction time, organic phase composition, acceptor pH, donor pH, acceptor volume, donor volume and stirring speed on the transport parameters and enrichment was studied and... (More)
Determination of polyamines in biological fluids possesses medical diagnostic relevance. Despite the vast panel of analytical methods developed for polyamines they are not applied in routine clinical usage, mainly due to the time and labor consuming sample preparation step and complicated derivatization. procedures. Thus, new simpler methods are needed. This paper describes a single hollow fiber SLM extraction method of polyarnines followed by simple pre-column derivatization with tosyl chloride and HPLC-UV analysis. The influence of different parameters such as the extraction time, organic phase composition, acceptor pH, donor pH, acceptor volume, donor volume and stirring speed on the transport parameters and enrichment was studied and discussed. The optimized method was applied to real matrices such as urine and plasma. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
polyamines, extraction technique, hollow fiber membrane, supported liquid membrane
in
Analytica Chimica Acta
volume
606
issue
2
pages
184 - 193
publisher
Elsevier
external identifiers
  • wos:000252512300006
  • scopus:37049023407
ISSN
1873-4324
DOI
10.1016/j.aca.2007.11.014
language
English
LU publication?
yes
id
6ce4059f-a779-4c8f-951e-b0afcf9f1a61 (old id 1199997)
date added to LUP
2008-09-11 14:28:43
date last changed
2017-08-20 04:09:19
@article{6ce4059f-a779-4c8f-951e-b0afcf9f1a61,
  abstract     = {Determination of polyamines in biological fluids possesses medical diagnostic relevance. Despite the vast panel of analytical methods developed for polyamines they are not applied in routine clinical usage, mainly due to the time and labor consuming sample preparation step and complicated derivatization. procedures. Thus, new simpler methods are needed. This paper describes a single hollow fiber SLM extraction method of polyarnines followed by simple pre-column derivatization with tosyl chloride and HPLC-UV analysis. The influence of different parameters such as the extraction time, organic phase composition, acceptor pH, donor pH, acceptor volume, donor volume and stirring speed on the transport parameters and enrichment was studied and discussed. The optimized method was applied to real matrices such as urine and plasma.},
  author       = {Dziarkowska, Katarzyna and Jönsson, Jan Åke and Wieczorek, Piotr P},
  issn         = {1873-4324},
  keyword      = {polyamines,extraction technique,hollow fiber membrane,supported liquid membrane},
  language     = {eng},
  number       = {2},
  pages        = {184--193},
  publisher    = {Elsevier},
  series       = {Analytica Chimica Acta},
  title        = {Single hollow fiber SLM extraction of polyamines followed by tosyl chloride derivatization and HPLC determination},
  url          = {http://dx.doi.org/10.1016/j.aca.2007.11.014},
  volume       = {606},
  year         = {2008},
}