IPVA-DNA cryogel membranes: Characterization, swelling, and transport studies
(2008) In Langmuir 24(1). p.273-279- Abstract
- Double-stranded (ds) DNA from salmon testes has been incorporated into PVA hydrogels obtained by a technique of repeated freezing and thawing. The cryogels obtained are free of potential toxic species like chemical cross-linkers, and consequently, they can be used in pharmaceutical or medical applications. These cryogels show a good mechanical resistance and a white and opaque appearance caused by a heterogeneous porous structure. Encapsulated DNA molecules can be in a compacted or an extended conformation in the PVA matrix and can be controlled by tailoring the degree of crystallinity of the PVA network; this is supported by fluorescence microscopy and UV and FTIR spectroscopic studies. The two forms of encapsulated DNA were observed for... (More)
- Double-stranded (ds) DNA from salmon testes has been incorporated into PVA hydrogels obtained by a technique of repeated freezing and thawing. The cryogels obtained are free of potential toxic species like chemical cross-linkers, and consequently, they can be used in pharmaceutical or medical applications. These cryogels show a good mechanical resistance and a white and opaque appearance caused by a heterogeneous porous structure. Encapsulated DNA molecules can be in a compacted or an extended conformation in the PVA matrix and can be controlled by tailoring the degree of crystallinity of the PVA network; this is supported by fluorescence microscopy and UV and FTIR spectroscopic studies. The two forms of encapsulated DNA were observed for different types of matrixes: an extended one in a more crystalline network and a globular one in a more amorphous one. Different associations of base pairs have also been observed. PVA cryogel crystallinity could be tailored by the cryogel contact with different salt solutions. Cryogel surface (scanning electron microscopy) and bulk morphology (porosimetry), swelling, DNA retention, and delivery kinetics have also been studied. All these investigations clearly show strong interactions between PVA and DNA. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1200196
- author
- Papancea, Adina ; Valente, Artur J M ; Patachia, Silvia ; Miguel, Maria LU and Lindman, Björn LU
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Langmuir
- volume
- 24
- issue
- 1
- pages
- 273 - 279
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- wos:000251916100041
- scopus:38349164364
- ISSN
- 0743-7463
- DOI
- 10.1021/la702639d
- language
- English
- LU publication?
- yes
- id
- 2c194900-fd8b-4403-ae6a-e97130bc2105 (old id 1200196)
- date added to LUP
- 2016-04-01 11:57:48
- date last changed
- 2022-04-28 22:34:14
@article{2c194900-fd8b-4403-ae6a-e97130bc2105,
abstract = {{Double-stranded (ds) DNA from salmon testes has been incorporated into PVA hydrogels obtained by a technique of repeated freezing and thawing. The cryogels obtained are free of potential toxic species like chemical cross-linkers, and consequently, they can be used in pharmaceutical or medical applications. These cryogels show a good mechanical resistance and a white and opaque appearance caused by a heterogeneous porous structure. Encapsulated DNA molecules can be in a compacted or an extended conformation in the PVA matrix and can be controlled by tailoring the degree of crystallinity of the PVA network; this is supported by fluorescence microscopy and UV and FTIR spectroscopic studies. The two forms of encapsulated DNA were observed for different types of matrixes: an extended one in a more crystalline network and a globular one in a more amorphous one. Different associations of base pairs have also been observed. PVA cryogel crystallinity could be tailored by the cryogel contact with different salt solutions. Cryogel surface (scanning electron microscopy) and bulk morphology (porosimetry), swelling, DNA retention, and delivery kinetics have also been studied. All these investigations clearly show strong interactions between PVA and DNA.}},
author = {{Papancea, Adina and Valente, Artur J M and Patachia, Silvia and Miguel, Maria and Lindman, Björn}},
issn = {{0743-7463}},
language = {{eng}},
number = {{1}},
pages = {{273--279}},
publisher = {{The American Chemical Society (ACS)}},
series = {{Langmuir}},
title = {{IPVA-DNA cryogel membranes: Characterization, swelling, and transport studies}},
url = {{http://dx.doi.org/10.1021/la702639d}},
doi = {{10.1021/la702639d}},
volume = {{24}},
year = {{2008}},
}