Granule docking and cargo release in pancreatic beta-cells
(2008) In Biochemical Society Transactions 36. p.294-299- Abstract
- Biphasic insulin secretion is required for proper insulin action and is observed not only in vivo, but also in isolated pancreatic islets and even single beta-cells. Late events in the granule life cycle are thought to underlie this temporal pattern. In the last few years, we have therefore combined live cell imaging and electrophysiology to study insulin secretion at the level of individual granules, as they approach the plasma membrane, undergo exocytosis and finally release their insulin cargo. In the present paper, we review evidence for two emerging concepts that affect insulin secretion at the level of individual granules: (i) the existence of specialized sites where granules dock in preparation for exocytosis; and (ii)... (More)
- Biphasic insulin secretion is required for proper insulin action and is observed not only in vivo, but also in isolated pancreatic islets and even single beta-cells. Late events in the granule life cycle are thought to underlie this temporal pattern. In the last few years, we have therefore combined live cell imaging and electrophysiology to study insulin secretion at the level of individual granules, as they approach the plasma membrane, undergo exocytosis and finally release their insulin cargo. In the present paper, we review evidence for two emerging concepts that affect insulin secretion at the level of individual granules: (i) the existence of specialized sites where granules dock in preparation for exocytosis; and (ii) post-exocytotic regulation of cargo release by the fusion pore. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1200367
- author
- Barg, Sebastian LU ; Lindqvist, Anders LU and Obermüller, Stefanie LU
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- syntaxin, imaging, live-cell, insulin secretory granule, fusion pore, docking, exocytosis
- in
- Biochemical Society Transactions
- volume
- 36
- pages
- 294 - 299
- publisher
- Biochemical Society
- external identifiers
-
- wos:000256609000007
- scopus:45249108706
- pmid:18481945
- ISSN
- 0300-5127
- DOI
- 10.1042/BST0360294
- language
- English
- LU publication?
- yes
- id
- 4844e93f-a3ae-43b0-920f-a26e08873179 (old id 1200367)
- date added to LUP
- 2016-04-01 11:48:23
- date last changed
- 2022-03-20 19:17:16
@article{4844e93f-a3ae-43b0-920f-a26e08873179,
abstract = {{Biphasic insulin secretion is required for proper insulin action and is observed not only in vivo, but also in isolated pancreatic islets and even single beta-cells. Late events in the granule life cycle are thought to underlie this temporal pattern. In the last few years, we have therefore combined live cell imaging and electrophysiology to study insulin secretion at the level of individual granules, as they approach the plasma membrane, undergo exocytosis and finally release their insulin cargo. In the present paper, we review evidence for two emerging concepts that affect insulin secretion at the level of individual granules: (i) the existence of specialized sites where granules dock in preparation for exocytosis; and (ii) post-exocytotic regulation of cargo release by the fusion pore.}},
author = {{Barg, Sebastian and Lindqvist, Anders and Obermüller, Stefanie}},
issn = {{0300-5127}},
keywords = {{syntaxin; imaging; live-cell; insulin secretory granule; fusion pore; docking; exocytosis}},
language = {{eng}},
pages = {{294--299}},
publisher = {{Biochemical Society}},
series = {{Biochemical Society Transactions}},
title = {{Granule docking and cargo release in pancreatic beta-cells}},
url = {{http://dx.doi.org/10.1042/BST0360294}},
doi = {{10.1042/BST0360294}},
volume = {{36}},
year = {{2008}},
}