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IPVA-DNA cryogel membranes: Characterization, swelling, and transport studies

Papancea, Adina; Valente, Artur J M; Patachia, Silvia; Miguel, Maria LU and Lindman, Björn LU (2008) In Langmuir 24(1). p.273-279
Abstract
Double-stranded (ds) DNA from salmon testes has been incorporated into PVA hydrogels obtained by a technique of repeated freezing and thawing. The cryogels obtained are free of potential toxic species like chemical cross-linkers, and consequently, they can be used in pharmaceutical or medical applications. These cryogels show a good mechanical resistance and a white and opaque appearance caused by a heterogeneous porous structure. Encapsulated DNA molecules can be in a compacted or an extended conformation in the PVA matrix and can be controlled by tailoring the degree of crystallinity of the PVA network; this is supported by fluorescence microscopy and UV and FTIR spectroscopic studies. The two forms of encapsulated DNA were observed for... (More)
Double-stranded (ds) DNA from salmon testes has been incorporated into PVA hydrogels obtained by a technique of repeated freezing and thawing. The cryogels obtained are free of potential toxic species like chemical cross-linkers, and consequently, they can be used in pharmaceutical or medical applications. These cryogels show a good mechanical resistance and a white and opaque appearance caused by a heterogeneous porous structure. Encapsulated DNA molecules can be in a compacted or an extended conformation in the PVA matrix and can be controlled by tailoring the degree of crystallinity of the PVA network; this is supported by fluorescence microscopy and UV and FTIR spectroscopic studies. The two forms of encapsulated DNA were observed for different types of matrixes: an extended one in a more crystalline network and a globular one in a more amorphous one. Different associations of base pairs have also been observed. PVA cryogel crystallinity could be tailored by the cryogel contact with different salt solutions. Cryogel surface (scanning electron microscopy) and bulk morphology (porosimetry), swelling, DNA retention, and delivery kinetics have also been studied. All these investigations clearly show strong interactions between PVA and DNA. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Langmuir
volume
24
issue
1
pages
273 - 279
publisher
The American Chemical Society
external identifiers
  • wos:000251916100041
  • scopus:38349164364
ISSN
0743-7463
DOI
10.1021/la702639d
language
English
LU publication?
yes
id
2c194900-fd8b-4403-ae6a-e97130bc2105 (old id 1200196)
date added to LUP
2008-09-12 11:27:23
date last changed
2017-01-01 04:42:27
@article{2c194900-fd8b-4403-ae6a-e97130bc2105,
  abstract     = {Double-stranded (ds) DNA from salmon testes has been incorporated into PVA hydrogels obtained by a technique of repeated freezing and thawing. The cryogels obtained are free of potential toxic species like chemical cross-linkers, and consequently, they can be used in pharmaceutical or medical applications. These cryogels show a good mechanical resistance and a white and opaque appearance caused by a heterogeneous porous structure. Encapsulated DNA molecules can be in a compacted or an extended conformation in the PVA matrix and can be controlled by tailoring the degree of crystallinity of the PVA network; this is supported by fluorescence microscopy and UV and FTIR spectroscopic studies. The two forms of encapsulated DNA were observed for different types of matrixes: an extended one in a more crystalline network and a globular one in a more amorphous one. Different associations of base pairs have also been observed. PVA cryogel crystallinity could be tailored by the cryogel contact with different salt solutions. Cryogel surface (scanning electron microscopy) and bulk morphology (porosimetry), swelling, DNA retention, and delivery kinetics have also been studied. All these investigations clearly show strong interactions between PVA and DNA.},
  author       = {Papancea, Adina and Valente, Artur J M and Patachia, Silvia and Miguel, Maria and Lindman, Björn},
  issn         = {0743-7463},
  language     = {eng},
  number       = {1},
  pages        = {273--279},
  publisher    = {The American Chemical Society},
  series       = {Langmuir},
  title        = {IPVA-DNA cryogel membranes: Characterization, swelling, and transport studies},
  url          = {http://dx.doi.org/10.1021/la702639d},
  volume       = {24},
  year         = {2008},
}