Advanced

Granule docking and cargo release in pancreatic beta-cells

Barg, Sebastian LU ; Lindqvist, Anders LU and Obermüller, Stefanie LU (2008) In Biochemical Society Transactions 36. p.294-299
Abstract
Biphasic insulin secretion is required for proper insulin action and is observed not only in vivo, but also in isolated pancreatic islets and even single beta-cells. Late events in the granule life cycle are thought to underlie this temporal pattern. In the last few years, we have therefore combined live cell imaging and electrophysiology to study insulin secretion at the level of individual granules, as they approach the plasma membrane, undergo exocytosis and finally release their insulin cargo. In the present paper, we review evidence for two emerging concepts that affect insulin secretion at the level of individual granules: (i) the existence of specialized sites where granules dock in preparation for exocytosis; and (ii)... (More)
Biphasic insulin secretion is required for proper insulin action and is observed not only in vivo, but also in isolated pancreatic islets and even single beta-cells. Late events in the granule life cycle are thought to underlie this temporal pattern. In the last few years, we have therefore combined live cell imaging and electrophysiology to study insulin secretion at the level of individual granules, as they approach the plasma membrane, undergo exocytosis and finally release their insulin cargo. In the present paper, we review evidence for two emerging concepts that affect insulin secretion at the level of individual granules: (i) the existence of specialized sites where granules dock in preparation for exocytosis; and (ii) post-exocytotic regulation of cargo release by the fusion pore. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
syntaxin, imaging, live-cell, insulin secretory granule, fusion pore, docking, exocytosis
in
Biochemical Society Transactions
volume
36
pages
294 - 299
publisher
Biochemical Society
external identifiers
  • wos:000256609000007
  • scopus:45249108706
ISSN
0300-5127
DOI
10.1042/BST0360294
language
English
LU publication?
yes
id
4844e93f-a3ae-43b0-920f-a26e08873179 (old id 1200367)
date added to LUP
2008-09-12 12:55:15
date last changed
2017-09-17 04:47:56
@article{4844e93f-a3ae-43b0-920f-a26e08873179,
  abstract     = {Biphasic insulin secretion is required for proper insulin action and is observed not only in vivo, but also in isolated pancreatic islets and even single beta-cells. Late events in the granule life cycle are thought to underlie this temporal pattern. In the last few years, we have therefore combined live cell imaging and electrophysiology to study insulin secretion at the level of individual granules, as they approach the plasma membrane, undergo exocytosis and finally release their insulin cargo. In the present paper, we review evidence for two emerging concepts that affect insulin secretion at the level of individual granules: (i) the existence of specialized sites where granules dock in preparation for exocytosis; and (ii) post-exocytotic regulation of cargo release by the fusion pore.},
  author       = {Barg, Sebastian and Lindqvist, Anders and Obermüller, Stefanie},
  issn         = {0300-5127},
  keyword      = {syntaxin,imaging,live-cell,insulin secretory granule,fusion pore,docking,exocytosis},
  language     = {eng},
  pages        = {294--299},
  publisher    = {Biochemical Society},
  series       = {Biochemical Society Transactions},
  title        = {Granule docking and cargo release in pancreatic beta-cells},
  url          = {http://dx.doi.org/10.1042/BST0360294},
  volume       = {36},
  year         = {2008},
}